Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05545

Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05540 and BWI76_RS05545 are separated by 258 nucleotidesBWI76_RS05545 and BWI76_RS05550 are separated by 106 nucleotides BWI76_RS05540: BWI76_RS05540 - cytosol nonspecific dipeptidase, at 1,160,604 to 1,162,061 _RS05540 BWI76_RS05545: BWI76_RS05545 - xanthine phosphoribosyltransferase, at 1,162,320 to 1,162,778 _RS05545 BWI76_RS05550: BWI76_RS05550 - fermentation/respiration switch protein, at 1,162,885 to 1,164,129 _RS05550 Position (kb) 1162 1163Strain fitness (log2 ratio) -2 -1 0 1 2at 1161.387 kb on + strand, within BWI76_RS05540at 1161.387 kb on + strand, within BWI76_RS05540at 1161.387 kb on + strand, within BWI76_RS05540at 1161.395 kb on - strand, within BWI76_RS05540at 1161.395 kb on - strand, within BWI76_RS05540at 1161.395 kb on - strand, within BWI76_RS05540at 1161.535 kb on + strand, within BWI76_RS05540at 1161.537 kb on - strand, within BWI76_RS05540at 1161.826 kb on + strand, within BWI76_RS05540at 1161.948 kb on + strandat 1161.995 kb on + strandat 1162.151 kb on + strandat 1162.185 kb on + strandat 1162.193 kb on - strandat 1162.256 kb on - strandat 1162.302 kb on - strandat 1162.304 kb on + strandat 1162.312 kb on - strandat 1162.312 kb on - strandat 1162.314 kb on - strandat 1162.728 kb on - strand, within BWI76_RS05545at 1162.989 kb on - strandat 1163.186 kb on + strand, within BWI76_RS05550at 1163.257 kb on - strand, within BWI76_RS05550at 1163.370 kb on + strand, within BWI76_RS05550at 1163.542 kb on - strand, within BWI76_RS05550at 1163.577 kb on - strand, within BWI76_RS05550at 1163.607 kb on + strand, within BWI76_RS05550at 1163.768 kb on + strand, within BWI76_RS05550at 1163.776 kb on - strand, within BWI76_RS05550at 1163.776 kb on - strand, within BWI76_RS05550

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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1,161,387 + BWI76_RS05540 0.54 -0.9
1,161,387 + BWI76_RS05540 0.54 +1.9
1,161,387 + BWI76_RS05540 0.54 -0.3
1,161,395 - BWI76_RS05540 0.54 +0.2
1,161,395 - BWI76_RS05540 0.54 +0.6
1,161,395 - BWI76_RS05540 0.54 -0.9
1,161,535 + BWI76_RS05540 0.64 -0.1
1,161,537 - BWI76_RS05540 0.64 -0.0
1,161,826 + BWI76_RS05540 0.84 -1.0
1,161,948 + -0.8
1,161,995 + +0.7
1,162,151 + -1.5
1,162,185 + +0.5
1,162,193 - -2.1
1,162,256 - -0.5
1,162,302 - +1.1
1,162,304 + -0.4
1,162,312 - -0.3
1,162,312 - -0.2
1,162,314 - -0.9
1,162,728 - BWI76_RS05545 0.89 -1.5
1,162,989 - -0.3
1,163,186 + BWI76_RS05550 0.24 -1.7
1,163,257 - BWI76_RS05550 0.30 -0.5
1,163,370 + BWI76_RS05550 0.39 -0.8
1,163,542 - BWI76_RS05550 0.53 +0.2
1,163,577 - BWI76_RS05550 0.56 +0.1
1,163,607 + BWI76_RS05550 0.58 -0.1
1,163,768 + BWI76_RS05550 0.71 -1.3
1,163,776 - BWI76_RS05550 0.72 -1.2
1,163,776 - BWI76_RS05550 0.72 -0.5

Or see this region's nucleotide sequence