Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07715 and BWI76_RS07720 are separated by 85 nucleotides BWI76_RS07720 and BWI76_RS07725 overlap by 4 nucleotides BWI76_RS07725 and BWI76_RS07730 overlap by 4 nucleotides
BWI76_RS07715: BWI76_RS07715 - non-ribosomal peptide synthetase, at 1,633,803 to 1,637,690
_RS07715
BWI76_RS07720: BWI76_RS07720 - iron-enterobactin transporter ATP-binding protein, at 1,637,776 to 1,638,570
_RS07720
BWI76_RS07725: BWI76_RS07725 - iron-enterobactin transporter permease, at 1,638,567 to 1,639,559
_RS07725
BWI76_RS07730: BWI76_RS07730 - iron-enterobactin transporter membrane protein, at 1,639,556 to 1,640,563
_RS07730
Position (kb) 1638
1639
1640 Strain fitness (log2 ratio) -1
0
1
2 at 1637.606 kb on + strand at 1637.754 kb on + strand at 1638.416 kb on - strand, within BWI76_RS07720 at 1638.551 kb on + strand at 1638.661 kb on + strand at 1639.293 kb on + strand, within BWI76_RS07725 at 1640.043 kb on + strand, within BWI76_RS07730 at 1640.527 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,637,606 + +0.1 1,637,754 + +0.5 1,638,416 - BWI76_RS07720 0.81 +0.3 1,638,551 + +0.4 1,638,661 + +0.4 1,639,293 + BWI76_RS07725 0.73 +0.9 1,640,043 + BWI76_RS07730 0.48 +1.8 1,640,527 + +0.6
Or see this region's nucleotide sequence