Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01030

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01020 and BWI76_RS01025 are separated by 3 nucleotidesBWI76_RS01025 and BWI76_RS01030 overlap by 17 nucleotidesBWI76_RS01030 and BWI76_RS01035 are separated by 18 nucleotides BWI76_RS01020: BWI76_RS01020 - DNA helicase Rep, at 207,493 to 209,511 _RS01020 BWI76_RS01025: BWI76_RS01025 - hypothetical protein, at 209,515 to 210,477 _RS01025 BWI76_RS01030: BWI76_RS01030 - cytosine/purine, uracil, thiamine, allantoin permease family protein, at 210,461 to 211,876 _RS01030 BWI76_RS01035: BWI76_RS01035 - ADP-ribosylation/crystallin J1, at 211,895 to 212,911 _RS01035 Position (kb) 210 211 212Strain fitness (log2 ratio) -1 0 1at 209.478 kb on + strandat 209.635 kb on + strand, within BWI76_RS01025at 209.705 kb on + strand, within BWI76_RS01025at 209.713 kb on - strand, within BWI76_RS01025at 209.829 kb on - strand, within BWI76_RS01025at 209.848 kb on + strand, within BWI76_RS01025at 209.865 kb on - strand, within BWI76_RS01025at 209.865 kb on - strand, within BWI76_RS01025at 209.865 kb on - strand, within BWI76_RS01025at 210.021 kb on + strand, within BWI76_RS01025at 210.031 kb on + strand, within BWI76_RS01025at 210.034 kb on - strand, within BWI76_RS01025at 210.065 kb on + strand, within BWI76_RS01025at 210.128 kb on - strand, within BWI76_RS01025at 210.128 kb on - strand, within BWI76_RS01025at 210.462 kb on - strandat 210.549 kb on + strandat 210.649 kb on + strand, within BWI76_RS01030at 210.652 kb on - strand, within BWI76_RS01030at 210.662 kb on - strand, within BWI76_RS01030at 210.677 kb on + strand, within BWI76_RS01030at 210.757 kb on + strand, within BWI76_RS01030at 211.104 kb on + strand, within BWI76_RS01030at 211.256 kb on - strand, within BWI76_RS01030at 211.441 kb on + strand, within BWI76_RS01030at 211.482 kb on - strand, within BWI76_RS01030at 211.626 kb on + strand, within BWI76_RS01030at 211.856 kb on + strandat 211.886 kb on - strandat 211.990 kb on + strandat 212.493 kb on + strand, within BWI76_RS01035at 212.568 kb on - strand, within BWI76_RS01035at 212.690 kb on + strand, within BWI76_RS01035at 212.699 kb on + strand, within BWI76_RS01035at 212.703 kb on - strand, within BWI76_RS01035at 212.707 kb on - strand, within BWI76_RS01035at 212.815 kb on + strandat 212.815 kb on + strandat 212.823 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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209,478 + -0.1
209,635 + BWI76_RS01025 0.12 +1.2
209,705 + BWI76_RS01025 0.20 -0.0
209,713 - BWI76_RS01025 0.21 -0.4
209,829 - BWI76_RS01025 0.33 +0.6
209,848 + BWI76_RS01025 0.35 -0.5
209,865 - BWI76_RS01025 0.36 +1.0
209,865 - BWI76_RS01025 0.36 +0.3
209,865 - BWI76_RS01025 0.36 -0.8
210,021 + BWI76_RS01025 0.53 +0.6
210,031 + BWI76_RS01025 0.54 +0.2
210,034 - BWI76_RS01025 0.54 -0.2
210,065 + BWI76_RS01025 0.57 -0.8
210,128 - BWI76_RS01025 0.64 +1.4
210,128 - BWI76_RS01025 0.64 +0.4
210,462 - +1.2
210,549 + -0.2
210,649 + BWI76_RS01030 0.13 +1.2
210,652 - BWI76_RS01030 0.13 -0.1
210,662 - BWI76_RS01030 0.14 +0.9
210,677 + BWI76_RS01030 0.15 +0.9
210,757 + BWI76_RS01030 0.21 +0.2
211,104 + BWI76_RS01030 0.45 -0.4
211,256 - BWI76_RS01030 0.56 +1.2
211,441 + BWI76_RS01030 0.69 +0.8
211,482 - BWI76_RS01030 0.72 +0.5
211,626 + BWI76_RS01030 0.82 +0.4
211,856 + +0.5
211,886 - +0.3
211,990 + +0.0
212,493 + BWI76_RS01035 0.59 +1.2
212,568 - BWI76_RS01035 0.66 -0.5
212,690 + BWI76_RS01035 0.78 -1.1
212,699 + BWI76_RS01035 0.79 +0.6
212,703 - BWI76_RS01035 0.79 -0.1
212,707 - BWI76_RS01035 0.80 +0.2
212,815 + +0.2
212,815 + -0.4
212,823 + +0.7

Or see this region's nucleotide sequence