Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09555 and BWI76_RS09560 are separated by 151 nucleotides BWI76_RS09560 and BWI76_RS09565 are separated by 39 nucleotides BWI76_RS09565 and BWI76_RS09570 are separated by 11 nucleotides
BWI76_RS09555: BWI76_RS09555 - pyruvate oxidase, at 1,998,424 to 2,000,142
_RS09555
BWI76_RS09560: BWI76_RS09560 - DoxX family membrane protein, at 2,000,294 to 2,000,728
_RS09560
BWI76_RS09565: BWI76_RS09565 - hybrid-cluster NAD(P)-dependent oxidoreductase, at 2,000,768 to 2,001,736
_RS09565
BWI76_RS09570: BWI76_RS09570 - hydroxylamine reductase, at 2,001,748 to 2,003,400
_RS09570
Position (kb)
2000
2001
2002 Strain fitness (log2 ratio)
-1
0
1
2 at 2000.108 kb on - strand at 2000.178 kb on + strand at 2000.441 kb on - strand, within BWI76_RS09560 at 2000.602 kb on - strand, within BWI76_RS09560 at 2000.916 kb on - strand, within BWI76_RS09565 at 2000.965 kb on + strand, within BWI76_RS09565 at 2001.280 kb on + strand, within BWI76_RS09565 at 2001.288 kb on - strand, within BWI76_RS09565 at 2001.446 kb on - strand, within BWI76_RS09565 at 2001.562 kb on + strand, within BWI76_RS09565 at 2001.966 kb on + strand, within BWI76_RS09570 at 2002.022 kb on + strand, within BWI76_RS09570 at 2002.096 kb on - strand, within BWI76_RS09570 at 2002.134 kb on - strand, within BWI76_RS09570 at 2002.249 kb on - strand, within BWI76_RS09570 at 2002.592 kb on + strand, within BWI76_RS09570 at 2002.643 kb on + strand, within BWI76_RS09570 at 2002.668 kb on + strand, within BWI76_RS09570
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,000,108 - -0.5 2,000,178 + -0.7 2,000,441 - BWI76_RS09560 0.34 +0.7 2,000,602 - BWI76_RS09560 0.71 -1.7 2,000,916 - BWI76_RS09565 0.15 +0.9 2,000,965 + BWI76_RS09565 0.20 +0.5 2,001,280 + BWI76_RS09565 0.53 +1.8 2,001,288 - BWI76_RS09565 0.54 +0.6 2,001,446 - BWI76_RS09565 0.70 +0.7 2,001,562 + BWI76_RS09565 0.82 -1.1 2,001,966 + BWI76_RS09570 0.13 -1.2 2,002,022 + BWI76_RS09570 0.17 +0.1 2,002,096 - BWI76_RS09570 0.21 -1.4 2,002,134 - BWI76_RS09570 0.23 +0.0 2,002,249 - BWI76_RS09570 0.30 +0.5 2,002,592 + BWI76_RS09570 0.51 -0.9 2,002,643 + BWI76_RS09570 0.54 +0.2 2,002,668 + BWI76_RS09570 0.56 -0.0
Or see this region's nucleotide sequence