Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10045 and BWI76_RS10050 are separated by 190 nucleotides BWI76_RS10050 and BWI76_RS10055 are separated by 15 nucleotides BWI76_RS10055 and BWI76_RS10060 are separated by 51 nucleotides BWI76_RS10060 and BWI76_RS10065 overlap by 4 nucleotides BWI76_RS10065 and BWI76_RS10070 are separated by 89 nucleotides
BWI76_RS10045: BWI76_RS10045 - 23S rRNA (cytosine(1962)-C(5))-methyltransferase, at 2,128,323 to 2,129,525
_RS10045
BWI76_RS10050: BWI76_RS10050 - outer membrane protein, at 2,129,716 to 2,130,270
_RS10050
BWI76_RS10055: BWI76_RS10055 - AraC family transcriptional regulator, at 2,130,286 to 2,131,074
_RS10055
BWI76_RS10060: BWI76_RS10060 - acylphosphatase, at 2,131,126 to 2,131,407
_RS10060
BWI76_RS10065: BWI76_RS10065 - sulfurtransferase TusE, at 2,131,404 to 2,131,733
_RS10065
BWI76_RS10070: BWI76_RS10070 - BAX inhibitor protein, at 2,131,823 to 2,132,482
_RS10070
Position (kb)
2130
2131
2132 Strain fitness (log2 ratio)
-1
0
1 at 2129.803 kb on + strand, within BWI76_RS10050 at 2129.811 kb on - strand, within BWI76_RS10050 at 2129.980 kb on + strand, within BWI76_RS10050 at 2130.069 kb on - strand, within BWI76_RS10050 at 2130.140 kb on + strand, within BWI76_RS10050 at 2130.164 kb on + strand, within BWI76_RS10050 at 2130.246 kb on + strand at 2130.784 kb on + strand, within BWI76_RS10055 at 2130.866 kb on + strand, within BWI76_RS10055 at 2131.088 kb on + strand at 2131.358 kb on + strand, within BWI76_RS10060
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,129,803 + BWI76_RS10050 0.16 +0.2 2,129,811 - BWI76_RS10050 0.17 +0.1 2,129,980 + BWI76_RS10050 0.48 -0.5 2,130,069 - BWI76_RS10050 0.64 +0.3 2,130,140 + BWI76_RS10050 0.76 +0.1 2,130,164 + BWI76_RS10050 0.81 +0.4 2,130,246 + -1.3 2,130,784 + BWI76_RS10055 0.63 -1.4 2,130,866 + BWI76_RS10055 0.74 -1.3 2,131,088 + +0.4 2,131,358 + BWI76_RS10060 0.82 -0.3
Or see this region's nucleotide sequence