Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16475 and BWI76_RS16480 are separated by 79 nucleotides BWI76_RS16480 and BWI76_RS16485 are separated by 106 nucleotides BWI76_RS16485 and BWI76_RS16490 are separated by 51 nucleotides
BWI76_RS16475: BWI76_RS16475 - alkene reductase, at 3,416,096 to 3,417,193
_RS16475
BWI76_RS16480: BWI76_RS16480 - lactoylglutathione lyase, at 3,417,273 to 3,417,680
_RS16480
BWI76_RS16485: BWI76_RS16485 - ribonuclease T, at 3,417,787 to 3,418,431
_RS16485
BWI76_RS16490: BWI76_RS16490 - glutaredoxin, at 3,418,483 to 3,418,830
_RS16490
Position (kb)
3417
3418 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3416.290 kb on + strand, within BWI76_RS16475 at 3416.325 kb on + strand, within BWI76_RS16475 at 3416.325 kb on + strand, within BWI76_RS16475 at 3416.333 kb on - strand, within BWI76_RS16475 at 3416.717 kb on + strand, within BWI76_RS16475 at 3416.725 kb on - strand, within BWI76_RS16475 at 3417.036 kb on + strand, within BWI76_RS16475 at 3417.229 kb on + strand at 3417.237 kb on - strand at 3417.334 kb on + strand, within BWI76_RS16480 at 3417.476 kb on + strand, within BWI76_RS16480 at 3417.527 kb on - strand, within BWI76_RS16480 at 3417.658 kb on + strand at 3417.804 kb on + strand at 3418.251 kb on - strand, within BWI76_RS16485 at 3418.312 kb on + strand, within BWI76_RS16485 at 3418.612 kb on - strand, within BWI76_RS16490
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,416,290 + BWI76_RS16475 0.18 +0.1 3,416,325 + BWI76_RS16475 0.21 +0.7 3,416,325 + BWI76_RS16475 0.21 +0.4 3,416,333 - BWI76_RS16475 0.22 +0.1 3,416,717 + BWI76_RS16475 0.57 +3.0 3,416,725 - BWI76_RS16475 0.57 +1.0 3,417,036 + BWI76_RS16475 0.86 -1.5 3,417,229 + -0.3 3,417,237 - -0.8 3,417,334 + BWI76_RS16480 0.15 -0.6 3,417,476 + BWI76_RS16480 0.50 -1.4 3,417,527 - BWI76_RS16480 0.62 -2.2 3,417,658 + +0.1 3,417,804 + +1.0 3,418,251 - BWI76_RS16485 0.72 +1.0 3,418,312 + BWI76_RS16485 0.81 -1.7 3,418,612 - BWI76_RS16490 0.37 -0.5
Or see this region's nucleotide sequence