Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15190 and BWI76_RS15195 are separated by 107 nucleotides BWI76_RS15195 and BWI76_RS15200 overlap by 1 nucleotides BWI76_RS15200 and BWI76_RS15205 are separated by 204 nucleotides
BWI76_RS15190: BWI76_RS15190 - GGDEF domain-containing protein, at 3,184,118 to 3,185,530
_RS15190
BWI76_RS15195: BWI76_RS15195 - DUF4186 domain-containing protein, at 3,185,638 to 3,186,012
_RS15195
BWI76_RS15200: BWI76_RS15200 - glutaminase, at 3,186,012 to 3,186,938
_RS15200
BWI76_RS15205: BWI76_RS15205 - succinate-semialdehyde dehydrogenase, at 3,187,143 to 3,188,528
_RS15205
Position (kb)
3186
3187 Strain fitness (log2 ratio)
-2
-1
0
1 at 3185.286 kb on - strand, within BWI76_RS15190 at 3185.294 kb on - strand, within BWI76_RS15190 at 3185.446 kb on + strand at 3185.446 kb on + strand at 3185.543 kb on + strand at 3185.710 kb on - strand, within BWI76_RS15195 at 3185.881 kb on - strand, within BWI76_RS15195 at 3186.093 kb on - strand at 3186.326 kb on + strand, within BWI76_RS15200 at 3186.539 kb on - strand, within BWI76_RS15200 at 3187.198 kb on - strand at 3187.473 kb on + strand, within BWI76_RS15205 at 3187.555 kb on - strand, within BWI76_RS15205
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,185,286 - BWI76_RS15190 0.83 +0.9 3,185,294 - BWI76_RS15190 0.83 -0.9 3,185,446 + -1.1 3,185,446 + +0.8 3,185,543 + -0.5 3,185,710 - BWI76_RS15195 0.19 -1.8 3,185,881 - BWI76_RS15195 0.65 -1.2 3,186,093 - +0.9 3,186,326 + BWI76_RS15200 0.34 +0.9 3,186,539 - BWI76_RS15200 0.57 +0.8 3,187,198 - +1.3 3,187,473 + BWI76_RS15205 0.24 -0.7 3,187,555 - BWI76_RS15205 0.30 +0.1
Or see this region's nucleotide sequence