Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00980 and BWI76_RS00985 are separated by 58 nucleotides BWI76_RS00985 and BWI76_RS00990 are separated by 163 nucleotides
BWI76_RS00980: BWI76_RS00980 - PLP-dependent threonine dehydratase, at 200,147 to 201,691
_RS00980
BWI76_RS00985: BWI76_RS00985 - transcriptional regulator IlvY, at 201,750 to 202,640
_RS00985
BWI76_RS00990: BWI76_RS00990 - ketol-acid reductoisomerase, at 202,804 to 204,279
_RS00990
Position (kb) 201
202
203 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 200.792 kb on + strand, within BWI76_RS00980 at 200.845 kb on - strand, within BWI76_RS00980 at 200.845 kb on - strand, within BWI76_RS00980 at 200.869 kb on - strand, within BWI76_RS00980 at 200.929 kb on + strand, within BWI76_RS00980 at 201.380 kb on - strand, within BWI76_RS00980 at 201.454 kb on - strand at 201.505 kb on + strand, within BWI76_RS00980 at 201.565 kb on - strand at 201.629 kb on - strand at 201.918 kb on - strand, within BWI76_RS00985 at 201.928 kb on - strand, within BWI76_RS00985 at 201.974 kb on + strand, within BWI76_RS00985 at 202.030 kb on - strand, within BWI76_RS00985 at 202.043 kb on - strand, within BWI76_RS00985 at 202.101 kb on - strand, within BWI76_RS00985 at 202.114 kb on + strand, within BWI76_RS00985 at 202.114 kb on + strand, within BWI76_RS00985 at 202.177 kb on + strand, within BWI76_RS00985 at 202.264 kb on + strand, within BWI76_RS00985 at 202.444 kb on + strand, within BWI76_RS00985 at 202.515 kb on + strand, within BWI76_RS00985 at 202.560 kb on + strand at 202.610 kb on + strand at 202.625 kb on - strand at 202.796 kb on + strand at 202.862 kb on - strand at 202.960 kb on + strand, within BWI76_RS00990 at 202.968 kb on - strand, within BWI76_RS00990 at 202.971 kb on + strand, within BWI76_RS00990 at 202.976 kb on + strand, within BWI76_RS00990 at 203.048 kb on - strand, within BWI76_RS00990 at 203.182 kb on + strand, within BWI76_RS00990 at 203.379 kb on + strand, within BWI76_RS00990 at 203.617 kb on - strand, within BWI76_RS00990 at 203.617 kb on - strand, within BWI76_RS00990
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 200,792 + BWI76_RS00980 0.42 -2.1 200,845 - BWI76_RS00980 0.45 +1.7 200,845 - BWI76_RS00980 0.45 -2.2 200,869 - BWI76_RS00980 0.47 -2.3 200,929 + BWI76_RS00980 0.51 -4.0 201,380 - BWI76_RS00980 0.80 -0.9 201,454 - +0.7 201,505 + BWI76_RS00980 0.88 -1.6 201,565 - -1.9 201,629 - -0.9 201,918 - BWI76_RS00985 0.19 +0.3 201,928 - BWI76_RS00985 0.20 -2.7 201,974 + BWI76_RS00985 0.25 -0.6 202,030 - BWI76_RS00985 0.31 -3.0 202,043 - BWI76_RS00985 0.33 -2.3 202,101 - BWI76_RS00985 0.39 -2.9 202,114 + BWI76_RS00985 0.41 -3.3 202,114 + BWI76_RS00985 0.41 -1.8 202,177 + BWI76_RS00985 0.48 -1.2 202,264 + BWI76_RS00985 0.58 -1.4 202,444 + BWI76_RS00985 0.78 -1.7 202,515 + BWI76_RS00985 0.86 -0.6 202,560 + -0.9 202,610 + +0.7 202,625 - -1.1 202,796 + -1.3 202,862 - -4.3 202,960 + BWI76_RS00990 0.11 -0.3 202,968 - BWI76_RS00990 0.11 -3.9 202,971 + BWI76_RS00990 0.11 -2.3 202,976 + BWI76_RS00990 0.12 -5.2 203,048 - BWI76_RS00990 0.17 -2.5 203,182 + BWI76_RS00990 0.26 -4.5 203,379 + BWI76_RS00990 0.39 -1.6 203,617 - BWI76_RS00990 0.55 +0.7 203,617 - BWI76_RS00990 0.55 -3.1
Or see this region's nucleotide sequence