Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02615 and BWI76_RS02620 are separated by 364 nucleotides BWI76_RS02620 and BWI76_RS02625 are separated by 12 nucleotides BWI76_RS02625 and BWI76_RS02630 are separated by 9 nucleotides BWI76_RS02630 and BWI76_RS02635 are separated by 13 nucleotides
BWI76_RS02615: BWI76_RS02615 - L-ascorbate-6-phosphate lactonase, at 534,628 to 535,692
_RS02615
BWI76_RS02620: BWI76_RS02620 - PTS ascorbate transporter subunit IIC, at 536,057 to 537,457
_RS02620
BWI76_RS02625: BWI76_RS02625 - PTS ascorbate transporter subunit IIB, at 537,470 to 537,775
_RS02625
BWI76_RS02630: BWI76_RS02630 - PTS ascorbate transporter subunit IIA, at 537,785 to 538,252
_RS02630
BWI76_RS02635: BWI76_RS02635 - 3-keto-L-gulonate-6-phosphate decarboxylase, at 538,266 to 538,916
_RS02635
Position (kb) 536
537
538 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 535.131 kb on - strand, within BWI76_RS02615 at 535.134 kb on - strand, within BWI76_RS02615 at 535.134 kb on - strand, within BWI76_RS02615 at 535.156 kb on - strand, within BWI76_RS02615 at 535.240 kb on + strand, within BWI76_RS02615 at 535.241 kb on - strand, within BWI76_RS02615 at 535.291 kb on + strand, within BWI76_RS02615 at 535.299 kb on - strand at 535.415 kb on + strand, within BWI76_RS02615 at 535.527 kb on - strand, within BWI76_RS02615 at 535.602 kb on - strand at 535.799 kb on + strand at 536.082 kb on - strand at 536.098 kb on + strand at 536.106 kb on - strand at 536.145 kb on + strand at 536.153 kb on - strand at 536.216 kb on - strand, within BWI76_RS02620 at 536.216 kb on - strand, within BWI76_RS02620 at 536.245 kb on - strand, within BWI76_RS02620 at 536.275 kb on + strand, within BWI76_RS02620 at 536.283 kb on - strand, within BWI76_RS02620 at 536.344 kb on + strand, within BWI76_RS02620 at 536.354 kb on + strand, within BWI76_RS02620 at 536.368 kb on + strand at 536.370 kb on + strand, within BWI76_RS02620 at 536.376 kb on - strand, within BWI76_RS02620 at 536.389 kb on - strand, within BWI76_RS02620 at 536.406 kb on - strand, within BWI76_RS02620 at 536.533 kb on + strand, within BWI76_RS02620 at 536.568 kb on - strand, within BWI76_RS02620 at 536.712 kb on + strand, within BWI76_RS02620 at 536.827 kb on + strand, within BWI76_RS02620 at 536.836 kb on + strand, within BWI76_RS02620 at 536.844 kb on - strand, within BWI76_RS02620 at 536.856 kb on + strand, within BWI76_RS02620 at 536.905 kb on + strand, within BWI76_RS02620 at 537.035 kb on + strand, within BWI76_RS02620 at 537.115 kb on - strand, within BWI76_RS02620 at 537.241 kb on + strand, within BWI76_RS02620 at 537.331 kb on - strand at 537.376 kb on + strand at 537.397 kb on + strand at 537.487 kb on + strand at 537.491 kb on + strand at 537.529 kb on + strand, within BWI76_RS02625 at 537.583 kb on - strand, within BWI76_RS02625 at 537.590 kb on - strand, within BWI76_RS02625 at 537.644 kb on - strand, within BWI76_RS02625 at 537.824 kb on - strand at 537.868 kb on + strand, within BWI76_RS02630 at 537.908 kb on + strand, within BWI76_RS02630 at 537.941 kb on - strand, within BWI76_RS02630 at 538.020 kb on + strand, within BWI76_RS02630 at 538.154 kb on - strand, within BWI76_RS02630 at 538.156 kb on + strand, within BWI76_RS02630 at 538.168 kb on + strand, within BWI76_RS02630 at 538.334 kb on + strand, within BWI76_RS02635 at 538.342 kb on - strand, within BWI76_RS02635
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 535,131 - BWI76_RS02615 0.47 -1.9 535,134 - BWI76_RS02615 0.48 -1.4 535,134 - BWI76_RS02615 0.48 +0.3 535,156 - BWI76_RS02615 0.50 +0.1 535,240 + BWI76_RS02615 0.57 -2.4 535,241 - BWI76_RS02615 0.58 -0.7 535,291 + BWI76_RS02615 0.62 +0.7 535,299 - +1.2 535,415 + BWI76_RS02615 0.74 +0.7 535,527 - BWI76_RS02615 0.84 +0.4 535,602 - +0.9 535,799 + +0.1 536,082 - -1.3 536,098 + +0.7 536,106 - -1.0 536,145 + +0.2 536,153 - +0.2 536,216 - BWI76_RS02620 0.11 +2.2 536,216 - BWI76_RS02620 0.11 +0.1 536,245 - BWI76_RS02620 0.13 -0.8 536,275 + BWI76_RS02620 0.16 -1.1 536,283 - BWI76_RS02620 0.16 -0.4 536,344 + BWI76_RS02620 0.20 -0.0 536,354 + BWI76_RS02620 0.21 +0.3 536,368 + +1.2 536,370 + BWI76_RS02620 0.22 +0.1 536,376 - BWI76_RS02620 0.23 -0.1 536,389 - BWI76_RS02620 0.24 +3.8 536,406 - BWI76_RS02620 0.25 +0.6 536,533 + BWI76_RS02620 0.34 +0.5 536,568 - BWI76_RS02620 0.36 +0.2 536,712 + BWI76_RS02620 0.47 +2.2 536,827 + BWI76_RS02620 0.55 -0.8 536,836 + BWI76_RS02620 0.56 +0.2 536,844 - BWI76_RS02620 0.56 -0.3 536,856 + BWI76_RS02620 0.57 +0.2 536,905 + BWI76_RS02620 0.61 +0.8 537,035 + BWI76_RS02620 0.70 +0.4 537,115 - BWI76_RS02620 0.76 +1.3 537,241 + BWI76_RS02620 0.85 -0.1 537,331 - -0.8 537,376 + +0.8 537,397 + +0.6 537,487 + +0.1 537,491 + -3.1 537,529 + BWI76_RS02625 0.19 -0.1 537,583 - BWI76_RS02625 0.37 -0.2 537,590 - BWI76_RS02625 0.39 +0.9 537,644 - BWI76_RS02625 0.57 -0.6 537,824 - +2.2 537,868 + BWI76_RS02630 0.18 +0.6 537,908 + BWI76_RS02630 0.26 +0.2 537,941 - BWI76_RS02630 0.33 -0.4 538,020 + BWI76_RS02630 0.50 -1.8 538,154 - BWI76_RS02630 0.79 -0.8 538,156 + BWI76_RS02630 0.79 +1.0 538,168 + BWI76_RS02630 0.82 +0.5 538,334 + BWI76_RS02635 0.10 -1.7 538,342 - BWI76_RS02635 0.12 -0.6
Or see this region's nucleotide sequence