Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13165 and BWI76_RS13170 are separated by 48 nucleotides BWI76_RS13170 and BWI76_RS13175 are separated by 15 nucleotides BWI76_RS13175 and BWI76_RS13180 overlap by 4 nucleotides
BWI76_RS13165: BWI76_RS13165 - ATP-dependent RNA helicase HrpA, at 2,744,380 to 2,748,282
_RS13165
BWI76_RS13170: BWI76_RS13170 - methyltransferase, at 2,748,331 to 2,748,990
_RS13170
BWI76_RS13175: BWI76_RS13175 - NodT family RND efflux system outer membrane lipoprotein, at 2,749,006 to 2,750,385
_RS13175
BWI76_RS13180: BWI76_RS13180 - multidrug transporter EmrA, at 2,750,382 to 2,751,467
_RS13180
Position (kb)
2749
2750
2751 Strain fitness (log2 ratio)
-2
-1
0
1 at 2748.216 kb on + strand at 2748.513 kb on + strand, within BWI76_RS13170 at 2748.726 kb on + strand, within BWI76_RS13170 at 2748.768 kb on - strand, within BWI76_RS13170 at 2749.948 kb on + strand, within BWI76_RS13175 at 2750.018 kb on + strand, within BWI76_RS13175 at 2750.018 kb on + strand, within BWI76_RS13175 at 2750.911 kb on + strand, within BWI76_RS13180 at 2750.945 kb on - strand, within BWI76_RS13180 at 2751.173 kb on + strand, within BWI76_RS13180 at 2751.181 kb on - strand, within BWI76_RS13180
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,748,216 + +0.7 2,748,513 + BWI76_RS13170 0.28 -0.1 2,748,726 + BWI76_RS13170 0.60 +0.8 2,748,768 - BWI76_RS13170 0.66 +0.2 2,749,948 + BWI76_RS13175 0.68 +0.9 2,750,018 + BWI76_RS13175 0.73 +0.9 2,750,018 + BWI76_RS13175 0.73 +0.6 2,750,911 + BWI76_RS13180 0.49 +0.4 2,750,945 - BWI76_RS13180 0.52 +0.8 2,751,173 + BWI76_RS13180 0.73 -2.5 2,751,181 - BWI76_RS13180 0.74 -1.0
Or see this region's nucleotide sequence