Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09060 and BWI76_RS09065 are separated by 136 nucleotides BWI76_RS09065 and BWI76_RS09070 are separated by 209 nucleotides
BWI76_RS09060: BWI76_RS09060 - hypothetical protein, at 1,911,179 to 1,912,444
_RS09060
BWI76_RS09065: BWI76_RS09065 - sugar phosphatase SupH, at 1,912,581 to 1,913,396
_RS09065
BWI76_RS09070: BWI76_RS09070 - formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme, at 1,913,606 to 1,916,038
_RS09070
Position (kb)
1912
1913
1914 Strain fitness (log2 ratio)
-1
0
1 at 1911.667 kb on - strand, within BWI76_RS09060 at 1911.710 kb on - strand, within BWI76_RS09060 at 1912.334 kb on + strand at 1912.435 kb on - strand at 1912.570 kb on - strand at 1912.791 kb on - strand, within BWI76_RS09065 at 1912.817 kb on - strand, within BWI76_RS09065 at 1912.827 kb on - strand, within BWI76_RS09065 at 1913.000 kb on + strand, within BWI76_RS09065 at 1913.593 kb on - strand at 1913.657 kb on - strand at 1913.713 kb on + strand at 1914.238 kb on - strand, within BWI76_RS09070 at 1914.290 kb on - strand, within BWI76_RS09070 at 1914.308 kb on - strand, within BWI76_RS09070 at 1914.388 kb on + strand, within BWI76_RS09070 at 1914.388 kb on + strand, within BWI76_RS09070 at 1914.396 kb on - strand, within BWI76_RS09070 at 1914.396 kb on - strand, within BWI76_RS09070
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,911,667 - BWI76_RS09060 0.39 -0.2 1,911,710 - BWI76_RS09060 0.42 +0.1 1,912,334 + +0.2 1,912,435 - +0.1 1,912,570 - +0.9 1,912,791 - BWI76_RS09065 0.26 +0.6 1,912,817 - BWI76_RS09065 0.29 +0.5 1,912,827 - BWI76_RS09065 0.30 +0.0 1,913,000 + BWI76_RS09065 0.51 +1.4 1,913,593 - +0.0 1,913,657 - +0.3 1,913,713 + -1.6 1,914,238 - BWI76_RS09070 0.26 -0.2 1,914,290 - BWI76_RS09070 0.28 +0.6 1,914,308 - BWI76_RS09070 0.29 +0.1 1,914,388 + BWI76_RS09070 0.32 +0.3 1,914,388 + BWI76_RS09070 0.32 -0.5 1,914,396 - BWI76_RS09070 0.32 +0.2 1,914,396 - BWI76_RS09070 0.32 +1.0
Or see this region's nucleotide sequence