Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08760 and BWI76_RS08765 overlap by 4 nucleotides BWI76_RS08765 and BWI76_RS08770 overlap by 17 nucleotides BWI76_RS08770 and BWI76_RS08775 overlap by 8 nucleotides
BWI76_RS08760: BWI76_RS08760 - biotin synthase, at 1,846,413 to 1,847,453
_RS08760
BWI76_RS08765: BWI76_RS08765 - 8-amino-7-oxononanoate synthase, at 1,847,450 to 1,848,607
_RS08765
BWI76_RS08770: BWI76_RS08770 - malonyl-ACP O-methyltransferase BioC, at 1,848,591 to 1,849,346
_RS08770
BWI76_RS08775: BWI76_RS08775 - dethiobiotin synthase, at 1,849,339 to 1,850,061
_RS08775
Position (kb)
1847
1848
1849 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1846.536 kb on - strand, within BWI76_RS08760 at 1846.653 kb on + strand, within BWI76_RS08760 at 1846.747 kb on + strand, within BWI76_RS08760 at 1846.872 kb on + strand, within BWI76_RS08760 at 1846.937 kb on - strand, within BWI76_RS08760 at 1847.691 kb on + strand, within BWI76_RS08765 at 1847.826 kb on - strand, within BWI76_RS08765 at 1848.089 kb on + strand, within BWI76_RS08765 at 1848.463 kb on + strand, within BWI76_RS08765 at 1848.539 kb on - strand at 1848.691 kb on + strand, within BWI76_RS08770 at 1848.740 kb on + strand, within BWI76_RS08770 at 1848.968 kb on + strand, within BWI76_RS08770 at 1849.437 kb on - strand, within BWI76_RS08775 at 1849.551 kb on + strand, within BWI76_RS08775 at 1849.559 kb on - strand, within BWI76_RS08775
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,846,536 - BWI76_RS08760 0.12 +0.1 1,846,653 + BWI76_RS08760 0.23 +0.4 1,846,747 + BWI76_RS08760 0.32 -2.3 1,846,872 + BWI76_RS08760 0.44 +2.5 1,846,937 - BWI76_RS08760 0.50 +0.3 1,847,691 + BWI76_RS08765 0.21 +2.8 1,847,826 - BWI76_RS08765 0.32 +1.0 1,848,089 + BWI76_RS08765 0.55 +0.6 1,848,463 + BWI76_RS08765 0.87 +0.8 1,848,539 - -0.0 1,848,691 + BWI76_RS08770 0.13 +0.8 1,848,740 + BWI76_RS08770 0.20 +0.1 1,848,968 + BWI76_RS08770 0.50 +0.5 1,849,437 - BWI76_RS08775 0.14 -0.2 1,849,551 + BWI76_RS08775 0.29 -0.1 1,849,559 - BWI76_RS08775 0.30 +0.5
Or see this region's nucleotide sequence