Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03390 and BWI76_RS03395 are separated by 165 nucleotides BWI76_RS03395 and BWI76_RS03400 are separated by 146 nucleotides BWI76_RS03400 and BWI76_RS03405 are separated by 289 nucleotides
BWI76_RS03390: BWI76_RS03390 - hypothetical protein, at 698,544 to 699,287
_RS03390
BWI76_RS03395: BWI76_RS03395 - glyoxalase family protein, at 699,453 to 699,887
_RS03395
BWI76_RS03400: BWI76_RS03400 - hypothetical protein, at 700,034 to 700,357
_RS03400
BWI76_RS03405: BWI76_RS03405 - DNA/RNA non-specific endonuclease, at 700,647 to 701,351
_RS03405
Position (kb) 700
701 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 699.054 kb on + strand, within BWI76_RS03390 at 699.054 kb on + strand, within BWI76_RS03390 at 699.071 kb on + strand, within BWI76_RS03390 at 699.072 kb on - strand, within BWI76_RS03390 at 699.082 kb on - strand, within BWI76_RS03390 at 699.082 kb on - strand, within BWI76_RS03390 at 699.082 kb on - strand, within BWI76_RS03390 at 699.088 kb on + strand, within BWI76_RS03390 at 699.102 kb on - strand, within BWI76_RS03390 at 699.120 kb on - strand, within BWI76_RS03390 at 699.125 kb on + strand, within BWI76_RS03390 at 699.133 kb on - strand, within BWI76_RS03390 at 699.186 kb on + strand, within BWI76_RS03390 at 699.186 kb on + strand, within BWI76_RS03390 at 699.194 kb on - strand, within BWI76_RS03390 at 699.280 kb on - strand at 699.343 kb on + strand at 699.382 kb on + strand at 699.382 kb on + strand at 699.382 kb on + strand at 699.382 kb on + strand at 699.385 kb on - strand at 699.385 kb on - strand at 699.390 kb on - strand at 699.390 kb on - strand at 699.392 kb on + strand at 699.394 kb on + strand at 699.394 kb on + strand at 699.400 kb on - strand at 699.400 kb on - strand at 699.402 kb on - strand at 699.402 kb on - strand at 699.404 kb on + strand at 699.404 kb on + strand at 699.405 kb on + strand at 699.405 kb on + strand at 699.405 kb on + strand at 699.412 kb on - strand at 699.417 kb on - strand at 699.419 kb on + strand at 699.427 kb on - strand at 699.429 kb on + strand at 699.432 kb on - strand at 699.435 kb on + strand at 699.436 kb on + strand at 699.443 kb on - strand at 699.443 kb on - strand at 699.443 kb on - strand at 699.444 kb on + strand at 699.444 kb on + strand at 699.452 kb on - strand at 699.452 kb on - strand at 699.458 kb on + strand at 699.458 kb on + strand at 699.460 kb on - strand at 699.466 kb on - strand at 699.476 kb on - strand at 699.476 kb on - strand at 699.479 kb on + strand at 699.479 kb on + strand at 699.481 kb on + strand at 699.481 kb on + strand at 699.484 kb on + strand at 699.487 kb on - strand at 699.489 kb on + strand at 699.497 kb on - strand, within BWI76_RS03395 at 699.497 kb on - strand, within BWI76_RS03395 at 699.503 kb on - strand, within BWI76_RS03395 at 699.512 kb on - strand, within BWI76_RS03395 at 699.515 kb on + strand, within BWI76_RS03395 at 699.553 kb on + strand, within BWI76_RS03395 at 699.561 kb on + strand, within BWI76_RS03395 at 699.579 kb on + strand, within BWI76_RS03395 at 699.601 kb on + strand, within BWI76_RS03395 at 699.609 kb on - strand, within BWI76_RS03395 at 699.609 kb on - strand, within BWI76_RS03395 at 699.634 kb on - strand, within BWI76_RS03395 at 699.675 kb on + strand, within BWI76_RS03395 at 699.682 kb on + strand, within BWI76_RS03395 at 699.687 kb on + strand, within BWI76_RS03395 at 699.690 kb on - strand, within BWI76_RS03395 at 699.695 kb on + strand, within BWI76_RS03395 at 699.717 kb on + strand, within BWI76_RS03395 at 699.747 kb on - strand, within BWI76_RS03395 at 699.747 kb on - strand, within BWI76_RS03395 at 699.770 kb on - strand, within BWI76_RS03395 at 699.770 kb on - strand, within BWI76_RS03395 at 699.774 kb on + strand, within BWI76_RS03395 at 699.838 kb on + strand, within BWI76_RS03395 at 699.846 kb on - strand at 699.850 kb on - strand at 699.850 kb on - strand at 699.850 kb on - strand at 699.850 kb on - strand at 699.880 kb on + strand at 699.884 kb on + strand at 699.892 kb on - strand at 699.926 kb on - strand at 699.935 kb on + strand at 699.952 kb on - strand at 699.961 kb on + strand at 699.966 kb on + strand at 699.969 kb on - strand at 699.969 kb on - strand at 699.971 kb on + strand at 699.971 kb on + strand at 699.971 kb on + strand at 699.974 kb on - strand at 699.974 kb on - strand at 699.982 kb on + strand at 699.982 kb on + strand at 699.998 kb on - strand at 700.026 kb on - strand at 700.031 kb on - strand at 700.032 kb on + strand at 700.032 kb on + strand at 700.040 kb on - strand at 700.040 kb on - strand at 700.040 kb on - strand at 700.058 kb on + strand at 700.073 kb on - strand, within BWI76_RS03400 at 700.107 kb on - strand, within BWI76_RS03400 at 700.107 kb on - strand, within BWI76_RS03400 at 700.112 kb on - strand, within BWI76_RS03400 at 700.132 kb on + strand, within BWI76_RS03400 at 700.153 kb on + strand, within BWI76_RS03400 at 700.158 kb on + strand, within BWI76_RS03400 at 700.166 kb on - strand, within BWI76_RS03400 at 700.168 kb on + strand at 700.171 kb on - strand, within BWI76_RS03400 at 700.186 kb on + strand, within BWI76_RS03400 at 700.186 kb on + strand, within BWI76_RS03400 at 700.194 kb on - strand, within BWI76_RS03400 at 700.194 kb on - strand, within BWI76_RS03400 at 700.194 kb on - strand, within BWI76_RS03400 at 700.205 kb on - strand, within BWI76_RS03400 at 700.213 kb on - strand, within BWI76_RS03400 at 700.227 kb on - strand, within BWI76_RS03400 at 700.227 kb on - strand, within BWI76_RS03400 at 700.235 kb on + strand, within BWI76_RS03400 at 700.259 kb on - strand, within BWI76_RS03400 at 700.263 kb on + strand, within BWI76_RS03400 at 700.269 kb on + strand, within BWI76_RS03400 at 700.277 kb on - strand, within BWI76_RS03400 at 700.283 kb on - strand, within BWI76_RS03400 at 700.283 kb on - strand, within BWI76_RS03400 at 700.292 kb on - strand, within BWI76_RS03400 at 700.315 kb on + strand, within BWI76_RS03400 at 700.323 kb on - strand, within BWI76_RS03400 at 700.323 kb on - strand, within BWI76_RS03400 at 700.333 kb on - strand at 700.347 kb on + strand at 700.347 kb on + strand at 700.347 kb on + strand at 700.347 kb on + strand at 700.352 kb on + strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.360 kb on - strand at 700.377 kb on + strand at 700.390 kb on - strand at 700.395 kb on + strand at 700.395 kb on + strand at 700.397 kb on + strand at 700.414 kb on + strand at 700.414 kb on + strand at 700.414 kb on + strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.424 kb on - strand at 700.432 kb on - strand at 700.437 kb on + strand at 700.438 kb on + strand at 700.445 kb on - strand at 700.446 kb on - strand at 700.446 kb on - strand at 700.472 kb on + strand at 700.503 kb on - strand at 700.535 kb on + strand at 700.593 kb on - strand at 700.595 kb on + strand at 700.628 kb on + strand at 700.629 kb on + strand at 700.635 kb on + strand at 700.636 kb on + strand at 700.636 kb on - strand at 700.641 kb on + strand at 700.644 kb on - strand at 700.656 kb on + strand at 700.657 kb on + strand at 700.659 kb on - strand at 700.664 kb on - strand at 700.671 kb on - strand at 700.675 kb on + strand at 700.695 kb on - strand at 700.701 kb on + strand at 700.709 kb on - strand at 700.709 kb on - strand at 700.716 kb on - strand at 700.718 kb on + strand, within BWI76_RS03405 at 700.718 kb on + strand, within BWI76_RS03405 at 700.718 kb on + strand, within BWI76_RS03405 at 700.718 kb on + strand, within BWI76_RS03405 at 700.722 kb on + strand, within BWI76_RS03405 at 700.726 kb on - strand, within BWI76_RS03405 at 700.726 kb on - strand, within BWI76_RS03405 at 700.736 kb on - strand, within BWI76_RS03405 at 700.749 kb on - strand, within BWI76_RS03405 at 700.763 kb on + strand, within BWI76_RS03405 at 700.771 kb on - strand, within BWI76_RS03405 at 700.844 kb on + strand, within BWI76_RS03405 at 700.887 kb on + strand, within BWI76_RS03405 at 700.900 kb on - strand, within BWI76_RS03405 at 700.929 kb on - strand, within BWI76_RS03405 at 701.005 kb on - strand, within BWI76_RS03405 at 701.042 kb on + strand, within BWI76_RS03405 at 701.086 kb on + strand, within BWI76_RS03405 at 701.212 kb on - strand, within BWI76_RS03405 at 701.212 kb on - strand, within BWI76_RS03405 at 701.224 kb on + strand, within BWI76_RS03405 at 701.237 kb on - strand, within BWI76_RS03405 at 701.237 kb on - strand, within BWI76_RS03405 at 701.242 kb on - strand, within BWI76_RS03405 at 701.272 kb on + strand, within BWI76_RS03405 at 701.280 kb on - strand, within BWI76_RS03405 at 701.297 kb on + strand at 701.298 kb on - strand at 701.305 kb on - strand at 701.306 kb on - strand at 701.308 kb on + strand at 701.318 kb on + strand at 701.326 kb on - strand at 701.349 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 699,054 + BWI76_RS03390 0.69 -0.8 699,054 + BWI76_RS03390 0.69 +0.5 699,071 + BWI76_RS03390 0.71 +0.2 699,072 - BWI76_RS03390 0.71 -0.7 699,082 - BWI76_RS03390 0.72 -2.4 699,082 - BWI76_RS03390 0.72 -1.1 699,082 - BWI76_RS03390 0.72 -1.6 699,088 + BWI76_RS03390 0.73 -1.1 699,102 - BWI76_RS03390 0.75 -1.4 699,120 - BWI76_RS03390 0.77 -0.2 699,125 + BWI76_RS03390 0.78 +0.2 699,133 - BWI76_RS03390 0.79 -2.5 699,186 + BWI76_RS03390 0.86 +0.5 699,186 + BWI76_RS03390 0.86 -0.1 699,194 - BWI76_RS03390 0.87 -0.1 699,280 - +1.2 699,343 + -0.8 699,382 + -1.1 699,382 + +1.0 699,382 + -1.4 699,382 + +0.8 699,385 - +0.2 699,385 - -1.2 699,390 - -1.3 699,390 - +0.3 699,392 + +1.2 699,394 + -0.6 699,394 + -0.1 699,400 - +0.3 699,400 - +0.0 699,402 - -1.2 699,402 - -0.2 699,404 + -0.1 699,404 + +1.2 699,405 + +0.7 699,405 + -1.1 699,405 + +0.3 699,412 - +0.4 699,417 - -0.4 699,419 + -0.2 699,427 - +0.4 699,429 + +0.2 699,432 - -0.9 699,435 + +0.2 699,436 + +1.2 699,443 - +0.5 699,443 - +0.4 699,443 - -0.0 699,444 + -2.5 699,444 + +1.5 699,452 - +0.6 699,452 - -0.1 699,458 + +0.6 699,458 + -0.6 699,460 - +0.4 699,466 - -0.1 699,476 - -0.2 699,476 - +0.6 699,479 + +0.6 699,479 + -1.3 699,481 + +0.3 699,481 + +0.7 699,484 + -1.3 699,487 - +0.1 699,489 + -1.8 699,497 - BWI76_RS03395 0.10 +0.7 699,497 - BWI76_RS03395 0.10 +0.3 699,503 - BWI76_RS03395 0.11 -0.1 699,512 - BWI76_RS03395 0.14 -0.1 699,515 + BWI76_RS03395 0.14 -2.0 699,553 + BWI76_RS03395 0.23 -0.1 699,561 + BWI76_RS03395 0.25 -1.3 699,579 + BWI76_RS03395 0.29 -0.8 699,601 + BWI76_RS03395 0.34 +0.8 699,609 - BWI76_RS03395 0.36 -0.5 699,609 - BWI76_RS03395 0.36 -0.5 699,634 - BWI76_RS03395 0.42 +0.7 699,675 + BWI76_RS03395 0.51 -0.1 699,682 + BWI76_RS03395 0.53 -0.2 699,687 + BWI76_RS03395 0.54 +0.5 699,690 - BWI76_RS03395 0.54 +0.6 699,695 + BWI76_RS03395 0.56 -0.3 699,717 + BWI76_RS03395 0.61 -0.6 699,747 - BWI76_RS03395 0.68 +1.1 699,747 - BWI76_RS03395 0.68 -0.1 699,770 - BWI76_RS03395 0.73 -0.8 699,770 - BWI76_RS03395 0.73 -0.0 699,774 + BWI76_RS03395 0.74 -0.6 699,838 + BWI76_RS03395 0.89 +0.3 699,846 - +0.1 699,850 - -0.1 699,850 - -0.2 699,850 - -1.6 699,850 - +0.4 699,880 + +0.2 699,884 + -0.4 699,892 - -0.5 699,926 - -0.4 699,935 + +0.7 699,952 - +0.4 699,961 + -0.1 699,966 + -0.1 699,969 - +0.7 699,969 - -0.2 699,971 + +0.2 699,971 + +0.2 699,971 + +0.0 699,974 - -0.8 699,974 - +1.8 699,982 + +1.8 699,982 + +0.5 699,998 - -0.1 700,026 - -0.3 700,031 - +0.3 700,032 + -0.8 700,032 + -1.6 700,040 - +0.3 700,040 - -0.3 700,040 - -0.3 700,058 + -1.0 700,073 - BWI76_RS03400 0.12 -3.3 700,107 - BWI76_RS03400 0.23 -0.9 700,107 - BWI76_RS03400 0.23 -1.8 700,112 - BWI76_RS03400 0.24 -1.4 700,132 + BWI76_RS03400 0.30 -2.7 700,153 + BWI76_RS03400 0.37 -1.0 700,158 + BWI76_RS03400 0.38 +0.6 700,166 - BWI76_RS03400 0.41 -0.2 700,168 + +1.2 700,171 - BWI76_RS03400 0.42 +0.4 700,186 + BWI76_RS03400 0.47 -0.3 700,186 + BWI76_RS03400 0.47 +0.7 700,194 - BWI76_RS03400 0.49 -0.2 700,194 - BWI76_RS03400 0.49 -1.7 700,194 - BWI76_RS03400 0.49 +0.4 700,205 - BWI76_RS03400 0.53 +3.4 700,213 - BWI76_RS03400 0.55 -0.2 700,227 - BWI76_RS03400 0.60 +1.2 700,227 - BWI76_RS03400 0.60 +0.3 700,235 + BWI76_RS03400 0.62 +0.0 700,259 - BWI76_RS03400 0.69 -0.4 700,263 + BWI76_RS03400 0.71 +0.4 700,269 + BWI76_RS03400 0.73 -0.5 700,277 - BWI76_RS03400 0.75 -1.3 700,283 - BWI76_RS03400 0.77 +0.3 700,283 - BWI76_RS03400 0.77 -0.3 700,292 - BWI76_RS03400 0.80 -0.2 700,315 + BWI76_RS03400 0.87 -2.9 700,323 - BWI76_RS03400 0.89 -0.8 700,323 - BWI76_RS03400 0.89 -1.8 700,333 - -0.3 700,347 + +1.2 700,347 + -0.4 700,347 + +0.6 700,347 + +0.1 700,352 + +0.3 700,355 - +1.1 700,355 - +0.2 700,355 - -0.4 700,355 - +1.1 700,355 - +1.6 700,360 - +0.0 700,377 + +0.2 700,390 - -0.2 700,395 + +0.6 700,395 + +1.2 700,397 + +0.8 700,414 + -1.1 700,414 + -2.0 700,414 + -0.0 700,422 - +0.4 700,422 - +1.6 700,422 - +0.6 700,422 - +0.1 700,422 - -2.1 700,422 - -2.7 700,422 - -3.2 700,422 - -3.2 700,422 - -0.1 700,422 - -2.9 700,422 - -0.4 700,424 - +0.4 700,432 - -0.4 700,437 + -2.0 700,438 + +1.2 700,445 - -3.3 700,446 - +1.2 700,446 - +0.7 700,472 + -1.8 700,503 - -1.7 700,535 + -1.5 700,593 - -2.3 700,595 + +0.6 700,628 + +0.7 700,629 + -0.0 700,635 + -0.3 700,636 + -1.0 700,636 - -1.4 700,641 + -0.6 700,644 - -0.6 700,656 + -2.3 700,657 + -0.3 700,659 - -0.8 700,664 - +0.1 700,671 - +1.2 700,675 + +0.6 700,695 - +0.6 700,701 + -0.7 700,709 - +0.4 700,709 - -0.5 700,716 - -0.2 700,718 + BWI76_RS03405 0.10 -0.6 700,718 + BWI76_RS03405 0.10 +0.3 700,718 + BWI76_RS03405 0.10 +1.2 700,718 + BWI76_RS03405 0.10 -0.7 700,722 + BWI76_RS03405 0.11 +0.3 700,726 - BWI76_RS03405 0.11 -0.5 700,726 - BWI76_RS03405 0.11 +0.6 700,736 - BWI76_RS03405 0.13 +1.0 700,749 - BWI76_RS03405 0.14 -0.2 700,763 + BWI76_RS03405 0.16 -0.1 700,771 - BWI76_RS03405 0.18 -3.7 700,844 + BWI76_RS03405 0.28 -0.2 700,887 + BWI76_RS03405 0.34 +0.1 700,900 - BWI76_RS03405 0.36 +1.2 700,929 - BWI76_RS03405 0.40 +0.0 701,005 - BWI76_RS03405 0.51 -0.1 701,042 + BWI76_RS03405 0.56 -0.1 701,086 + BWI76_RS03405 0.62 -0.1 701,212 - BWI76_RS03405 0.80 +0.2 701,212 - BWI76_RS03405 0.80 -0.0 701,224 + BWI76_RS03405 0.82 -1.4 701,237 - BWI76_RS03405 0.84 -0.9 701,237 - BWI76_RS03405 0.84 -0.9 701,242 - BWI76_RS03405 0.84 -0.1 701,272 + BWI76_RS03405 0.89 +0.7 701,280 - BWI76_RS03405 0.90 +0.5 701,297 + +0.4 701,298 - -1.6 701,305 - +0.2 701,306 - -0.8 701,308 + -0.7 701,318 + +0.2 701,326 - -1.8 701,349 - -0.2
Or see this region's nucleotide sequence