Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11830 and BWI76_RS11835 are separated by 77 nucleotides BWI76_RS11835 and BWI76_RS11840 are separated by 32 nucleotides BWI76_RS11840 and BWI76_RS11845 overlap by 1 nucleotides
BWI76_RS11830: BWI76_RS11830 - hypothetical protein, at 2,487,447 to 2,488,394
_RS11830
BWI76_RS11835: BWI76_RS11835 - putative regulatory protein MarR, at 2,488,472 to 2,488,927
_RS11835
BWI76_RS11840: BWI76_RS11840 - tryptophan synthase subunit alpha, at 2,488,960 to 2,489,769
_RS11840
BWI76_RS11845: BWI76_RS11845 - tryptophan synthase subunit beta, at 2,489,769 to 2,490,962
_RS11845
Position (kb)
2488
2489
2490 Strain fitness (log2 ratio)
-2
-1
0
1 at 2487.964 kb on + strand, within BWI76_RS11830 at 2488.110 kb on + strand, within BWI76_RS11830 at 2488.133 kb on + strand, within BWI76_RS11830 at 2488.242 kb on + strand, within BWI76_RS11830 at 2488.718 kb on + strand, within BWI76_RS11835 at 2488.873 kb on - strand, within BWI76_RS11835 at 2489.110 kb on + strand, within BWI76_RS11840 at 2489.118 kb on - strand, within BWI76_RS11840 at 2489.335 kb on - strand, within BWI76_RS11840 at 2489.732 kb on + strand at 2489.811 kb on + strand at 2490.020 kb on - strand, within BWI76_RS11845 at 2490.126 kb on + strand, within BWI76_RS11845
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,487,964 + BWI76_RS11830 0.55 +1.0 2,488,110 + BWI76_RS11830 0.70 -0.3 2,488,133 + BWI76_RS11830 0.72 +0.6 2,488,242 + BWI76_RS11830 0.84 -0.1 2,488,718 + BWI76_RS11835 0.54 +0.2 2,488,873 - BWI76_RS11835 0.88 +0.2 2,489,110 + BWI76_RS11840 0.19 -2.1 2,489,118 - BWI76_RS11840 0.20 -2.0 2,489,335 - BWI76_RS11840 0.46 -2.3 2,489,732 + -1.6 2,489,811 + -2.5 2,490,020 - BWI76_RS11845 0.21 +0.9 2,490,126 + BWI76_RS11845 0.30 -1.0
Or see this region's nucleotide sequence