Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12040 and BWI76_RS12045 overlap by 4 nucleotides BWI76_RS12045 and BWI76_RS12050 overlap by 8 nucleotides BWI76_RS12050 and BWI76_RS12055 are separated by 34 nucleotides
BWI76_RS12040: BWI76_RS12040 - hypothetical protein, at 2,531,666 to 2,531,974
_RS12040
BWI76_RS12045: BWI76_RS12045 - hypothetical protein, at 2,531,971 to 2,532,690
_RS12045
BWI76_RS12050: BWI76_RS12050 - hypothetical protein, at 2,532,683 to 2,533,036
_RS12050
BWI76_RS12055: BWI76_RS12055 - DNA single-strand annealing protein RecT-like protein, at 2,533,071 to 2,534,180
_RS12055
Position (kb)
2532
2533
2534 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2531.708 kb on + strand, within BWI76_RS12040 at 2531.983 kb on + strand at 2532.114 kb on - strand, within BWI76_RS12045 at 2532.233 kb on + strand, within BWI76_RS12045 at 2532.627 kb on + strand at 2532.627 kb on + strand at 2532.642 kb on + strand at 2532.645 kb on - strand at 2532.869 kb on + strand, within BWI76_RS12050 at 2532.877 kb on - strand, within BWI76_RS12050 at 2532.937 kb on + strand, within BWI76_RS12050 at 2532.956 kb on - strand at 2532.989 kb on - strand, within BWI76_RS12050 at 2533.106 kb on - strand at 2533.276 kb on + strand, within BWI76_RS12055 at 2533.531 kb on - strand, within BWI76_RS12055 at 2533.662 kb on - strand, within BWI76_RS12055 at 2533.933 kb on + strand, within BWI76_RS12055
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,531,708 + BWI76_RS12040 0.14 -0.0 2,531,983 + -1.0 2,532,114 - BWI76_RS12045 0.20 -0.7 2,532,233 + BWI76_RS12045 0.36 -0.7 2,532,627 + +0.4 2,532,627 + -0.3 2,532,642 + -0.5 2,532,645 - -0.4 2,532,869 + BWI76_RS12050 0.53 -2.4 2,532,877 - BWI76_RS12050 0.55 -1.0 2,532,937 + BWI76_RS12050 0.72 -1.6 2,532,956 - -1.4 2,532,989 - BWI76_RS12050 0.86 -0.2 2,533,106 - +0.2 2,533,276 + BWI76_RS12055 0.18 +1.8 2,533,531 - BWI76_RS12055 0.41 -0.2 2,533,662 - BWI76_RS12055 0.53 +0.4 2,533,933 + BWI76_RS12055 0.78 -0.3
Or see this region's nucleotide sequence