Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20765

Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20755 and BWI76_RS20760 are separated by 9 nucleotidesBWI76_RS20760 and BWI76_RS20765 are separated by 20 nucleotidesBWI76_RS20765 and BWI76_RS20770 are separated by 11 nucleotides BWI76_RS20755: BWI76_RS20755 - microcompartment protein EutL, at 4,338,114 to 4,338,773 _RS20755 BWI76_RS20760: BWI76_RS20760 - ethanolamine ammonia-lyase small subunit, at 4,338,783 to 4,339,676 _RS20760 BWI76_RS20765: BWI76_RS20765 - ethanolamine ammonia lyase large subunit, at 4,339,697 to 4,341,058 _RS20765 BWI76_RS20770: BWI76_RS20770 - reactivating factor for ethanolamine ammonia lyase, at 4,341,070 to 4,342,473 _RS20770 Position (kb) 4339 4340 4341 4342Strain fitness (log2 ratio) -1 0 1 2at 4338.714 kb on + strandat 4339.259 kb on - strand, within BWI76_RS20760at 4339.278 kb on - strand, within BWI76_RS20760at 4339.440 kb on - strand, within BWI76_RS20760at 4339.558 kb on + strand, within BWI76_RS20760at 4339.843 kb on + strand, within BWI76_RS20765at 4339.957 kb on + strand, within BWI76_RS20765at 4340.401 kb on - strand, within BWI76_RS20765at 4340.450 kb on + strand, within BWI76_RS20765at 4340.585 kb on + strand, within BWI76_RS20765at 4340.649 kb on + strand, within BWI76_RS20765at 4340.746 kb on + strand, within BWI76_RS20765at 4340.754 kb on - strand, within BWI76_RS20765at 4341.387 kb on + strand, within BWI76_RS20770at 4341.463 kb on + strand, within BWI76_RS20770at 4341.646 kb on - strand, within BWI76_RS20770at 4341.693 kb on + strand, within BWI76_RS20770at 4341.907 kb on - strand, within BWI76_RS20770at 4341.988 kb on - strand, within BWI76_RS20770

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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4,338,714 + +0.8
4,339,259 - BWI76_RS20760 0.53 +1.3
4,339,278 - BWI76_RS20760 0.55 +0.8
4,339,440 - BWI76_RS20760 0.73 +0.5
4,339,558 + BWI76_RS20760 0.87 +0.1
4,339,843 + BWI76_RS20765 0.11 +1.2
4,339,957 + BWI76_RS20765 0.19 +0.2
4,340,401 - BWI76_RS20765 0.52 +2.4
4,340,450 + BWI76_RS20765 0.55 +2.2
4,340,585 + BWI76_RS20765 0.65 +1.0
4,340,649 + BWI76_RS20765 0.70 -0.6
4,340,746 + BWI76_RS20765 0.77 +0.3
4,340,754 - BWI76_RS20765 0.78 -0.7
4,341,387 + BWI76_RS20770 0.23 +0.3
4,341,463 + BWI76_RS20770 0.28 +0.2
4,341,646 - BWI76_RS20770 0.41 +1.9
4,341,693 + BWI76_RS20770 0.44 +0.8
4,341,907 - BWI76_RS20770 0.60 +0.8
4,341,988 - BWI76_RS20770 0.65 +0.1

Or see this region's nucleotide sequence