Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10270 and BWI76_RS10275 are separated by 13 nucleotides BWI76_RS10275 and BWI76_RS10280 overlap by 4 nucleotides BWI76_RS10280 and BWI76_RS10285 are separated by 28 nucleotides
BWI76_RS10270: BWI76_RS10270 - acetylornithine deacetylase, at 2,175,145 to 2,176,320
_RS10270
BWI76_RS10275: BWI76_RS10275 - ABC transporter, at 2,176,334 to 2,177,074
_RS10275
BWI76_RS10280: BWI76_RS10280 - polar amino acid ABC transporter permease, at 2,177,071 to 2,177,796
_RS10280
BWI76_RS10285: BWI76_RS10285 - family 3 extracellular solute-binding protein, at 2,177,825 to 2,178,637
_RS10285
Position (kb)
2176
2177
2178 Strain fitness (log2 ratio)
-1
0
1 at 2175.825 kb on - strand, within BWI76_RS10270 at 2176.089 kb on - strand, within BWI76_RS10270 at 2176.116 kb on - strand, within BWI76_RS10270 at 2176.353 kb on + strand at 2176.494 kb on - strand, within BWI76_RS10275 at 2176.517 kb on - strand, within BWI76_RS10275 at 2176.813 kb on + strand, within BWI76_RS10275 at 2176.990 kb on + strand, within BWI76_RS10275 at 2177.183 kb on + strand, within BWI76_RS10280 at 2177.191 kb on - strand, within BWI76_RS10280 at 2177.663 kb on + strand, within BWI76_RS10280 at 2177.696 kb on + strand, within BWI76_RS10280 at 2177.701 kb on + strand, within BWI76_RS10280 at 2177.706 kb on + strand, within BWI76_RS10280 at 2177.793 kb on + strand at 2177.981 kb on - strand, within BWI76_RS10285
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,175,825 - BWI76_RS10270 0.58 +0.8 2,176,089 - BWI76_RS10270 0.80 +0.0 2,176,116 - BWI76_RS10270 0.83 +0.4 2,176,353 + -0.5 2,176,494 - BWI76_RS10275 0.22 +0.5 2,176,517 - BWI76_RS10275 0.25 +0.4 2,176,813 + BWI76_RS10275 0.65 +1.1 2,176,990 + BWI76_RS10275 0.89 +0.4 2,177,183 + BWI76_RS10280 0.15 -0.2 2,177,191 - BWI76_RS10280 0.17 +0.2 2,177,663 + BWI76_RS10280 0.82 +0.4 2,177,696 + BWI76_RS10280 0.86 +1.5 2,177,701 + BWI76_RS10280 0.87 -0.2 2,177,706 + BWI76_RS10280 0.87 -0.6 2,177,793 + -0.1 2,177,981 - BWI76_RS10285 0.19 -1.3
Or see this region's nucleotide sequence