Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13585

Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13575 and BWI76_RS13580 are separated by 100 nucleotidesBWI76_RS13580 and BWI76_RS13585 are separated by 68 nucleotidesBWI76_RS13585 and BWI76_RS13590 are separated by 246 nucleotidesBWI76_RS13590 and BWI76_RS13595 are separated by 75 nucleotides BWI76_RS13575: BWI76_RS13575 - sugar binding transcriptional regulator, LacI family protein, at 2,830,689 to 2,831,693 _RS13575 BWI76_RS13580: BWI76_RS13580 - glutathione S-transferase domain protein, at 2,831,794 to 2,832,411 _RS13580 BWI76_RS13585: BWI76_RS13585 - 4-oxalocrotonate tautomerase, at 2,832,480 to 2,832,707 _RS13585 BWI76_RS13590: BWI76_RS13590 - hypothetical protein, at 2,832,954 to 2,833,271 _RS13590 BWI76_RS13595: BWI76_RS13595 - transcriptional regulator, at 2,833,347 to 2,833,643 _RS13595 Position (kb) 2832 2833Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2831.722 kb on + strandat 2831.739 kb on + strandat 2832.196 kb on - strand, within BWI76_RS13580at 2832.609 kb on - strand, within BWI76_RS13585at 2832.621 kb on + strand, within BWI76_RS13585at 2832.631 kb on + strand, within BWI76_RS13585at 2832.707 kb on + strandat 2832.744 kb on + strandat 2833.122 kb on + strand, within BWI76_RS13590at 2833.127 kb on + strand, within BWI76_RS13590at 2833.216 kb on + strand, within BWI76_RS13590at 2833.333 kb on + strandat 2833.437 kb on + strand, within BWI76_RS13595at 2833.437 kb on + strand, within BWI76_RS13595at 2833.437 kb on + strand, within BWI76_RS13595at 2833.437 kb on + strand, within BWI76_RS13595at 2833.437 kb on + strand, within BWI76_RS13595at 2833.477 kb on + strand, within BWI76_RS13595at 2833.480 kb on + strand, within BWI76_RS13595at 2833.485 kb on + strand, within BWI76_RS13595at 2833.486 kb on + strand, within BWI76_RS13595at 2833.486 kb on + strand, within BWI76_RS13595at 2833.486 kb on + strand, within BWI76_RS13595at 2833.515 kb on + strand, within BWI76_RS13595at 2833.525 kb on + strand, within BWI76_RS13595at 2833.525 kb on + strand, within BWI76_RS13595at 2833.525 kb on + strand, within BWI76_RS13595at 2833.536 kb on + strand, within BWI76_RS13595at 2833.567 kb on + strand, within BWI76_RS13595at 2833.567 kb on + strand, within BWI76_RS13595at 2833.579 kb on + strand, within BWI76_RS13595at 2833.599 kb on + strand, within BWI76_RS13595at 2833.673 kb on + strandat 2833.673 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,831,722 + -0.1
2,831,739 + +0.9
2,832,196 - BWI76_RS13580 0.65 +0.8
2,832,609 - BWI76_RS13585 0.57 -1.8
2,832,621 + BWI76_RS13585 0.62 -1.9
2,832,631 + BWI76_RS13585 0.66 -1.1
2,832,707 + +0.4
2,832,744 + -1.1
2,833,122 + BWI76_RS13590 0.53 -0.1
2,833,127 + BWI76_RS13590 0.54 -0.2
2,833,216 + BWI76_RS13590 0.82 -1.5
2,833,333 + -0.1
2,833,437 + BWI76_RS13595 0.30 -1.1
2,833,437 + BWI76_RS13595 0.30 -4.1
2,833,437 + BWI76_RS13595 0.30 -1.7
2,833,437 + BWI76_RS13595 0.30 -1.5
2,833,437 + BWI76_RS13595 0.30 -4.8
2,833,477 + BWI76_RS13595 0.44 -0.7
2,833,480 + BWI76_RS13595 0.45 +0.4
2,833,485 + BWI76_RS13595 0.46 -0.2
2,833,486 + BWI76_RS13595 0.47 +0.3
2,833,486 + BWI76_RS13595 0.47 -0.2
2,833,486 + BWI76_RS13595 0.47 -0.8
2,833,515 + BWI76_RS13595 0.57 +0.5
2,833,525 + BWI76_RS13595 0.60 -0.6
2,833,525 + BWI76_RS13595 0.60 -0.4
2,833,525 + BWI76_RS13595 0.60 -0.2
2,833,536 + BWI76_RS13595 0.64 +0.5
2,833,567 + BWI76_RS13595 0.74 -5.8
2,833,567 + BWI76_RS13595 0.74 -0.1
2,833,579 + BWI76_RS13595 0.78 -1.3
2,833,599 + BWI76_RS13595 0.85 -4.3
2,833,673 + +0.9
2,833,673 + -1.3

Or see this region's nucleotide sequence