Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12545 and BWI76_RS12550 are separated by 14 nucleotides BWI76_RS12550 and BWI76_RS12555 overlap by 4 nucleotides BWI76_RS12555 and BWI76_RS12560 overlap by 4 nucleotides
BWI76_RS12545: BWI76_RS12545 - hypothetical protein, at 2,621,007 to 2,621,966
_RS12545
BWI76_RS12550: BWI76_RS12550 - hypothetical protein, at 2,621,981 to 2,622,514
_RS12550
BWI76_RS12555: BWI76_RS12555 - hypothetical protein, at 2,622,511 to 2,623,035
_RS12555
BWI76_RS12560: BWI76_RS12560 - hypothetical protein, at 2,623,032 to 2,623,517
_RS12560
BWI76_RS12565: BWI76_RS12565 - SAM-dependent methyltransferase, at 2,623,514 to 2,624,734
_RS12565
Position (kb)
2621
2622
2623 Strain fitness (log2 ratio)
-1
0
1
2 at 2621.035 kb on - strand at 2621.048 kb on + strand at 2621.120 kb on + strand, within BWI76_RS12545 at 2621.475 kb on - strand, within BWI76_RS12545 at 2621.475 kb on - strand, within BWI76_RS12545 at 2622.111 kb on + strand, within BWI76_RS12550 at 2622.264 kb on - strand, within BWI76_RS12550 at 2622.699 kb on - strand, within BWI76_RS12555 at 2622.699 kb on - strand, within BWI76_RS12555 at 2622.806 kb on + strand, within BWI76_RS12555 at 2622.814 kb on - strand, within BWI76_RS12555 at 2622.838 kb on - strand, within BWI76_RS12555 at 2623.224 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,621,035 - -0.1 2,621,048 + +0.5 2,621,120 + BWI76_RS12545 0.12 +1.0 2,621,475 - BWI76_RS12545 0.49 +0.0 2,621,475 - BWI76_RS12545 0.49 -0.1 2,622,111 + BWI76_RS12550 0.24 +0.7 2,622,264 - BWI76_RS12550 0.53 +2.1 2,622,699 - BWI76_RS12555 0.36 -0.3 2,622,699 - BWI76_RS12555 0.36 -0.1 2,622,806 + BWI76_RS12555 0.56 +0.6 2,622,814 - BWI76_RS12555 0.58 +0.3 2,622,838 - BWI76_RS12555 0.62 +0.9 2,623,224 + -0.1
Or see this region's nucleotide sequence