Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23330 and BWI76_RS23335 overlap by 40 nucleotides BWI76_RS23335 and BWI76_RS23340 overlap by 4 nucleotides BWI76_RS23340 and BWI76_RS23345 are separated by 68 nucleotides
BWI76_RS23330: BWI76_RS23330 - transcriptional regulator GalR, at 4,864,840 to 4,865,856
_RS23330
BWI76_RS23335: BWI76_RS23335 - GNAT family acetyltransferase, at 4,865,817 to 4,866,296
_RS23335
BWI76_RS23340: BWI76_RS23340 - transcriptional regulator, at 4,866,293 to 4,867,066
_RS23340
BWI76_RS23345: BWI76_RS23345 - glycoside hydrolase, at 4,867,135 to 4,868,562
_RS23345
Position (kb) 4866
4867
4868 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4865.306 kb on - strand, within BWI76_RS23330 at 4865.395 kb on - strand, within BWI76_RS23330 at 4865.440 kb on + strand, within BWI76_RS23330 at 4865.517 kb on + strand, within BWI76_RS23330 at 4865.962 kb on + strand, within BWI76_RS23335 at 4865.970 kb on - strand, within BWI76_RS23335 at 4865.970 kb on - strand, within BWI76_RS23335 at 4866.007 kb on - strand, within BWI76_RS23335 at 4866.161 kb on + strand, within BWI76_RS23335 at 4866.251 kb on + strand at 4866.274 kb on + strand at 4866.283 kb on + strand at 4866.296 kb on + strand at 4866.597 kb on + strand, within BWI76_RS23340 at 4866.858 kb on - strand, within BWI76_RS23340 at 4867.718 kb on + strand, within BWI76_RS23345 at 4868.058 kb on + strand, within BWI76_RS23345
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,865,306 - BWI76_RS23330 0.46 -2.1 4,865,395 - BWI76_RS23330 0.55 -0.6 4,865,440 + BWI76_RS23330 0.59 +0.1 4,865,517 + BWI76_RS23330 0.67 +0.1 4,865,962 + BWI76_RS23335 0.30 +0.2 4,865,970 - BWI76_RS23335 0.32 -1.7 4,865,970 - BWI76_RS23335 0.32 +1.9 4,866,007 - BWI76_RS23335 0.40 +0.3 4,866,161 + BWI76_RS23335 0.72 +0.5 4,866,251 + -0.0 4,866,274 + -1.1 4,866,283 + +0.2 4,866,296 + -1.0 4,866,597 + BWI76_RS23340 0.39 +1.0 4,866,858 - BWI76_RS23340 0.73 +0.5 4,867,718 + BWI76_RS23345 0.41 +0.2 4,868,058 + BWI76_RS23345 0.65 -0.6
Or see this region's nucleotide sequence