Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03840 and BWI76_RS03845 are separated by 70 nucleotides BWI76_RS03845 and BWI76_RS03850 are separated by 10 nucleotides BWI76_RS03850 and BWI76_RS03855 are separated by 136 nucleotides BWI76_RS03855 and BWI76_RS03860 are separated by 12 nucleotides
BWI76_RS03840: BWI76_RS03840 - 4-hydroxyphenylacetate permease, at 794,732 to 796,108
_RS03840
BWI76_RS03845: BWI76_RS03845 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase, at 796,179 to 796,971
_RS03845
BWI76_RS03850: BWI76_RS03850 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 796,982 to 797,785
_RS03850
BWI76_RS03855: BWI76_RS03855 - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 797,922 to 798,302
_RS03855
BWI76_RS03860: BWI76_RS03860 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 798,315 to 799,172
_RS03860
Position (kb) 796
797
798 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 796.106 kb on + strand at 796.161 kb on + strand at 796.299 kb on + strand, within BWI76_RS03845 at 796.307 kb on - strand, within BWI76_RS03845 at 796.401 kb on + strand, within BWI76_RS03845 at 796.553 kb on + strand, within BWI76_RS03845 at 796.780 kb on + strand, within BWI76_RS03845 at 796.949 kb on - strand at 797.175 kb on - strand, within BWI76_RS03850 at 797.241 kb on - strand, within BWI76_RS03850 at 797.440 kb on - strand, within BWI76_RS03850 at 797.544 kb on + strand, within BWI76_RS03850 at 797.552 kb on - strand at 797.769 kb on - strand at 797.849 kb on - strand at 798.231 kb on - strand, within BWI76_RS03855 at 798.313 kb on + strand at 798.343 kb on + strand at 798.496 kb on + strand, within BWI76_RS03860 at 798.504 kb on - strand, within BWI76_RS03860 at 798.732 kb on - strand, within BWI76_RS03860
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 796,106 + +0.2 796,161 + +0.5 796,299 + BWI76_RS03845 0.15 +0.0 796,307 - BWI76_RS03845 0.16 +0.7 796,401 + BWI76_RS03845 0.28 -0.7 796,553 + BWI76_RS03845 0.47 -0.6 796,780 + BWI76_RS03845 0.76 -0.4 796,949 - +1.1 797,175 - BWI76_RS03850 0.24 +0.5 797,241 - BWI76_RS03850 0.32 +3.1 797,440 - BWI76_RS03850 0.57 -0.2 797,544 + BWI76_RS03850 0.70 +0.9 797,552 - +1.2 797,769 - +0.8 797,849 - -0.4 798,231 - BWI76_RS03855 0.81 -0.6 798,313 + +0.6 798,343 + +0.1 798,496 + BWI76_RS03860 0.21 -2.7 798,504 - BWI76_RS03860 0.22 -0.8 798,732 - BWI76_RS03860 0.49 +0.1
Or see this region's nucleotide sequence