Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26025 and BWI76_RS26030 overlap by 4 nucleotides BWI76_RS26030 and BWI76_RS26035 are separated by 35 nucleotides BWI76_RS26035 and BWI76_RS26040 overlap by 8 nucleotides
BWI76_RS26025: BWI76_RS26025 - MFS transporter TsgA, at 5,377,467 to 5,378,651
_RS26025
BWI76_RS26030: BWI76_RS26030 - cytosine deaminase, at 5,378,648 to 5,379,970
_RS26030
BWI76_RS26035: BWI76_RS26035 - hypothetical protein, at 5,380,006 to 5,380,218
_RS26035
BWI76_RS26040: BWI76_RS26040 - nitrite reductase large subunit, at 5,380,211 to 5,382,754
_RS26040
Position (kb)
5378
5379
5380 Strain fitness (log2 ratio)
-1
0
1
2 at 5377.655 kb on + strand, within BWI76_RS26025 at 5377.724 kb on - strand, within BWI76_RS26025 at 5377.788 kb on - strand, within BWI76_RS26025 at 5377.790 kb on + strand, within BWI76_RS26025 at 5378.114 kb on + strand, within BWI76_RS26025 at 5378.116 kb on + strand, within BWI76_RS26025 at 5378.116 kb on + strand, within BWI76_RS26025 at 5378.124 kb on - strand, within BWI76_RS26025 at 5378.313 kb on + strand, within BWI76_RS26025 at 5378.367 kb on - strand, within BWI76_RS26025 at 5378.624 kb on + strand at 5378.661 kb on - strand at 5378.799 kb on - strand, within BWI76_RS26030 at 5378.889 kb on - strand, within BWI76_RS26030 at 5379.014 kb on - strand, within BWI76_RS26030 at 5379.024 kb on - strand at 5379.094 kb on - strand, within BWI76_RS26030 at 5379.217 kb on + strand at 5379.449 kb on - strand, within BWI76_RS26030 at 5379.526 kb on - strand, within BWI76_RS26030 at 5379.580 kb on + strand, within BWI76_RS26030 at 5379.671 kb on - strand, within BWI76_RS26030 at 5379.951 kb on - strand at 5379.971 kb on - strand at 5380.398 kb on - strand at 5380.428 kb on + strand at 5380.428 kb on + strand at 5380.488 kb on + strand, within BWI76_RS26040 at 5380.740 kb on + strand, within BWI76_RS26040 at 5380.878 kb on - strand, within BWI76_RS26040
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 5,377,655 + BWI76_RS26025 0.16 -0.7 5,377,724 - BWI76_RS26025 0.22 -0.1 5,377,788 - BWI76_RS26025 0.27 -0.1 5,377,790 + BWI76_RS26025 0.27 -0.1 5,378,114 + BWI76_RS26025 0.55 +0.4 5,378,116 + BWI76_RS26025 0.55 -0.1 5,378,116 + BWI76_RS26025 0.55 -0.8 5,378,124 - BWI76_RS26025 0.55 +0.5 5,378,313 + BWI76_RS26025 0.71 +1.8 5,378,367 - BWI76_RS26025 0.76 +0.3 5,378,624 + -1.0 5,378,661 - -0.2 5,378,799 - BWI76_RS26030 0.11 +0.7 5,378,889 - BWI76_RS26030 0.18 +0.6 5,379,014 - BWI76_RS26030 0.28 +1.2 5,379,024 - +0.6 5,379,094 - BWI76_RS26030 0.34 +2.2 5,379,217 + +1.2 5,379,449 - BWI76_RS26030 0.61 +1.2 5,379,526 - BWI76_RS26030 0.66 +0.3 5,379,580 + BWI76_RS26030 0.70 +1.0 5,379,671 - BWI76_RS26030 0.77 +0.0 5,379,951 - +0.3 5,379,971 - +0.3 5,380,398 - +0.3 5,380,428 + +0.3 5,380,428 + +1.2 5,380,488 + BWI76_RS26040 0.11 -0.4 5,380,740 + BWI76_RS26040 0.21 +0.8 5,380,878 - BWI76_RS26040 0.26 -1.0
Or see this region's nucleotide sequence