Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08100

Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08095 and BWI76_RS08100 overlap by 1 nucleotidesBWI76_RS08100 and BWI76_RS08105 overlap by 1 nucleotidesBWI76_RS08105 and BWI76_RS08110 are separated by 163 nucleotides BWI76_RS08095: BWI76_RS08095 - arginine ABC transporter ATP-binding protein, at 1,716,367 to 1,717,092 _RS08095 BWI76_RS08100: BWI76_RS08100 - glutamate/aspartate transporter permease GltK, at 1,717,092 to 1,717,766 _RS08100 BWI76_RS08105: BWI76_RS08105 - glutamate/aspartate ABC transporter permease GltJ, at 1,717,766 to 1,718,506 _RS08105 BWI76_RS08110: BWI76_RS08110 - glutamate/aspartate ABC transporter substrate-binding protein, at 1,718,670 to 1,719,581 _RS08110 Position (kb) 1717 1718Strain fitness (log2 ratio) -3 -2 -1 0 1at 1716.179 kb on + strandat 1716.184 kb on + strandat 1716.184 kb on + strandat 1716.253 kb on + strandat 1716.260 kb on + strandat 1716.292 kb on - strandat 1716.339 kb on - strandat 1716.342 kb on - strandat 1716.372 kb on - strandat 1716.373 kb on - strandat 1716.373 kb on - strandat 1716.385 kb on - strandat 1716.396 kb on - strandat 1716.407 kb on + strandat 1716.407 kb on + strandat 1716.415 kb on - strandat 1716.460 kb on - strand, within BWI76_RS08095at 1716.462 kb on + strand, within BWI76_RS08095at 1716.530 kb on - strand, within BWI76_RS08095at 1716.537 kb on - strand, within BWI76_RS08095at 1716.590 kb on - strand, within BWI76_RS08095at 1716.599 kb on + strand, within BWI76_RS08095at 1716.626 kb on - strand, within BWI76_RS08095at 1716.638 kb on - strand, within BWI76_RS08095at 1716.677 kb on + strand, within BWI76_RS08095at 1716.685 kb on - strand, within BWI76_RS08095at 1716.779 kb on + strand, within BWI76_RS08095at 1716.780 kb on - strand, within BWI76_RS08095at 1716.787 kb on - strand, within BWI76_RS08095at 1716.787 kb on - strand, within BWI76_RS08095at 1716.787 kb on - strand, within BWI76_RS08095at 1716.839 kb on - strand, within BWI76_RS08095at 1716.927 kb on - strand, within BWI76_RS08095at 1716.976 kb on + strand, within BWI76_RS08095at 1717.097 kb on - strandat 1717.246 kb on + strand, within BWI76_RS08100at 1717.357 kb on + strand, within BWI76_RS08100at 1717.357 kb on + strand, within BWI76_RS08100at 1717.365 kb on - strand, within BWI76_RS08100at 1717.365 kb on - strand, within BWI76_RS08100at 1717.386 kb on - strand, within BWI76_RS08100at 1717.699 kb on + strandat 1717.856 kb on + strand, within BWI76_RS08105at 1717.883 kb on + strand, within BWI76_RS08105at 1717.906 kb on - strand, within BWI76_RS08105at 1718.024 kb on - strand, within BWI76_RS08105at 1718.034 kb on - strand, within BWI76_RS08105at 1718.122 kb on + strand, within BWI76_RS08105at 1718.210 kb on - strand, within BWI76_RS08105at 1718.244 kb on - strand, within BWI76_RS08105at 1718.333 kb on + strand, within BWI76_RS08105at 1718.416 kb on + strand, within BWI76_RS08105at 1718.703 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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1,716,179 + +0.6
1,716,184 + -0.9
1,716,184 + -1.4
1,716,253 + +0.5
1,716,260 + +0.3
1,716,292 - -1.4
1,716,339 - -0.0
1,716,342 - -1.0
1,716,372 - -0.8
1,716,373 - -0.0
1,716,373 - +0.5
1,716,385 - +1.1
1,716,396 - +0.8
1,716,407 + -1.0
1,716,407 + -0.8
1,716,415 - -0.1
1,716,460 - BWI76_RS08095 0.13 -2.5
1,716,462 + BWI76_RS08095 0.13 -0.4
1,716,530 - BWI76_RS08095 0.22 -0.1
1,716,537 - BWI76_RS08095 0.23 +1.2
1,716,590 - BWI76_RS08095 0.31 +1.0
1,716,599 + BWI76_RS08095 0.32 +1.0
1,716,626 - BWI76_RS08095 0.36 -1.7
1,716,638 - BWI76_RS08095 0.37 -0.1
1,716,677 + BWI76_RS08095 0.43 +0.4
1,716,685 - BWI76_RS08095 0.44 -0.4
1,716,779 + BWI76_RS08095 0.57 +1.0
1,716,780 - BWI76_RS08095 0.57 -0.5
1,716,787 - BWI76_RS08095 0.58 -0.5
1,716,787 - BWI76_RS08095 0.58 -0.0
1,716,787 - BWI76_RS08095 0.58 -0.2
1,716,839 - BWI76_RS08095 0.65 -3.0
1,716,927 - BWI76_RS08095 0.77 +0.0
1,716,976 + BWI76_RS08095 0.84 -1.4
1,717,097 - -0.4
1,717,246 + BWI76_RS08100 0.23 +0.4
1,717,357 + BWI76_RS08100 0.39 -1.0
1,717,357 + BWI76_RS08100 0.39 -0.5
1,717,365 - BWI76_RS08100 0.40 +0.4
1,717,365 - BWI76_RS08100 0.40 -0.4
1,717,386 - BWI76_RS08100 0.44 -0.7
1,717,699 + -1.4
1,717,856 + BWI76_RS08105 0.12 -0.0
1,717,883 + BWI76_RS08105 0.16 +0.0
1,717,906 - BWI76_RS08105 0.19 +0.5
1,718,024 - BWI76_RS08105 0.35 -0.5
1,718,034 - BWI76_RS08105 0.36 -0.6
1,718,122 + BWI76_RS08105 0.48 -0.1
1,718,210 - BWI76_RS08105 0.60 -0.7
1,718,244 - BWI76_RS08105 0.65 -0.3
1,718,333 + BWI76_RS08105 0.77 -0.6
1,718,416 + BWI76_RS08105 0.88 +1.0
1,718,703 - +0.3

Or see this region's nucleotide sequence