Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20960 and BWI76_RS20965 are separated by 265 nucleotides BWI76_RS20965 and BWI76_RS20970 overlap by 4 nucleotides BWI76_RS20970 and BWI76_RS20975 are separated by 43 nucleotides
BWI76_RS20960: BWI76_RS20960 - ROK family protein, at 4,381,211 to 4,382,104
_RS20960
BWI76_RS20965: BWI76_RS20965 - phosphoribosylformylglycinamidine cyclo-ligase, at 4,382,370 to 4,383,407
_RS20965
BWI76_RS20970: BWI76_RS20970 - phosphoribosylglycinamide formyltransferase, at 4,383,404 to 4,384,045
_RS20970
BWI76_RS20975: BWI76_RS20975 - hypothetical protein, at 4,384,089 to 4,385,447
_RS20975
Position (kb) 4382
4383
4384 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4381.419 kb on + strand, within BWI76_RS20960 at 4381.427 kb on - strand, within BWI76_RS20960 at 4381.475 kb on - strand, within BWI76_RS20960 at 4381.839 kb on - strand, within BWI76_RS20960 at 4381.839 kb on - strand, within BWI76_RS20960 at 4382.355 kb on + strand at 4382.628 kb on - strand, within BWI76_RS20965 at 4382.639 kb on - strand, within BWI76_RS20965 at 4383.163 kb on - strand, within BWI76_RS20965 at 4383.337 kb on - strand at 4383.346 kb on - strand at 4383.379 kb on + strand at 4383.431 kb on + strand at 4383.859 kb on + strand, within BWI76_RS20970 at 4384.255 kb on + strand, within BWI76_RS20975
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,381,419 + BWI76_RS20960 0.23 +0.7 4,381,427 - BWI76_RS20960 0.24 +0.8 4,381,475 - BWI76_RS20960 0.30 +2.0 4,381,839 - BWI76_RS20960 0.70 -0.4 4,381,839 - BWI76_RS20960 0.70 +0.8 4,382,355 + -1.5 4,382,628 - BWI76_RS20965 0.25 -1.8 4,382,639 - BWI76_RS20965 0.26 -1.9 4,383,163 - BWI76_RS20965 0.76 -2.8 4,383,337 - +1.0 4,383,346 - -0.5 4,383,379 + -1.9 4,383,431 + -0.6 4,383,859 + BWI76_RS20970 0.71 -0.3 4,384,255 + BWI76_RS20975 0.12 -1.4
Or see this region's nucleotide sequence