Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20135 and BWI76_RS20140 are separated by 157 nucleotides BWI76_RS20140 and BWI76_RS20145 overlap by 4 nucleotides BWI76_RS20145 and BWI76_RS20150 overlap by 4 nucleotides BWI76_RS20150 and BWI76_RS20155 are separated by 10 nucleotides
BWI76_RS20135: BWI76_RS20135 - NADH-quinone oxidoreductase subunit M, at 4,218,451 to 4,219,980
_RS20135
BWI76_RS20140: BWI76_RS20140 - NADH-quinone oxidoreductase subunit L, at 4,220,138 to 4,221,979
_RS20140
BWI76_RS20145: BWI76_RS20145 - NADH-quinone oxidoreductase subunit K, at 4,221,976 to 4,222,278
_RS20145
BWI76_RS20150: BWI76_RS20150 - NADH:ubiquinone oxidoreductase subunit J, at 4,222,275 to 4,222,829
_RS20150
BWI76_RS20155: BWI76_RS20155 - NADH-quinone oxidoreductase subunit I, at 4,222,840 to 4,223,382
_RS20155
Position (kb) 4220
4221
4222 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4219.526 kb on + strand, within BWI76_RS20135 at 4219.829 kb on - strand at 4220.091 kb on - strand at 4220.202 kb on - strand at 4220.369 kb on - strand, within BWI76_RS20140 at 4220.380 kb on - strand, within BWI76_RS20140 at 4220.702 kb on - strand, within BWI76_RS20140 at 4221.166 kb on + strand, within BWI76_RS20140 at 4221.174 kb on - strand, within BWI76_RS20140 at 4221.317 kb on + strand, within BWI76_RS20140 at 4221.504 kb on + strand, within BWI76_RS20140 at 4221.544 kb on + strand, within BWI76_RS20140 at 4222.044 kb on + strand, within BWI76_RS20145 at 4222.323 kb on + strand at 4222.604 kb on + strand, within BWI76_RS20150 at 4222.645 kb on - strand, within BWI76_RS20150 at 4222.756 kb on + strand, within BWI76_RS20150 at 4222.891 kb on + strand at 4222.899 kb on - strand, within BWI76_RS20155
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,219,526 + BWI76_RS20135 0.70 -0.9 4,219,829 - +0.6 4,220,091 - -0.1 4,220,202 - -0.7 4,220,369 - BWI76_RS20140 0.13 -0.1 4,220,380 - BWI76_RS20140 0.13 -1.7 4,220,702 - BWI76_RS20140 0.31 -2.2 4,221,166 + BWI76_RS20140 0.56 -1.8 4,221,174 - BWI76_RS20140 0.56 +0.0 4,221,317 + BWI76_RS20140 0.64 -0.2 4,221,504 + BWI76_RS20140 0.74 -2.9 4,221,544 + BWI76_RS20140 0.76 -1.0 4,222,044 + BWI76_RS20145 0.22 -0.1 4,222,323 + +0.1 4,222,604 + BWI76_RS20150 0.59 -0.9 4,222,645 - BWI76_RS20150 0.67 -0.1 4,222,756 + BWI76_RS20150 0.87 -1.8 4,222,891 + +1.1 4,222,899 - BWI76_RS20155 0.11 +1.0
Or see this region's nucleotide sequence