Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12485 and BWI76_RS12490 are separated by 116 nucleotides BWI76_RS12490 and BWI76_RS12495 are separated by 41 nucleotides BWI76_RS12495 and BWI76_RS12500 are separated by 101 nucleotides
BWI76_RS12485: BWI76_RS12485 - TyrR family transcriptional regulator, at 2,607,363 to 2,608,904
_RS12485
BWI76_RS12490: BWI76_RS12490 - AbrB family transcriptional regulator, at 2,609,021 to 2,610,076
_RS12490
BWI76_RS12495: BWI76_RS12495 - 2-Cys peroxiredoxin, at 2,610,118 to 2,610,627
_RS12495
BWI76_RS12500: BWI76_RS12500 - dipeptide epimerase, at 2,610,729 to 2,611,694
_RS12500
Position (kb)
2609
2610
2611 Strain fitness (log2 ratio)
-1
0
1 at 2608.100 kb on - strand, within BWI76_RS12485 at 2608.657 kb on + strand, within BWI76_RS12485 at 2608.657 kb on + strand, within BWI76_RS12485 at 2608.820 kb on + strand at 2608.855 kb on + strand at 2608.863 kb on - strand at 2608.878 kb on - strand at 2609.419 kb on + strand, within BWI76_RS12490 at 2609.567 kb on - strand, within BWI76_RS12490 at 2609.661 kb on + strand, within BWI76_RS12490 at 2609.745 kb on - strand, within BWI76_RS12490 at 2609.988 kb on - strand at 2610.154 kb on + strand at 2610.527 kb on + strand, within BWI76_RS12495 at 2611.041 kb on - strand, within BWI76_RS12500 at 2611.055 kb on + strand, within BWI76_RS12500
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,608,100 - BWI76_RS12485 0.48 -1.4 2,608,657 + BWI76_RS12485 0.84 +0.5 2,608,657 + BWI76_RS12485 0.84 -1.4 2,608,820 + -0.2 2,608,855 + +0.4 2,608,863 - +0.8 2,608,878 - +1.3 2,609,419 + BWI76_RS12490 0.38 +0.0 2,609,567 - BWI76_RS12490 0.52 +0.6 2,609,661 + BWI76_RS12490 0.61 +0.9 2,609,745 - BWI76_RS12490 0.69 +0.7 2,609,988 - +0.5 2,610,154 + +0.0 2,610,527 + BWI76_RS12495 0.80 -0.9 2,611,041 - BWI76_RS12500 0.32 +0.3 2,611,055 + BWI76_RS12500 0.34 -1.4
Or see this region's nucleotide sequence