Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25290 and BWI76_RS25295 overlap by 4 nucleotides BWI76_RS25295 and BWI76_RS25300 are separated by 234 nucleotides BWI76_RS25300 and BWI76_RS25305 are separated by 142 nucleotides
BWI76_RS25290: BWI76_RS25290 - monofunctional biosynthetic peptidoglycan transglycosylase, at 5,255,367 to 5,256,092
_RS25290
BWI76_RS25295: BWI76_RS25295 - glutamine amidotransferase, at 5,256,089 to 5,256,742
_RS25295
BWI76_RS25300: BWI76_RS25300 - hybrid sensor histidine kinase/response regulator, at 5,256,977 to 5,259,316
_RS25300
BWI76_RS25305: BWI76_RS25305 - TIGR01212 family radical SAM protein, at 5,259,459 to 5,260,394
_RS25305
Position (kb)
5256
5257
5258
5259
5260 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5255.989 kb on + strand, within BWI76_RS25290 at 5255.989 kb on + strand, within BWI76_RS25290 at 5255.994 kb on + strand, within BWI76_RS25290 at 5255.997 kb on - strand, within BWI76_RS25290 at 5256.013 kb on + strand, within BWI76_RS25290 at 5256.021 kb on - strand at 5256.347 kb on + strand, within BWI76_RS25295 at 5256.468 kb on + strand, within BWI76_RS25295 at 5256.521 kb on - strand, within BWI76_RS25295 at 5256.556 kb on + strand, within BWI76_RS25295 at 5256.724 kb on - strand at 5256.729 kb on - strand at 5256.745 kb on + strand at 5256.960 kb on + strand at 5257.183 kb on - strand at 5257.207 kb on - strand at 5257.453 kb on - strand, within BWI76_RS25300 at 5257.781 kb on - strand, within BWI76_RS25300 at 5258.587 kb on + strand, within BWI76_RS25300 at 5258.761 kb on + strand, within BWI76_RS25300 at 5258.990 kb on - strand, within BWI76_RS25300 at 5259.039 kb on - strand, within BWI76_RS25300 at 5259.105 kb on - strand at 5259.170 kb on - strand at 5259.189 kb on - strand at 5259.282 kb on + strand at 5259.290 kb on - strand at 5259.290 kb on - strand at 5259.416 kb on + strand at 5259.439 kb on + strand at 5259.512 kb on - strand at 5259.568 kb on + strand, within BWI76_RS25305 at 5259.571 kb on - strand, within BWI76_RS25305 at 5259.718 kb on - strand, within BWI76_RS25305 at 5259.758 kb on + strand, within BWI76_RS25305 at 5259.766 kb on - strand, within BWI76_RS25305 at 5259.937 kb on + strand, within BWI76_RS25305 at 5259.969 kb on + strand, within BWI76_RS25305 at 5259.985 kb on + strand, within BWI76_RS25305
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 5,255,989 + BWI76_RS25290 0.86 -0.8 5,255,989 + BWI76_RS25290 0.86 +2.7 5,255,994 + BWI76_RS25290 0.86 -0.4 5,255,997 - BWI76_RS25290 0.87 +0.3 5,256,013 + BWI76_RS25290 0.89 +1.0 5,256,021 - +0.8 5,256,347 + BWI76_RS25295 0.39 -0.7 5,256,468 + BWI76_RS25295 0.58 -0.4 5,256,521 - BWI76_RS25295 0.66 -0.0 5,256,556 + BWI76_RS25295 0.71 +0.5 5,256,724 - +0.1 5,256,729 - +0.5 5,256,745 + +1.1 5,256,960 + +0.4 5,257,183 - +0.2 5,257,207 - -0.4 5,257,453 - BWI76_RS25300 0.20 -1.2 5,257,781 - BWI76_RS25300 0.34 -0.4 5,258,587 + BWI76_RS25300 0.69 -1.4 5,258,761 + BWI76_RS25300 0.76 +0.1 5,258,990 - BWI76_RS25300 0.86 -1.2 5,259,039 - BWI76_RS25300 0.88 -2.0 5,259,105 - -2.8 5,259,170 - +0.5 5,259,189 - +0.1 5,259,282 + -0.1 5,259,290 - -0.0 5,259,290 - -0.5 5,259,416 + -0.6 5,259,439 + -0.4 5,259,512 - +1.1 5,259,568 + BWI76_RS25305 0.12 -1.0 5,259,571 - BWI76_RS25305 0.12 -1.2 5,259,718 - BWI76_RS25305 0.28 +0.1 5,259,758 + BWI76_RS25305 0.32 -1.4 5,259,766 - BWI76_RS25305 0.33 -0.1 5,259,937 + BWI76_RS25305 0.51 +0.0 5,259,969 + BWI76_RS25305 0.54 +0.1 5,259,985 + BWI76_RS25305 0.56 -0.3
Or see this region's nucleotide sequence