Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22170 and BWI76_RS22175 are separated by 130 nucleotides BWI76_RS22175 and BWI76_RS22180 are separated by 70 nucleotides BWI76_RS22180 and BWI76_RS22185 are separated by 80 nucleotides BWI76_RS22185 and BWI76_RS22190 are separated by 203 nucleotides
BWI76_RS22170: BWI76_RS22170 - alanine--tRNA ligase, at 4,622,705 to 4,625,332
_RS22170
BWI76_RS22175: BWI76_RS22175 - recombination regulator RecX, at 4,625,463 to 4,625,963
_RS22175
BWI76_RS22180: BWI76_RS22180 - recombinase RecA, at 4,626,034 to 4,627,092
_RS22180
BWI76_RS22185: BWI76_RS22185 - hypothetical protein, at 4,627,173 to 4,627,670
_RS22185
BWI76_RS22190: BWI76_RS22190 - hypothetical protein, at 4,627,874 to 4,628,066
_RS22190
Position (kb) 4626
4627
4628 Strain fitness (log2 ratio) -1
0
1
2 at 4625.512 kb on + strand at 4625.514 kb on - strand, within BWI76_RS22175 at 4625.936 kb on - strand at 4627.279 kb on - strand, within BWI76_RS22185 at 4627.346 kb on - strand, within BWI76_RS22185 at 4627.587 kb on - strand, within BWI76_RS22185 at 4627.894 kb on - strand, within BWI76_RS22190 at 4627.901 kb on - strand, within BWI76_RS22190 at 4627.949 kb on - strand, within BWI76_RS22190 at 4627.967 kb on - strand, within BWI76_RS22190 at 4628.001 kb on + strand, within BWI76_RS22190 at 4628.001 kb on + strand, within BWI76_RS22190 at 4628.002 kb on - strand, within BWI76_RS22190 at 4628.002 kb on - strand, within BWI76_RS22190 at 4628.002 kb on - strand, within BWI76_RS22190 at 4628.009 kb on - strand, within BWI76_RS22190
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,625,512 + +0.1 4,625,514 - BWI76_RS22175 0.10 +0.4 4,625,936 - +0.7 4,627,279 - BWI76_RS22185 0.21 -0.2 4,627,346 - BWI76_RS22185 0.35 -1.0 4,627,587 - BWI76_RS22185 0.83 -0.1 4,627,894 - BWI76_RS22190 0.10 +0.5 4,627,901 - BWI76_RS22190 0.14 +1.2 4,627,949 - BWI76_RS22190 0.39 +0.6 4,627,967 - BWI76_RS22190 0.48 +0.5 4,628,001 + BWI76_RS22190 0.66 +0.9 4,628,001 + BWI76_RS22190 0.66 -0.9 4,628,002 - BWI76_RS22190 0.66 -0.6 4,628,002 - BWI76_RS22190 0.66 +1.2 4,628,002 - BWI76_RS22190 0.66 +0.8 4,628,009 - BWI76_RS22190 0.70 +2.5
Or see this region's nucleotide sequence