Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18155 and BWI76_RS18160 are separated by 37 nucleotides BWI76_RS18160 and BWI76_RS18165 are separated by 28 nucleotides BWI76_RS18165 and BWI76_RS18170 are separated by 1 nucleotides
BWI76_RS18155: BWI76_RS18155 - crossover junction endodeoxyribonuclease RuvC, at 3,773,122 to 3,773,643
_RS18155
BWI76_RS18160: BWI76_RS18160 - YebC/PmpR family DNA-binding transcriptional regulator, at 3,773,681 to 3,774,421
_RS18160
BWI76_RS18165: BWI76_RS18165 - NUDIX pyrophosphatase, at 3,774,450 to 3,774,893
_RS18165
BWI76_RS18170: BWI76_RS18170 - aspartate--tRNA ligase, at 3,774,895 to 3,776,682
_RS18170
Position (kb) 3774
3775 Strain fitness (log2 ratio) -2
-1
0
1 at 3774.045 kb on + strand, within BWI76_RS18160 at 3774.158 kb on - strand, within BWI76_RS18160 at 3774.396 kb on - strand at 3774.396 kb on - strand at 3774.501 kb on - strand, within BWI76_RS18165 at 3774.780 kb on + strand, within BWI76_RS18165
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,774,045 + BWI76_RS18160 0.49 +0.5 3,774,158 - BWI76_RS18160 0.64 +0.0 3,774,396 - -1.8 3,774,396 - -0.8 3,774,501 - BWI76_RS18165 0.11 -1.4 3,774,780 + BWI76_RS18165 0.74 -0.1
Or see this region's nucleotide sequence