Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20165 and BWI76_RS20170 are separated by 32 nucleotides BWI76_RS20170 and BWI76_RS20175 overlap by 4 nucleotides BWI76_RS20175 and BWI76_RS20180 are separated by 2 nucleotides
BWI76_RS20165: BWI76_RS20165 - NADH-quinone oxidoreductase subunit G, at 4,224,371 to 4,227,097
_RS20165
BWI76_RS20170: BWI76_RS20170 - NADH-quinone oxidoreductase subunit F, at 4,227,130 to 4,228,467
_RS20170
BWI76_RS20175: BWI76_RS20175 - NADH-quinone oxidoreductase subunit E, at 4,228,464 to 4,228,964
_RS20175
BWI76_RS20180: BWI76_RS20180 - NADH-quinone oxidoreductase subunit C/D, at 4,228,967 to 4,230,763
_RS20180
Position (kb)
4227
4228
4229 Strain fitness (log2 ratio)
-2
-1
0
1 at 4226.418 kb on - strand, within BWI76_RS20165 at 4226.753 kb on - strand, within BWI76_RS20165 at 4226.753 kb on - strand, within BWI76_RS20165 at 4226.908 kb on - strand at 4227.802 kb on - strand, within BWI76_RS20170 at 4227.826 kb on + strand, within BWI76_RS20170 at 4227.944 kb on + strand, within BWI76_RS20170 at 4228.008 kb on - strand, within BWI76_RS20170 at 4228.295 kb on - strand, within BWI76_RS20170 at 4228.438 kb on + strand at 4228.521 kb on - strand, within BWI76_RS20175
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,226,418 - BWI76_RS20165 0.75 -0.8 4,226,753 - BWI76_RS20165 0.87 +0.6 4,226,753 - BWI76_RS20165 0.87 +0.2 4,226,908 - -1.3 4,227,802 - BWI76_RS20170 0.50 -2.2 4,227,826 + BWI76_RS20170 0.52 -1.2 4,227,944 + BWI76_RS20170 0.61 +1.6 4,228,008 - BWI76_RS20170 0.66 -1.6 4,228,295 - BWI76_RS20170 0.87 -0.7 4,228,438 + +0.4 4,228,521 - BWI76_RS20175 0.11 +0.8
Or see this region's nucleotide sequence