Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14440 and BWI76_RS14445 are separated by 52 nucleotides BWI76_RS14445 and BWI76_RS14450 are separated by 132 nucleotides BWI76_RS14450 and BWI76_RS14455 overlap by 4 nucleotides
BWI76_RS14440: BWI76_RS14440 - glutamine ABC transporter permease GlnP, at 3,013,794 to 3,014,450
_RS14440
BWI76_RS14445: BWI76_RS14445 - amino acid ABC transporter substrate-binding protein, at 3,014,503 to 3,015,255
_RS14445
BWI76_RS14450: BWI76_RS14450 - hypothetical protein, at 3,015,388 to 3,015,939
_RS14450
BWI76_RS14455: BWI76_RS14455 - aspartate aminotransferase family protein, at 3,015,936 to 3,017,279
_RS14455
Position (kb)
3015
3016 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3014.457 kb on + strand at 3014.459 kb on + strand at 3014.465 kb on - strand at 3014.465 kb on - strand at 3014.467 kb on - strand at 3014.467 kb on - strand at 3014.467 kb on - strand at 3014.471 kb on + strand at 3014.479 kb on - strand at 3014.481 kb on + strand at 3014.484 kb on - strand at 3014.484 kb on - strand at 3014.508 kb on - strand at 3014.508 kb on - strand at 3014.556 kb on - strand at 3014.556 kb on - strand at 3014.556 kb on - strand at 3014.556 kb on - strand at 3014.556 kb on - strand at 3014.556 kb on - strand at 3014.559 kb on + strand at 3014.559 kb on + strand at 3014.577 kb on - strand at 3014.577 kb on - strand at 3014.579 kb on + strand, within BWI76_RS14445 at 3014.587 kb on - strand at 3014.600 kb on - strand, within BWI76_RS14445 at 3014.600 kb on - strand, within BWI76_RS14445 at 3014.614 kb on - strand at 3014.637 kb on + strand, within BWI76_RS14445 at 3014.637 kb on + strand, within BWI76_RS14445 at 3014.637 kb on + strand, within BWI76_RS14445 at 3014.645 kb on - strand, within BWI76_RS14445 at 3014.649 kb on + strand, within BWI76_RS14445 at 3014.657 kb on - strand, within BWI76_RS14445 at 3014.663 kb on + strand, within BWI76_RS14445 at 3014.698 kb on + strand, within BWI76_RS14445 at 3014.706 kb on + strand, within BWI76_RS14445 at 3014.706 kb on + strand, within BWI76_RS14445 at 3014.706 kb on + strand, within BWI76_RS14445 at 3014.706 kb on + strand, within BWI76_RS14445 at 3014.714 kb on - strand, within BWI76_RS14445 at 3014.714 kb on - strand, within BWI76_RS14445 at 3014.716 kb on + strand, within BWI76_RS14445 at 3014.716 kb on + strand, within BWI76_RS14445 at 3014.719 kb on + strand, within BWI76_RS14445 at 3014.727 kb on - strand, within BWI76_RS14445 at 3014.729 kb on + strand at 3014.729 kb on + strand, within BWI76_RS14445 at 3014.729 kb on + strand, within BWI76_RS14445 at 3014.730 kb on + strand, within BWI76_RS14445 at 3014.730 kb on + strand, within BWI76_RS14445 at 3014.730 kb on + strand, within BWI76_RS14445 at 3014.730 kb on + strand, within BWI76_RS14445 at 3014.737 kb on - strand, within BWI76_RS14445 at 3014.737 kb on - strand, within BWI76_RS14445 at 3014.738 kb on - strand, within BWI76_RS14445 at 3014.759 kb on - strand, within BWI76_RS14445 at 3014.759 kb on - strand, within BWI76_RS14445 at 3014.759 kb on - strand, within BWI76_RS14445 at 3014.759 kb on - strand, within BWI76_RS14445 at 3014.759 kb on - strand, within BWI76_RS14445 at 3014.759 kb on - strand, within BWI76_RS14445 at 3014.828 kb on + strand, within BWI76_RS14445 at 3014.828 kb on + strand, within BWI76_RS14445 at 3014.828 kb on + strand, within BWI76_RS14445 at 3014.828 kb on + strand, within BWI76_RS14445 at 3014.831 kb on + strand, within BWI76_RS14445 at 3014.831 kb on + strand, within BWI76_RS14445 at 3014.833 kb on + strand, within BWI76_RS14445 at 3014.833 kb on + strand, within BWI76_RS14445 at 3014.836 kb on - strand at 3014.836 kb on - strand, within BWI76_RS14445 at 3014.838 kb on + strand, within BWI76_RS14445 at 3014.905 kb on - strand, within BWI76_RS14445 at 3014.914 kb on - strand, within BWI76_RS14445 at 3014.924 kb on - strand, within BWI76_RS14445 at 3014.955 kb on + strand, within BWI76_RS14445 at 3014.968 kb on + strand, within BWI76_RS14445 at 3014.975 kb on - strand, within BWI76_RS14445 at 3015.148 kb on + strand, within BWI76_RS14445 at 3015.149 kb on - strand, within BWI76_RS14445 at 3015.149 kb on - strand, within BWI76_RS14445 at 3015.216 kb on - strand at 3015.283 kb on + strand at 3015.291 kb on - strand at 3015.407 kb on - strand at 3015.407 kb on - strand at 3015.420 kb on + strand at 3015.420 kb on - strand at 3015.426 kb on - strand at 3015.426 kb on - strand at 3015.426 kb on - strand at 3015.599 kb on + strand, within BWI76_RS14450 at 3015.600 kb on + strand, within BWI76_RS14450 at 3015.607 kb on - strand, within BWI76_RS14450 at 3015.614 kb on + strand, within BWI76_RS14450 at 3015.617 kb on - strand, within BWI76_RS14450 at 3015.810 kb on + strand, within BWI76_RS14450 at 3015.818 kb on - strand, within BWI76_RS14450 at 3015.979 kb on + strand at 3016.046 kb on + strand at 3016.054 kb on - strand at 3016.054 kb on - strand at 3016.054 kb on - strand at 3016.054 kb on - strand at 3016.054 kb on - strand at 3016.180 kb on - strand, within BWI76_RS14455 at 3016.242 kb on + strand, within BWI76_RS14455 at 3016.431 kb on + strand, within BWI76_RS14455 at 3016.439 kb on - strand, within BWI76_RS14455 at 3016.509 kb on - strand, within BWI76_RS14455 at 3016.509 kb on - strand, within BWI76_RS14455 at 3016.630 kb on - strand, within BWI76_RS14455
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,014,457 + -1.5 3,014,459 + -1.2 3,014,465 - -0.9 3,014,465 - -0.2 3,014,467 - +0.8 3,014,467 - +1.1 3,014,467 - -1.3 3,014,471 + +0.8 3,014,479 - -0.8 3,014,481 + -1.0 3,014,484 - -0.8 3,014,484 - -1.1 3,014,508 - +1.2 3,014,508 - +1.8 3,014,556 - +0.2 3,014,556 - -0.2 3,014,556 - -2.0 3,014,556 - +0.9 3,014,556 - -0.7 3,014,556 - -0.8 3,014,559 + +0.4 3,014,559 + -0.1 3,014,577 - +2.4 3,014,577 - -0.1 3,014,579 + BWI76_RS14445 0.10 +0.7 3,014,587 - -1.2 3,014,600 - BWI76_RS14445 0.13 -1.8 3,014,600 - BWI76_RS14445 0.13 +0.1 3,014,614 - +0.2 3,014,637 + BWI76_RS14445 0.18 +0.8 3,014,637 + BWI76_RS14445 0.18 +0.8 3,014,637 + BWI76_RS14445 0.18 -1.7 3,014,645 - BWI76_RS14445 0.19 -0.8 3,014,649 + BWI76_RS14445 0.19 -2.2 3,014,657 - BWI76_RS14445 0.20 -3.0 3,014,663 + BWI76_RS14445 0.21 +0.8 3,014,698 + BWI76_RS14445 0.26 +0.8 3,014,706 + BWI76_RS14445 0.27 -0.8 3,014,706 + BWI76_RS14445 0.27 +0.8 3,014,706 + BWI76_RS14445 0.27 -0.2 3,014,706 + BWI76_RS14445 0.27 +1.0 3,014,714 - BWI76_RS14445 0.28 +2.4 3,014,714 - BWI76_RS14445 0.28 -2.2 3,014,716 + BWI76_RS14445 0.28 -0.2 3,014,716 + BWI76_RS14445 0.28 -1.2 3,014,719 + BWI76_RS14445 0.29 -1.2 3,014,727 - BWI76_RS14445 0.30 -1.2 3,014,729 + +0.8 3,014,729 + BWI76_RS14445 0.30 +0.1 3,014,729 + BWI76_RS14445 0.30 -0.1 3,014,730 + BWI76_RS14445 0.30 +0.8 3,014,730 + BWI76_RS14445 0.30 +0.4 3,014,730 + BWI76_RS14445 0.30 +0.3 3,014,730 + BWI76_RS14445 0.30 -1.5 3,014,737 - BWI76_RS14445 0.31 -0.2 3,014,737 - BWI76_RS14445 0.31 -1.5 3,014,738 - BWI76_RS14445 0.31 -1.8 3,014,759 - BWI76_RS14445 0.34 -0.1 3,014,759 - BWI76_RS14445 0.34 -0.8 3,014,759 - BWI76_RS14445 0.34 -0.8 3,014,759 - BWI76_RS14445 0.34 -0.2 3,014,759 - BWI76_RS14445 0.34 +1.3 3,014,759 - BWI76_RS14445 0.34 -1.5 3,014,828 + BWI76_RS14445 0.43 -1.5 3,014,828 + BWI76_RS14445 0.43 +0.2 3,014,828 + BWI76_RS14445 0.43 +0.1 3,014,828 + BWI76_RS14445 0.43 -0.7 3,014,831 + BWI76_RS14445 0.44 -0.1 3,014,831 + BWI76_RS14445 0.44 +0.4 3,014,833 + BWI76_RS14445 0.44 +0.2 3,014,833 + BWI76_RS14445 0.44 +0.4 3,014,836 - -0.2 3,014,836 - BWI76_RS14445 0.44 +0.6 3,014,838 + BWI76_RS14445 0.44 -0.4 3,014,905 - BWI76_RS14445 0.53 -0.2 3,014,914 - BWI76_RS14445 0.55 -1.5 3,014,924 - BWI76_RS14445 0.56 -0.3 3,014,955 + BWI76_RS14445 0.60 -1.2 3,014,968 + BWI76_RS14445 0.62 -0.2 3,014,975 - BWI76_RS14445 0.63 -0.2 3,015,148 + BWI76_RS14445 0.86 -0.3 3,015,149 - BWI76_RS14445 0.86 -0.8 3,015,149 - BWI76_RS14445 0.86 +0.8 3,015,216 - +0.1 3,015,283 + +2.4 3,015,291 - +0.9 3,015,407 - -0.8 3,015,407 - +0.1 3,015,420 + -1.2 3,015,420 - -1.1 3,015,426 - -0.6 3,015,426 - +0.5 3,015,426 - -1.8 3,015,599 + BWI76_RS14450 0.38 -2.5 3,015,600 + BWI76_RS14450 0.38 -0.7 3,015,607 - BWI76_RS14450 0.40 +0.8 3,015,614 + BWI76_RS14450 0.41 -0.2 3,015,617 - BWI76_RS14450 0.41 -1.6 3,015,810 + BWI76_RS14450 0.76 -0.3 3,015,818 - BWI76_RS14450 0.78 -0.3 3,015,979 + +0.7 3,016,046 + +0.2 3,016,054 - -0.4 3,016,054 - -0.2 3,016,054 - +0.2 3,016,054 - +1.1 3,016,054 - -0.4 3,016,180 - BWI76_RS14455 0.18 +0.9 3,016,242 + BWI76_RS14455 0.23 -0.7 3,016,431 + BWI76_RS14455 0.37 +0.2 3,016,439 - BWI76_RS14455 0.37 +0.4 3,016,509 - BWI76_RS14455 0.43 -0.3 3,016,509 - BWI76_RS14455 0.43 -0.0 3,016,630 - BWI76_RS14455 0.52 +0.5
Or see this region's nucleotide sequence