Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00075 and BWI76_RS00080 overlap by 4 nucleotides BWI76_RS00080 and BWI76_RS00085 overlap by 4 nucleotides BWI76_RS00085 and BWI76_RS00090 are separated by 169 nucleotides
BWI76_RS00075: BWI76_RS00075 - N-acetylmannosamine-6-phosphate 2-epimerase, at 15,020 to 15,709
_RS00075
BWI76_RS00080: BWI76_RS00080 - N-acetylmannosamine kinase, at 15,706 to 16,581
_RS00080
BWI76_RS00085: BWI76_RS00085 - hypothetical protein, at 16,578 to 17,042
_RS00085
BWI76_RS00090: BWI76_RS00090 - multidrug transporter subunit MdtL, at 17,212 to 18,393
_RS00090
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-2
-1
0
1 at 16.009 kb on + strand, within BWI76_RS00080 at 16.225 kb on + strand, within BWI76_RS00080 at 16.451 kb on + strand, within BWI76_RS00080 at 16.461 kb on + strand, within BWI76_RS00080 at 16.469 kb on - strand, within BWI76_RS00080 at 16.514 kb on - strand at 16.529 kb on - strand at 16.601 kb on - strand at 16.609 kb on + strand at 16.616 kb on + strand at 16.631 kb on + strand, within BWI76_RS00085 at 16.631 kb on + strand, within BWI76_RS00085 at 16.932 kb on - strand, within BWI76_RS00085 at 17.020 kb on - strand at 17.088 kb on - strand at 17.176 kb on + strand at 17.176 kb on + strand at 17.176 kb on + strand at 17.176 kb on + strand at 17.184 kb on - strand at 17.184 kb on - strand at 17.214 kb on - strand at 17.244 kb on + strand at 17.252 kb on - strand at 17.252 kb on - strand at 17.498 kb on - strand, within BWI76_RS00090 at 17.539 kb on + strand, within BWI76_RS00090 at 17.546 kb on + strand, within BWI76_RS00090 at 17.672 kb on + strand, within BWI76_RS00090 at 17.683 kb on + strand, within BWI76_RS00090 at 17.688 kb on + strand, within BWI76_RS00090 at 17.696 kb on - strand, within BWI76_RS00090 at 17.881 kb on - strand, within BWI76_RS00090 at 17.963 kb on + strand, within BWI76_RS00090 at 18.025 kb on - strand, within BWI76_RS00090
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 16,009 + BWI76_RS00080 0.35 +0.8 16,225 + BWI76_RS00080 0.59 -0.7 16,451 + BWI76_RS00080 0.85 -0.1 16,461 + BWI76_RS00080 0.86 -0.3 16,469 - BWI76_RS00080 0.87 -0.3 16,514 - +0.0 16,529 - +0.9 16,601 - -2.0 16,609 + +1.3 16,616 + +0.4 16,631 + BWI76_RS00085 0.11 +0.0 16,631 + BWI76_RS00085 0.11 +0.9 16,932 - BWI76_RS00085 0.76 +0.8 17,020 - -0.6 17,088 - +0.3 17,176 + +0.1 17,176 + -0.0 17,176 + +0.7 17,176 + +1.3 17,184 - +0.0 17,184 - -0.7 17,214 - -0.5 17,244 + +0.4 17,252 - +0.5 17,252 - +1.1 17,498 - BWI76_RS00090 0.24 -1.9 17,539 + BWI76_RS00090 0.28 +0.2 17,546 + BWI76_RS00090 0.28 -0.3 17,672 + BWI76_RS00090 0.39 -0.1 17,683 + BWI76_RS00090 0.40 +0.3 17,688 + BWI76_RS00090 0.40 +0.6 17,696 - BWI76_RS00090 0.41 -0.4 17,881 - BWI76_RS00090 0.57 +0.7 17,963 + BWI76_RS00090 0.64 +0.7 18,025 - BWI76_RS00090 0.69 +0.6
Or see this region's nucleotide sequence