Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19770

Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19765 and BWI76_RS19770 are separated by 426 nucleotidesBWI76_RS19770 and BWI76_RS19775 are separated by 190 nucleotides BWI76_RS19765: BWI76_RS19765 - membrane protein, at 4,132,161 to 4,132,868 _RS19765 BWI76_RS19770: BWI76_RS19770 - bifunctional murein DD-endopeptidase/murein LD-carboxypeptidase, at 4,133,295 to 4,133,864 _RS19770 BWI76_RS19775: BWI76_RS19775 - phage resistance protein, at 4,134,055 to 4,135,617 _RS19775 Position (kb) 4133 4134Strain fitness (log2 ratio) -3 -2 -1 0 1at 4132.295 kb on + strand, within BWI76_RS19765at 4132.314 kb on + strand, within BWI76_RS19765at 4132.374 kb on + strand, within BWI76_RS19765at 4132.382 kb on - strand, within BWI76_RS19765at 4132.446 kb on + strand, within BWI76_RS19765at 4132.537 kb on - strand, within BWI76_RS19765at 4132.585 kb on + strand, within BWI76_RS19765at 4132.729 kb on - strand, within BWI76_RS19765at 4132.775 kb on - strand, within BWI76_RS19765at 4132.792 kb on - strand, within BWI76_RS19765at 4132.810 kb on + strandat 4132.900 kb on + strandat 4133.020 kb on - strandat 4133.023 kb on + strandat 4133.124 kb on - strandat 4133.124 kb on - strandat 4133.124 kb on - strandat 4133.281 kb on + strandat 4133.289 kb on - strandat 4133.289 kb on - strandat 4133.358 kb on + strand, within BWI76_RS19770at 4133.366 kb on - strand, within BWI76_RS19770at 4133.847 kb on + strandat 4134.051 kb on + strandat 4134.059 kb on - strandat 4134.202 kb on + strandat 4134.202 kb on + strandat 4134.202 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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4,132,295 + BWI76_RS19765 0.19 +0.3
4,132,314 + BWI76_RS19765 0.22 -1.3
4,132,374 + BWI76_RS19765 0.30 -0.2
4,132,382 - BWI76_RS19765 0.31 +0.5
4,132,446 + BWI76_RS19765 0.40 +0.3
4,132,537 - BWI76_RS19765 0.53 -0.6
4,132,585 + BWI76_RS19765 0.60 -0.4
4,132,729 - BWI76_RS19765 0.80 +0.7
4,132,775 - BWI76_RS19765 0.87 +0.1
4,132,792 - BWI76_RS19765 0.89 -0.5
4,132,810 + +0.0
4,132,900 + -0.1
4,133,020 - -0.2
4,133,023 + -0.2
4,133,124 - +0.0
4,133,124 - -0.7
4,133,124 - +0.8
4,133,281 + -1.0
4,133,289 - -0.5
4,133,289 - -0.8
4,133,358 + BWI76_RS19770 0.11 -3.6
4,133,366 - BWI76_RS19770 0.12 -0.8
4,133,847 + +0.5
4,134,051 + +1.0
4,134,059 - +0.7
4,134,202 + +1.0
4,134,202 + -0.7
4,134,202 + +0.7

Or see this region's nucleotide sequence