Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20370 and BWI76_RS20375 are separated by 131 nucleotides BWI76_RS20375 and BWI76_RS20380 are separated by 27 nucleotides BWI76_RS20380 and BWI76_RS20385 overlap by 1 nucleotides
BWI76_RS20370: BWI76_RS20370 - acetyl-CoA carboxylase carboxyl transferase subunit beta, at 4,264,098 to 4,265,075
_RS20370
BWI76_RS20375: BWI76_RS20375 - membrane protein, at 4,265,207 to 4,265,866
_RS20375
BWI76_RS20380: BWI76_RS20380 - tRNA pseudouridine(38-40) synthase TruA, at 4,265,894 to 4,266,706
_RS20380
BWI76_RS20385: BWI76_RS20385 - aspartate-semialdehyde dehydrogenase, at 4,266,706 to 4,267,719
_RS20385
Position (kb) 4265
4266
4267 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4265.404 kb on + strand, within BWI76_RS20375 at 4265.756 kb on - strand, within BWI76_RS20375 at 4265.878 kb on + strand at 4265.909 kb on - strand at 4266.308 kb on + strand, within BWI76_RS20380 at 4266.788 kb on + strand at 4266.793 kb on + strand at 4266.921 kb on + strand, within BWI76_RS20385 at 4266.974 kb on - strand, within BWI76_RS20385 at 4267.035 kb on - strand, within BWI76_RS20385 at 4267.590 kb on - strand, within BWI76_RS20385
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,265,404 + BWI76_RS20375 0.30 +0.4 4,265,756 - BWI76_RS20375 0.83 +1.0 4,265,878 + -1.6 4,265,909 - -0.9 4,266,308 + BWI76_RS20380 0.51 -1.0 4,266,788 + -2.9 4,266,793 + -0.7 4,266,921 + BWI76_RS20385 0.21 -2.6 4,266,974 - BWI76_RS20385 0.26 -1.9 4,267,035 - BWI76_RS20385 0.32 -0.5 4,267,590 - BWI76_RS20385 0.87 +0.4
Or see this region's nucleotide sequence