Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00875

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00870 and BWI76_RS00875 are separated by 196 nucleotidesBWI76_RS00875 and BWI76_RS00880 are separated by 15 nucleotidesBWI76_RS00880 and BWI76_RS00885 are separated by 46 nucleotides BWI76_RS00870: BWI76_RS00870 - NAD(P)(+) transhydrogenase, at 179,330 to 180,730 _RS00870 BWI76_RS00875: BWI76_RS00875 - DNA-binding transcriptional regulator FabR, at 180,927 to 181,562 _RS00875 BWI76_RS00880: BWI76_RS00880 - membrane protein, at 181,578 to 181,937 _RS00880 BWI76_RS00885: BWI76_RS00885 - tRNA (uridine(54)-C5)-methyltransferase TrmA, at 181,984 to 183,084 _RS00885 Position (kb) 180 181 182Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 180.794 kb on - strandat 180.883 kb on - strandat 180.885 kb on + strandat 180.915 kb on + strandat 180.923 kb on - strandat 181.021 kb on + strand, within BWI76_RS00875at 181.021 kb on + strand, within BWI76_RS00875at 181.021 kb on + strand, within BWI76_RS00875at 181.109 kb on + strand, within BWI76_RS00875at 181.129 kb on + strand, within BWI76_RS00875at 181.294 kb on + strand, within BWI76_RS00875at 181.383 kb on + strand, within BWI76_RS00875at 181.388 kb on + strand, within BWI76_RS00875at 181.396 kb on - strand, within BWI76_RS00875at 181.444 kb on + strand, within BWI76_RS00875at 181.586 kb on - strandat 181.590 kb on + strandat 181.590 kb on + strandat 181.590 kb on + strandat 181.590 kb on + strandat 181.590 kb on + strandat 181.595 kb on + strandat 181.595 kb on + strandat 181.598 kb on - strandat 181.603 kb on - strandat 181.659 kb on + strand, within BWI76_RS00880at 181.661 kb on + strand, within BWI76_RS00880at 181.661 kb on + strand, within BWI76_RS00880at 181.886 kb on + strand, within BWI76_RS00880at 182.436 kb on + strand, within BWI76_RS00885at 182.439 kb on - strand, within BWI76_RS00885

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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180,794 - +0.1
180,883 - +0.2
180,885 + +0.4
180,915 + -0.8
180,923 - -0.5
181,021 + BWI76_RS00875 0.15 -3.7
181,021 + BWI76_RS00875 0.15 -1.0
181,021 + BWI76_RS00875 0.15 +1.2
181,109 + BWI76_RS00875 0.29 +1.2
181,129 + BWI76_RS00875 0.32 -2.5
181,294 + BWI76_RS00875 0.58 -0.8
181,383 + BWI76_RS00875 0.72 -1.6
181,388 + BWI76_RS00875 0.72 -2.6
181,396 - BWI76_RS00875 0.74 +1.2
181,444 + BWI76_RS00875 0.81 -2.8
181,586 - +0.2
181,590 + +0.6
181,590 + +1.2
181,590 + -0.8
181,590 + +0.5
181,590 + +2.7
181,595 + -0.5
181,595 + +0.6
181,598 - +2.5
181,603 - +0.4
181,659 + BWI76_RS00880 0.23 +0.8
181,661 + BWI76_RS00880 0.23 +0.3
181,661 + BWI76_RS00880 0.23 +1.2
181,886 + BWI76_RS00880 0.86 +0.6
182,436 + BWI76_RS00885 0.41 -1.2
182,439 - BWI76_RS00885 0.41 +0.5

Or see this region's nucleotide sequence