Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12285 and BWI76_RS12290 are separated by 1 nucleotides BWI76_RS12290 and BWI76_RS12295 overlap by 4 nucleotides BWI76_RS12295 and BWI76_RS12300 are separated by 22 nucleotides BWI76_RS12300 and BWI76_RS12305 are separated by 38 nucleotides BWI76_RS12305 and BWI76_RS12310 are separated by 137 nucleotides
BWI76_RS12285: BWI76_RS12285 - hypothetical protein, at 2,566,273 to 2,566,656
_RS12285
BWI76_RS12290: BWI76_RS12290 - hypothetical protein, at 2,566,658 to 2,567,209
_RS12290
BWI76_RS12295: BWI76_RS12295 - hypothetical protein, at 2,567,206 to 2,567,598
_RS12295
BWI76_RS12300: BWI76_RS12300 - hypothetical protein, at 2,567,621 to 2,568,553
_RS12300
BWI76_RS12305: BWI76_RS12305 - hypothetical protein, at 2,568,592 to 2,569,074
_RS12305
BWI76_RS12310: BWI76_RS12310 - hypothetical protein, at 2,569,212 to 2,569,427
_RS12310
Position (kb)
2567
2568
2569 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2566.641 kb on + strand at 2566.713 kb on + strand at 2566.768 kb on + strand, within BWI76_RS12290 at 2566.810 kb on - strand, within BWI76_RS12290 at 2566.941 kb on + strand, within BWI76_RS12290 at 2566.943 kb on + strand, within BWI76_RS12290 at 2567.027 kb on - strand, within BWI76_RS12290 at 2567.027 kb on - strand, within BWI76_RS12290 at 2567.121 kb on - strand, within BWI76_RS12290 at 2567.164 kb on + strand at 2567.198 kb on + strand at 2567.430 kb on + strand, within BWI76_RS12295 at 2567.470 kb on - strand, within BWI76_RS12295 at 2567.476 kb on + strand, within BWI76_RS12295 at 2567.501 kb on + strand, within BWI76_RS12295 at 2567.674 kb on - strand at 2567.724 kb on + strand, within BWI76_RS12300 at 2567.724 kb on + strand, within BWI76_RS12300 at 2567.732 kb on - strand, within BWI76_RS12300 at 2567.788 kb on - strand, within BWI76_RS12300 at 2567.953 kb on + strand, within BWI76_RS12300 at 2568.147 kb on + strand, within BWI76_RS12300 at 2568.147 kb on - strand, within BWI76_RS12300 at 2568.162 kb on + strand, within BWI76_RS12300 at 2568.271 kb on + strand, within BWI76_RS12300 at 2568.305 kb on + strand, within BWI76_RS12300 at 2568.381 kb on + strand, within BWI76_RS12300 at 2568.496 kb on + strand at 2568.496 kb on + strand at 2568.504 kb on - strand at 2568.553 kb on + strand at 2568.793 kb on + strand, within BWI76_RS12305 at 2568.816 kb on + strand, within BWI76_RS12305 at 2568.905 kb on + strand, within BWI76_RS12305 at 2568.926 kb on + strand, within BWI76_RS12305 at 2568.937 kb on + strand, within BWI76_RS12305 at 2569.001 kb on - strand, within BWI76_RS12305 at 2569.032 kb on + strand at 2569.040 kb on - strand at 2569.087 kb on + strand at 2569.432 kb on + strand at 2569.459 kb on + strand at 2569.459 kb on + strand at 2569.467 kb on - strand at 2569.467 kb on - strand at 2569.467 kb on - strand at 2569.485 kb on + strand at 2569.485 kb on - strand at 2569.490 kb on + strand at 2569.490 kb on + strand at 2569.490 kb on + strand at 2569.498 kb on - strand at 2569.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,566,641 + +0.2 2,566,713 + -2.7 2,566,768 + BWI76_RS12290 0.20 -0.5 2,566,810 - BWI76_RS12290 0.28 -0.4 2,566,941 + BWI76_RS12290 0.51 -0.6 2,566,943 + BWI76_RS12290 0.52 -1.3 2,567,027 - BWI76_RS12290 0.67 -1.0 2,567,027 - BWI76_RS12290 0.67 -0.8 2,567,121 - BWI76_RS12290 0.84 +0.7 2,567,164 + -0.7 2,567,198 + +1.7 2,567,430 + BWI76_RS12295 0.57 -0.3 2,567,470 - BWI76_RS12295 0.67 -1.2 2,567,476 + BWI76_RS12295 0.69 +0.6 2,567,501 + BWI76_RS12295 0.75 -1.0 2,567,674 - -1.6 2,567,724 + BWI76_RS12300 0.11 -1.9 2,567,724 + BWI76_RS12300 0.11 -2.7 2,567,732 - BWI76_RS12300 0.12 -0.7 2,567,788 - BWI76_RS12300 0.18 -2.3 2,567,953 + BWI76_RS12300 0.36 -0.6 2,568,147 + BWI76_RS12300 0.56 +0.2 2,568,147 - BWI76_RS12300 0.56 -1.1 2,568,162 + BWI76_RS12300 0.58 -0.7 2,568,271 + BWI76_RS12300 0.70 -2.1 2,568,305 + BWI76_RS12300 0.73 -2.6 2,568,381 + BWI76_RS12300 0.81 -1.3 2,568,496 + +0.2 2,568,496 + -0.9 2,568,504 - -0.3 2,568,553 + -0.4 2,568,793 + BWI76_RS12305 0.42 -0.7 2,568,816 + BWI76_RS12305 0.46 -0.2 2,568,905 + BWI76_RS12305 0.65 -1.6 2,568,926 + BWI76_RS12305 0.69 -0.7 2,568,937 + BWI76_RS12305 0.71 -2.3 2,569,001 - BWI76_RS12305 0.85 -1.1 2,569,032 + -1.2 2,569,040 - -1.3 2,569,087 + -0.6 2,569,432 + -0.1 2,569,459 + -0.5 2,569,459 + -2.5 2,569,467 - -2.8 2,569,467 - -2.0 2,569,467 - -1.8 2,569,485 + -0.3 2,569,485 - +3.3 2,569,490 + -0.9 2,569,490 + +0.1 2,569,490 + +0.3 2,569,498 - -2.5 2,569,498 - -1.4
Or see this region's nucleotide sequence