Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12575 and BWI76_RS12580 overlap by 4 nucleotides BWI76_RS12580 and BWI76_RS12585 overlap by 4 nucleotides BWI76_RS12585 and BWI76_RS12590 are separated by 264 nucleotides
BWI76_RS12575: BWI76_RS12575 - FAD-dependent oxidoreductase, at 2,625,455 to 2,626,714
_RS12575
BWI76_RS12580: BWI76_RS12580 - oxidoreductase, at 2,626,711 to 2,627,430
_RS12580
BWI76_RS12585: BWI76_RS12585 - transcriptional regulator, at 2,627,427 to 2,627,861
_RS12585
BWI76_RS12590: BWI76_RS12590 - helix-turn-helix-type transcriptional regulator, at 2,628,126 to 2,628,854
_RS12590
Position (kb)
2627
2628 Strain fitness (log2 ratio)
-1
0
1 at 2626.640 kb on + strand at 2626.709 kb on + strand at 2626.785 kb on + strand, within BWI76_RS12580 at 2626.786 kb on + strand, within BWI76_RS12580 at 2626.998 kb on - strand, within BWI76_RS12580 at 2627.336 kb on - strand, within BWI76_RS12580 at 2627.404 kb on + strand at 2627.610 kb on + strand, within BWI76_RS12585 at 2627.726 kb on + strand, within BWI76_RS12585 at 2627.801 kb on + strand, within BWI76_RS12585 at 2627.815 kb on + strand, within BWI76_RS12585 at 2627.868 kb on + strand at 2627.868 kb on + strand at 2627.868 kb on + strand at 2628.058 kb on - strand at 2628.400 kb on - strand, within BWI76_RS12590 at 2628.416 kb on - strand, within BWI76_RS12590 at 2628.484 kb on - strand, within BWI76_RS12590 at 2628.771 kb on - strand, within BWI76_RS12590 at 2628.771 kb on - strand, within BWI76_RS12590
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,626,640 + +0.1 2,626,709 + +0.6 2,626,785 + BWI76_RS12580 0.10 +0.5 2,626,786 + BWI76_RS12580 0.10 +0.2 2,626,998 - BWI76_RS12580 0.40 +0.2 2,627,336 - BWI76_RS12580 0.87 +0.3 2,627,404 + +0.3 2,627,610 + BWI76_RS12585 0.42 +0.6 2,627,726 + BWI76_RS12585 0.69 +0.6 2,627,801 + BWI76_RS12585 0.86 +0.8 2,627,815 + BWI76_RS12585 0.89 -0.1 2,627,868 + +0.1 2,627,868 + -0.1 2,627,868 + +0.4 2,628,058 - -0.2 2,628,400 - BWI76_RS12590 0.38 +0.3 2,628,416 - BWI76_RS12590 0.40 +0.6 2,628,484 - BWI76_RS12590 0.49 -0.5 2,628,771 - BWI76_RS12590 0.88 +0.3 2,628,771 - BWI76_RS12590 0.88 +0.8
Or see this region's nucleotide sequence