Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12180 and BWI76_RS12185 overlap by 11 nucleotides BWI76_RS12185 and BWI76_RS12190 are separated by 7 nucleotides BWI76_RS12190 and BWI76_RS12195 are separated by 2 nucleotides BWI76_RS12195 and BWI76_RS12200 overlap by 50 nucleotides BWI76_RS12200 and BWI76_RS12205 overlap by 4 nucleotides
BWI76_RS12180: BWI76_RS12180 - membrane protein, at 2,552,135 to 2,552,524
_RS12180
BWI76_RS12185: BWI76_RS12185 - hypothetical protein, at 2,552,514 to 2,552,792
_RS12185
BWI76_RS12190: BWI76_RS12190 - glycoside hydrolase family protein, at 2,552,800 to 2,553,429
_RS12190
BWI76_RS12195: BWI76_RS12195 - hypothetical protein, at 2,553,432 to 2,553,707
_RS12195
BWI76_RS12200: BWI76_RS12200 - hypothetical protein, at 2,553,658 to 2,553,852
_RS12200
BWI76_RS12205: BWI76_RS12205 - hypothetical protein, at 2,553,849 to 2,554,133
_RS12205
Position (kb)
2553
2554 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2552.502 kb on - strand at 2552.596 kb on + strand, within BWI76_RS12185 at 2552.606 kb on - strand, within BWI76_RS12185 at 2552.653 kb on - strand, within BWI76_RS12185 at 2553.015 kb on + strand at 2553.033 kb on + strand, within BWI76_RS12190 at 2553.033 kb on + strand, within BWI76_RS12190 at 2553.036 kb on - strand, within BWI76_RS12190 at 2553.036 kb on - strand, within BWI76_RS12190 at 2553.045 kb on + strand at 2553.047 kb on + strand, within BWI76_RS12190 at 2553.054 kb on + strand at 2553.081 kb on - strand, within BWI76_RS12190 at 2553.140 kb on - strand, within BWI76_RS12190 at 2553.140 kb on - strand, within BWI76_RS12190 at 2553.160 kb on - strand, within BWI76_RS12190 at 2553.160 kb on - strand, within BWI76_RS12190 at 2553.287 kb on - strand, within BWI76_RS12190 at 2553.287 kb on - strand, within BWI76_RS12190 at 2553.289 kb on + strand, within BWI76_RS12190 at 2553.400 kb on - strand at 2553.496 kb on + strand, within BWI76_RS12195 at 2553.504 kb on - strand, within BWI76_RS12195 at 2553.553 kb on - strand, within BWI76_RS12195 at 2553.578 kb on - strand, within BWI76_RS12195 at 2553.615 kb on - strand at 2553.627 kb on - strand, within BWI76_RS12195 at 2553.719 kb on + strand, within BWI76_RS12200 at 2553.751 kb on - strand, within BWI76_RS12200 at 2553.856 kb on + strand at 2553.879 kb on + strand, within BWI76_RS12205 at 2553.968 kb on + strand, within BWI76_RS12205 at 2554.104 kb on + strand, within BWI76_RS12205 at 2554.127 kb on + strand at 2554.152 kb on + strand at 2554.160 kb on - strand at 2554.160 kb on - strand at 2554.160 kb on - strand at 2554.237 kb on + strand at 2554.245 kb on - strand at 2554.255 kb on - strand at 2554.279 kb on + strand at 2554.287 kb on - strand at 2554.287 kb on - strand at 2554.293 kb on - strand at 2554.294 kb on + strand at 2554.303 kb on + strand at 2554.308 kb on + strand at 2554.391 kb on + strand at 2554.399 kb on - strand at 2554.406 kb on - strand at 2554.406 kb on - strand at 2554.469 kb on - strand at 2554.505 kb on - strand at 2554.511 kb on - strand at 2554.513 kb on - strand at 2554.513 kb on - strand at 2554.516 kb on + strand at 2554.516 kb on + strand at 2554.516 kb on + strand at 2554.516 kb on + strand at 2554.516 kb on + strand at 2554.533 kb on - strand at 2554.543 kb on - strand at 2554.565 kb on + strand at 2554.571 kb on + strand at 2554.580 kb on + strand at 2554.580 kb on + strand at 2554.588 kb on - strand at 2554.689 kb on + strand at 2554.690 kb on + strand at 2554.694 kb on + strand at 2554.694 kb on + strand at 2554.699 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,552,502 - +0.9 2,552,596 + BWI76_RS12185 0.29 -0.1 2,552,606 - BWI76_RS12185 0.33 -0.6 2,552,653 - BWI76_RS12185 0.50 +0.9 2,553,015 + -3.5 2,553,033 + BWI76_RS12190 0.37 -0.2 2,553,033 + BWI76_RS12190 0.37 -0.4 2,553,036 - BWI76_RS12190 0.37 +0.9 2,553,036 - BWI76_RS12190 0.37 -2.1 2,553,045 + -0.1 2,553,047 + BWI76_RS12190 0.39 +0.2 2,553,054 + +0.6 2,553,081 - BWI76_RS12190 0.45 -2.0 2,553,140 - BWI76_RS12190 0.54 -1.8 2,553,140 - BWI76_RS12190 0.54 -0.2 2,553,160 - BWI76_RS12190 0.57 -0.3 2,553,160 - BWI76_RS12190 0.57 -0.8 2,553,287 - BWI76_RS12190 0.77 +0.2 2,553,287 - BWI76_RS12190 0.77 -1.9 2,553,289 + BWI76_RS12190 0.78 -0.1 2,553,400 - -0.5 2,553,496 + BWI76_RS12195 0.23 -0.0 2,553,504 - BWI76_RS12195 0.26 -0.6 2,553,553 - BWI76_RS12195 0.44 -2.2 2,553,578 - BWI76_RS12195 0.53 -0.1 2,553,615 - +0.9 2,553,627 - BWI76_RS12195 0.71 -1.2 2,553,719 + BWI76_RS12200 0.31 -1.1 2,553,751 - BWI76_RS12200 0.48 -0.0 2,553,856 + -1.8 2,553,879 + BWI76_RS12205 0.11 -0.4 2,553,968 + BWI76_RS12205 0.42 -0.9 2,554,104 + BWI76_RS12205 0.89 -1.3 2,554,127 + +0.4 2,554,152 + +0.1 2,554,160 - -1.4 2,554,160 - +0.5 2,554,160 - -0.4 2,554,237 + -0.9 2,554,245 - -1.8 2,554,255 - -0.0 2,554,279 + +0.2 2,554,287 - -0.5 2,554,287 - -3.0 2,554,293 - -3.4 2,554,294 + -0.8 2,554,303 + -0.4 2,554,308 + -0.5 2,554,391 + -0.5 2,554,399 - +1.7 2,554,406 - -1.0 2,554,406 - -0.7 2,554,469 - +1.1 2,554,505 - +0.6 2,554,511 - -0.9 2,554,513 - -1.1 2,554,513 - -2.6 2,554,516 + -0.2 2,554,516 + -0.6 2,554,516 + -0.0 2,554,516 + -0.6 2,554,516 + -0.2 2,554,533 - -1.1 2,554,543 - -0.0 2,554,565 + -1.0 2,554,571 + -0.4 2,554,580 + -0.2 2,554,580 + +0.5 2,554,588 - +1.0 2,554,689 + +0.1 2,554,690 + -0.6 2,554,694 + -0.4 2,554,694 + +0.2 2,554,699 + -1.6
Or see this region's nucleotide sequence