Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16860 and BWI76_RS16865 are separated by 53 nucleotides BWI76_RS16865 and BWI76_RS16870 are separated by 145 nucleotides
BWI76_RS16860: BWI76_RS16860 - acyl-CoA dehydrogenase, at 3,496,352 to 3,497,440
_RS16860
BWI76_RS16865: BWI76_RS16865 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase-related flavin-dependent oxidoreductase, at 3,497,494 to 3,498,579
_RS16865
BWI76_RS16870: BWI76_RS16870 - LysR family transcriptional regulator, at 3,498,725 to 3,499,618
_RS16870
Position (kb)
3497
3498
3499 Strain fitness (log2 ratio)
-1
0
1 at 3496.666 kb on + strand, within BWI76_RS16860 at 3496.720 kb on - strand, within BWI76_RS16860 at 3497.655 kb on + strand, within BWI76_RS16865 at 3497.663 kb on + strand, within BWI76_RS16865 at 3497.663 kb on - strand, within BWI76_RS16865 at 3497.738 kb on + strand, within BWI76_RS16865 at 3498.029 kb on + strand, within BWI76_RS16865 at 3498.154 kb on + strand, within BWI76_RS16865 at 3498.173 kb on + strand at 3498.181 kb on - strand, within BWI76_RS16865 at 3498.647 kb on - strand at 3498.975 kb on + strand, within BWI76_RS16870 at 3498.976 kb on - strand, within BWI76_RS16870 at 3499.376 kb on - strand, within BWI76_RS16870
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,496,666 + BWI76_RS16860 0.29 -0.1 3,496,720 - BWI76_RS16860 0.34 -0.4 3,497,655 + BWI76_RS16865 0.15 +0.5 3,497,663 + BWI76_RS16865 0.16 -0.1 3,497,663 - BWI76_RS16865 0.16 +0.6 3,497,738 + BWI76_RS16865 0.22 -0.3 3,498,029 + BWI76_RS16865 0.49 +0.7 3,498,154 + BWI76_RS16865 0.61 +0.9 3,498,173 + +1.4 3,498,181 - BWI76_RS16865 0.63 +0.6 3,498,647 - +0.5 3,498,975 + BWI76_RS16870 0.28 +0.2 3,498,976 - BWI76_RS16870 0.28 -0.1 3,499,376 - BWI76_RS16870 0.73 +0.2
Or see this region's nucleotide sequence