Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23125

Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23115 and BWI76_RS23120 are separated by 10 nucleotidesBWI76_RS23120 and BWI76_RS23125 are separated by 14 nucleotidesBWI76_RS23125 and BWI76_RS23130 are separated by 15 nucleotides BWI76_RS23115: BWI76_RS23115 - propanediol dehydratase large subunit, at 4,818,711 to 4,820,375 _RS23115 BWI76_RS23120: BWI76_RS23120 - propanediol dehydratase medium subunit, at 4,820,386 to 4,821,045 _RS23120 BWI76_RS23125: BWI76_RS23125 - propanediol dehydratase small subunit, at 4,821,060 to 4,821,581 _RS23125 BWI76_RS23130: BWI76_RS23130 - diol dehydratase reactivase subunit alpha, at 4,821,597 to 4,823,429 _RS23130 Position (kb) 4821 4822Strain fitness (log2 ratio) -2 -1 0 1at 4820.086 kb on - strand, within BWI76_RS23115at 4820.093 kb on + strand, within BWI76_RS23115at 4820.174 kb on - strand, within BWI76_RS23115at 4820.192 kb on + strand, within BWI76_RS23115at 4820.222 kb on - strandat 4820.350 kb on + strandat 4820.352 kb on + strandat 4820.458 kb on - strand, within BWI76_RS23120at 4820.851 kb on + strand, within BWI76_RS23120at 4820.920 kb on + strand, within BWI76_RS23120at 4821.096 kb on + strandat 4821.290 kb on + strand, within BWI76_RS23125at 4821.448 kb on - strand, within BWI76_RS23125at 4821.582 kb on + strandat 4821.919 kb on + strand, within BWI76_RS23130at 4821.951 kb on - strand, within BWI76_RS23130at 4822.237 kb on - strand, within BWI76_RS23130at 4822.237 kb on - strand, within BWI76_RS23130at 4822.242 kb on + strand, within BWI76_RS23130at 4822.466 kb on - strand, within BWI76_RS23130at 4822.533 kb on + strand, within BWI76_RS23130at 4822.538 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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4,820,086 - BWI76_RS23115 0.83 -1.0
4,820,093 + BWI76_RS23115 0.83 +0.3
4,820,174 - BWI76_RS23115 0.88 -0.0
4,820,192 + BWI76_RS23115 0.89 +1.1
4,820,222 - +0.6
4,820,350 + +0.3
4,820,352 + +0.4
4,820,458 - BWI76_RS23120 0.11 +0.5
4,820,851 + BWI76_RS23120 0.70 +0.4
4,820,920 + BWI76_RS23120 0.81 -0.4
4,821,096 + -1.3
4,821,290 + BWI76_RS23125 0.44 -1.1
4,821,448 - BWI76_RS23125 0.74 -1.5
4,821,582 + +0.3
4,821,919 + BWI76_RS23130 0.18 +0.2
4,821,951 - BWI76_RS23130 0.19 -0.4
4,822,237 - BWI76_RS23130 0.35 -2.6
4,822,237 - BWI76_RS23130 0.35 -0.1
4,822,242 + BWI76_RS23130 0.35 +0.2
4,822,466 - BWI76_RS23130 0.47 -0.8
4,822,533 + BWI76_RS23130 0.51 -0.0
4,822,538 + -0.5

Or see this region's nucleotide sequence