Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06280

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06275 and BWI76_RS06280 overlap by 4 nucleotidesBWI76_RS06280 and BWI76_RS06285 are separated by 47 nucleotidesBWI76_RS06285 and BWI76_RS06290 are separated by 170 nucleotides BWI76_RS06275: BWI76_RS06275 - beta-carotene 15,15'-monooxygenase, at 1,319,476 to 1,322,127 _RS06275 BWI76_RS06280: BWI76_RS06280 - hypothetical protein, at 1,322,124 to 1,323,518 _RS06280 BWI76_RS06285: BWI76_RS06285 - peroxiredoxin, at 1,323,566 to 1,324,168 _RS06285 BWI76_RS06290: BWI76_RS06290 - ACP phosphodiesterase, at 1,324,339 to 1,324,920 _RS06290 Position (kb) 1322 1323 1324Strain fitness (log2 ratio) -1 0 1at 1321.305 kb on + strand, within BWI76_RS06275at 1321.305 kb on + strand, within BWI76_RS06275at 1321.532 kb on + strand, within BWI76_RS06275at 1321.540 kb on - strand, within BWI76_RS06275at 1321.611 kb on + strand, within BWI76_RS06275at 1321.813 kb on + strand, within BWI76_RS06275at 1321.893 kb on + strandat 1321.924 kb on - strandat 1321.959 kb on + strandat 1322.039 kb on + strandat 1322.152 kb on - strandat 1322.475 kb on - strand, within BWI76_RS06280at 1322.707 kb on + strand, within BWI76_RS06280at 1322.829 kb on + strand, within BWI76_RS06280at 1322.888 kb on + strand, within BWI76_RS06280at 1322.990 kb on - strand, within BWI76_RS06280at 1323.360 kb on - strand, within BWI76_RS06280at 1323.399 kb on + strandat 1323.696 kb on + strand, within BWI76_RS06285at 1323.804 kb on - strand, within BWI76_RS06285at 1323.832 kb on - strand, within BWI76_RS06285at 1323.898 kb on + strand, within BWI76_RS06285at 1323.906 kb on + strand, within BWI76_RS06285at 1324.017 kb on - strand, within BWI76_RS06285at 1324.044 kb on + strand, within BWI76_RS06285at 1324.237 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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1,321,305 + BWI76_RS06275 0.69 -0.1
1,321,305 + BWI76_RS06275 0.69 +0.1
1,321,532 + BWI76_RS06275 0.78 +0.0
1,321,540 - BWI76_RS06275 0.78 +0.3
1,321,611 + BWI76_RS06275 0.81 -0.6
1,321,813 + BWI76_RS06275 0.88 +1.3
1,321,893 + +0.6
1,321,924 - -0.8
1,321,959 + +0.0
1,322,039 + -1.1
1,322,152 - +0.2
1,322,475 - BWI76_RS06280 0.25 +0.1
1,322,707 + BWI76_RS06280 0.42 +0.9
1,322,829 + BWI76_RS06280 0.51 +0.4
1,322,888 + BWI76_RS06280 0.55 +1.5
1,322,990 - BWI76_RS06280 0.62 +0.9
1,323,360 - BWI76_RS06280 0.89 +0.5
1,323,399 + +0.3
1,323,696 + BWI76_RS06285 0.22 -0.6
1,323,804 - BWI76_RS06285 0.39 +0.7
1,323,832 - BWI76_RS06285 0.44 +0.5
1,323,898 + BWI76_RS06285 0.55 +1.1
1,323,906 + BWI76_RS06285 0.56 +0.4
1,324,017 - BWI76_RS06285 0.75 +0.5
1,324,044 + BWI76_RS06285 0.79 -1.4
1,324,237 - +0.1

Or see this region's nucleotide sequence