Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15125 and BWI76_RS15130 overlap by 4 nucleotides BWI76_RS15130 and BWI76_RS15135 are separated by 300 nucleotides BWI76_RS15135 and BWI76_RS15140 are separated by 35 nucleotides
BWI76_RS15125: BWI76_RS15125 - acetyltransferase, at 3,170,617 to 3,171,195
_RS15125
BWI76_RS15130: BWI76_RS15130 - hypothetical protein, at 3,171,192 to 3,171,638
_RS15130
BWI76_RS15135: BWI76_RS15135 - hypothetical protein, at 3,171,939 to 3,172,229
_RS15135
BWI76_RS15140: BWI76_RS15140 - CusA/CzcA family heavy metal efflux RND transporter, at 3,172,265 to 3,175,378
_RS15140
Position (kb)
3171
3172
3173 Strain fitness (log2 ratio)
-1
0
1 at 3170.991 kb on + strand, within BWI76_RS15125 at 3171.183 kb on - strand at 3171.359 kb on + strand, within BWI76_RS15130 at 3171.413 kb on - strand, within BWI76_RS15130 at 3171.745 kb on - strand at 3171.904 kb on - strand at 3172.060 kb on - strand, within BWI76_RS15135 at 3172.090 kb on - strand, within BWI76_RS15135 at 3172.159 kb on + strand, within BWI76_RS15135 at 3172.167 kb on - strand, within BWI76_RS15135 at 3172.346 kb on + strand at 3172.542 kb on - strand at 3172.695 kb on + strand, within BWI76_RS15140 at 3172.703 kb on - strand, within BWI76_RS15140 at 3172.801 kb on + strand, within BWI76_RS15140
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,170,991 + BWI76_RS15125 0.65 +0.9 3,171,183 - +0.3 3,171,359 + BWI76_RS15130 0.37 -0.1 3,171,413 - BWI76_RS15130 0.49 -0.3 3,171,745 - -0.8 3,171,904 - +0.6 3,172,060 - BWI76_RS15135 0.42 +1.6 3,172,090 - BWI76_RS15135 0.52 +0.5 3,172,159 + BWI76_RS15135 0.76 +0.2 3,172,167 - BWI76_RS15135 0.78 +0.3 3,172,346 + +0.5 3,172,542 - +0.2 3,172,695 + BWI76_RS15140 0.14 +0.2 3,172,703 - BWI76_RS15140 0.14 +0.3 3,172,801 + BWI76_RS15140 0.17 +0.4
Or see this region's nucleotide sequence