Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24265 and BWI76_RS24270 are separated by 121 nucleotides BWI76_RS24270 and BWI76_RS24275 are separated by 142 nucleotides BWI76_RS24275 and BWI76_RS24280 are separated by 6 nucleotides BWI76_RS24280 and BWI76_RS24285 are separated by 251 nucleotides
BWI76_RS24265: BWI76_RS24265 - dienelactone hydrolase, at 5,056,261 to 5,057,148
_RS24265
BWI76_RS24270: BWI76_RS24270 - membrane protein, at 5,057,270 to 5,057,764
_RS24270
BWI76_RS24275: BWI76_RS24275 - biopolymer transport protein ExbD, at 5,057,907 to 5,058,332
_RS24275
BWI76_RS24280: BWI76_RS24280 - biopolymer transporter ExbB, at 5,058,339 to 5,059,070
_RS24280
BWI76_RS24285: BWI76_RS24285 - cystathionine beta-lyase, at 5,059,322 to 5,060,509
_RS24285
Position (kb) 5057
5058
5059 Strain fitness (log2 ratio) -2
-1
0
1 at 5056.965 kb on + strand, within BWI76_RS24265 at 5057.008 kb on - strand, within BWI76_RS24265 at 5057.306 kb on - strand at 5057.357 kb on - strand, within BWI76_RS24270 at 5057.554 kb on + strand, within BWI76_RS24270 at 5057.598 kb on + strand, within BWI76_RS24270 at 5057.690 kb on - strand, within BWI76_RS24270 at 5057.794 kb on + strand at 5057.794 kb on + strand at 5057.872 kb on + strand at 5057.879 kb on - strand at 5057.974 kb on + strand, within BWI76_RS24275 at 5057.988 kb on - strand, within BWI76_RS24275 at 5058.135 kb on - strand, within BWI76_RS24275 at 5058.382 kb on - strand at 5058.598 kb on - strand, within BWI76_RS24280 at 5058.746 kb on + strand, within BWI76_RS24280 at 5058.805 kb on - strand, within BWI76_RS24280 at 5058.990 kb on + strand, within BWI76_RS24280 at 5059.184 kb on + strand at 5059.256 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 5,056,965 + BWI76_RS24265 0.79 -0.2 5,057,008 - BWI76_RS24265 0.84 -0.5 5,057,306 - +0.6 5,057,357 - BWI76_RS24270 0.18 +0.6 5,057,554 + BWI76_RS24270 0.57 +1.0 5,057,598 + BWI76_RS24270 0.66 -2.6 5,057,690 - BWI76_RS24270 0.85 +0.3 5,057,794 + -1.3 5,057,794 + -0.6 5,057,872 + +0.1 5,057,879 - +0.6 5,057,974 + BWI76_RS24275 0.16 -0.9 5,057,988 - BWI76_RS24275 0.19 -1.5 5,058,135 - BWI76_RS24275 0.54 -1.1 5,058,382 - +0.5 5,058,598 - BWI76_RS24280 0.35 +0.2 5,058,746 + BWI76_RS24280 0.56 +1.1 5,058,805 - BWI76_RS24280 0.64 +0.3 5,058,990 + BWI76_RS24280 0.89 +0.8 5,059,184 + -0.2 5,059,256 - -1.4
Or see this region's nucleotide sequence