Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12300

Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12285 and BWI76_RS12290 are separated by 1 nucleotidesBWI76_RS12290 and BWI76_RS12295 overlap by 4 nucleotidesBWI76_RS12295 and BWI76_RS12300 are separated by 22 nucleotidesBWI76_RS12300 and BWI76_RS12305 are separated by 38 nucleotidesBWI76_RS12305 and BWI76_RS12310 are separated by 137 nucleotides BWI76_RS12285: BWI76_RS12285 - hypothetical protein, at 2,566,273 to 2,566,656 _RS12285 BWI76_RS12290: BWI76_RS12290 - hypothetical protein, at 2,566,658 to 2,567,209 _RS12290 BWI76_RS12295: BWI76_RS12295 - hypothetical protein, at 2,567,206 to 2,567,598 _RS12295 BWI76_RS12300: BWI76_RS12300 - hypothetical protein, at 2,567,621 to 2,568,553 _RS12300 BWI76_RS12305: BWI76_RS12305 - hypothetical protein, at 2,568,592 to 2,569,074 _RS12305 BWI76_RS12310: BWI76_RS12310 - hypothetical protein, at 2,569,212 to 2,569,427 _RS12310 Position (kb) 2567 2568 2569Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2566.641 kb on + strandat 2566.713 kb on + strandat 2566.768 kb on + strand, within BWI76_RS12290at 2566.810 kb on - strand, within BWI76_RS12290at 2566.941 kb on + strand, within BWI76_RS12290at 2566.943 kb on + strand, within BWI76_RS12290at 2567.027 kb on - strand, within BWI76_RS12290at 2567.027 kb on - strand, within BWI76_RS12290at 2567.121 kb on - strand, within BWI76_RS12290at 2567.164 kb on + strandat 2567.198 kb on + strandat 2567.430 kb on + strand, within BWI76_RS12295at 2567.470 kb on - strand, within BWI76_RS12295at 2567.476 kb on + strand, within BWI76_RS12295at 2567.501 kb on + strand, within BWI76_RS12295at 2567.674 kb on - strandat 2567.724 kb on + strand, within BWI76_RS12300at 2567.724 kb on + strand, within BWI76_RS12300at 2567.732 kb on - strand, within BWI76_RS12300at 2567.788 kb on - strand, within BWI76_RS12300at 2567.953 kb on + strand, within BWI76_RS12300at 2568.147 kb on + strand, within BWI76_RS12300at 2568.147 kb on - strand, within BWI76_RS12300at 2568.162 kb on + strand, within BWI76_RS12300at 2568.271 kb on + strand, within BWI76_RS12300at 2568.305 kb on + strand, within BWI76_RS12300at 2568.381 kb on + strand, within BWI76_RS12300at 2568.496 kb on + strandat 2568.496 kb on + strandat 2568.504 kb on - strandat 2568.553 kb on + strandat 2568.793 kb on + strand, within BWI76_RS12305at 2568.816 kb on + strand, within BWI76_RS12305at 2568.905 kb on + strand, within BWI76_RS12305at 2568.926 kb on + strand, within BWI76_RS12305at 2568.937 kb on + strand, within BWI76_RS12305at 2569.001 kb on - strand, within BWI76_RS12305at 2569.032 kb on + strandat 2569.040 kb on - strandat 2569.087 kb on + strandat 2569.432 kb on + strandat 2569.459 kb on + strandat 2569.459 kb on + strandat 2569.467 kb on - strandat 2569.467 kb on - strandat 2569.467 kb on - strandat 2569.485 kb on + strandat 2569.485 kb on - strandat 2569.490 kb on + strandat 2569.490 kb on + strandat 2569.490 kb on + strandat 2569.498 kb on - strandat 2569.498 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,566,641 + +0.2
2,566,713 + -2.7
2,566,768 + BWI76_RS12290 0.20 -0.5
2,566,810 - BWI76_RS12290 0.28 -0.4
2,566,941 + BWI76_RS12290 0.51 -0.6
2,566,943 + BWI76_RS12290 0.52 -1.3
2,567,027 - BWI76_RS12290 0.67 -1.0
2,567,027 - BWI76_RS12290 0.67 -0.8
2,567,121 - BWI76_RS12290 0.84 +0.7
2,567,164 + -0.7
2,567,198 + +1.7
2,567,430 + BWI76_RS12295 0.57 -0.3
2,567,470 - BWI76_RS12295 0.67 -1.2
2,567,476 + BWI76_RS12295 0.69 +0.6
2,567,501 + BWI76_RS12295 0.75 -1.0
2,567,674 - -1.6
2,567,724 + BWI76_RS12300 0.11 -1.9
2,567,724 + BWI76_RS12300 0.11 -2.7
2,567,732 - BWI76_RS12300 0.12 -0.7
2,567,788 - BWI76_RS12300 0.18 -2.3
2,567,953 + BWI76_RS12300 0.36 -0.6
2,568,147 + BWI76_RS12300 0.56 +0.2
2,568,147 - BWI76_RS12300 0.56 -1.1
2,568,162 + BWI76_RS12300 0.58 -0.7
2,568,271 + BWI76_RS12300 0.70 -2.1
2,568,305 + BWI76_RS12300 0.73 -2.6
2,568,381 + BWI76_RS12300 0.81 -1.3
2,568,496 + +0.2
2,568,496 + -0.9
2,568,504 - -0.3
2,568,553 + -0.4
2,568,793 + BWI76_RS12305 0.42 -0.7
2,568,816 + BWI76_RS12305 0.46 -0.2
2,568,905 + BWI76_RS12305 0.65 -1.6
2,568,926 + BWI76_RS12305 0.69 -0.7
2,568,937 + BWI76_RS12305 0.71 -2.3
2,569,001 - BWI76_RS12305 0.85 -1.1
2,569,032 + -1.2
2,569,040 - -1.3
2,569,087 + -0.6
2,569,432 + -0.1
2,569,459 + -0.5
2,569,459 + -2.5
2,569,467 - -2.8
2,569,467 - -2.0
2,569,467 - -1.8
2,569,485 + -0.3
2,569,485 - +3.3
2,569,490 + -0.9
2,569,490 + +0.1
2,569,490 + +0.3
2,569,498 - -2.5
2,569,498 - -1.4

Or see this region's nucleotide sequence