Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10975 and BWI76_RS10980 are separated by 124 nucleotides BWI76_RS10980 and BWI76_RS10985 are separated by 285 nucleotides BWI76_RS10985 and BWI76_RS10990 are separated by 72 nucleotides BWI76_RS10990 and BWI76_RS10995 are separated by 122 nucleotides BWI76_RS10995 and BWI76_RS11000 are separated by 135 nucleotides
BWI76_RS10975: BWI76_RS10975 - N-methyltryptophan oxidase, at 2,316,229 to 2,317,347
_RS10975
BWI76_RS10980: BWI76_RS10980 - biofilm formation regulatory protein BssS, at 2,317,472 to 2,317,726
_RS10980
BWI76_RS10985: BWI76_RS10985 - DNA damage-inducible protein I, at 2,318,012 to 2,318,257
_RS10985
BWI76_RS10990: BWI76_RS10990 - dihydroorotase, at 2,318,330 to 2,319,376
_RS10990
BWI76_RS10995: BWI76_RS10995 - lipoprotein, at 2,319,499 to 2,320,059
_RS10995
BWI76_RS11000: BWI76_RS11000 - glutaredoxin 2, at 2,320,195 to 2,320,842
_RS11000
Position (kb)
2318
2319
2320 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2317.337 kb on - strand at 2317.344 kb on + strand at 2317.352 kb on + strand at 2317.361 kb on - strand at 2317.367 kb on - strand at 2317.433 kb on - strand at 2317.492 kb on - strand at 2317.497 kb on - strand at 2317.517 kb on - strand, within BWI76_RS10980 at 2317.518 kb on - strand, within BWI76_RS10980 at 2317.638 kb on + strand, within BWI76_RS10980 at 2317.643 kb on + strand, within BWI76_RS10980 at 2317.670 kb on + strand, within BWI76_RS10980 at 2317.676 kb on - strand, within BWI76_RS10980 at 2317.680 kb on + strand, within BWI76_RS10980 at 2317.690 kb on + strand, within BWI76_RS10980 at 2317.697 kb on + strand, within BWI76_RS10980 at 2317.697 kb on + strand, within BWI76_RS10980 at 2317.698 kb on - strand, within BWI76_RS10980 at 2317.705 kb on - strand at 2317.705 kb on - strand at 2317.726 kb on + strand at 2317.927 kb on + strand at 2317.935 kb on - strand at 2317.945 kb on - strand at 2318.025 kb on + strand at 2318.033 kb on - strand at 2318.033 kb on - strand at 2318.035 kb on - strand at 2318.045 kb on - strand, within BWI76_RS10985 at 2318.134 kb on + strand, within BWI76_RS10985 at 2318.152 kb on - strand, within BWI76_RS10985 at 2318.193 kb on - strand, within BWI76_RS10985 at 2318.193 kb on - strand, within BWI76_RS10985 at 2318.439 kb on + strand, within BWI76_RS10990 at 2318.567 kb on + strand at 2318.866 kb on - strand, within BWI76_RS10990 at 2319.099 kb on - strand, within BWI76_RS10990 at 2319.372 kb on + strand at 2319.431 kb on + strand at 2319.761 kb on + strand, within BWI76_RS10995 at 2319.769 kb on - strand, within BWI76_RS10995 at 2319.838 kb on - strand, within BWI76_RS10995 at 2319.947 kb on - strand, within BWI76_RS10995 at 2319.989 kb on + strand, within BWI76_RS10995 at 2320.007 kb on + strand at 2320.007 kb on - strand at 2320.020 kb on - strand at 2320.176 kb on + strand at 2320.363 kb on - strand at 2320.363 kb on - strand, within BWI76_RS11000
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,317,337 - -0.3 2,317,344 + -0.6 2,317,352 + +0.6 2,317,361 - +2.3 2,317,367 - +1.2 2,317,433 - +0.2 2,317,492 - +0.5 2,317,497 - +0.2 2,317,517 - BWI76_RS10980 0.18 -0.0 2,317,518 - BWI76_RS10980 0.18 +0.3 2,317,638 + BWI76_RS10980 0.65 +0.7 2,317,643 + BWI76_RS10980 0.67 -0.3 2,317,670 + BWI76_RS10980 0.78 -0.6 2,317,676 - BWI76_RS10980 0.80 +1.3 2,317,680 + BWI76_RS10980 0.82 +0.1 2,317,690 + BWI76_RS10980 0.85 +0.2 2,317,697 + BWI76_RS10980 0.88 -2.4 2,317,697 + BWI76_RS10980 0.88 +0.6 2,317,698 - BWI76_RS10980 0.89 +0.1 2,317,705 - -0.2 2,317,705 - -0.5 2,317,726 + +0.8 2,317,927 + -2.1 2,317,935 - -0.4 2,317,945 - +0.2 2,318,025 + -1.5 2,318,033 - -0.8 2,318,033 - -0.3 2,318,035 - +0.5 2,318,045 - BWI76_RS10985 0.13 -0.4 2,318,134 + BWI76_RS10985 0.50 -0.7 2,318,152 - BWI76_RS10985 0.57 +0.1 2,318,193 - BWI76_RS10985 0.74 +0.7 2,318,193 - BWI76_RS10985 0.74 -0.0 2,318,439 + BWI76_RS10990 0.10 -1.9 2,318,567 + +1.7 2,318,866 - BWI76_RS10990 0.51 -3.0 2,319,099 - BWI76_RS10990 0.73 +1.7 2,319,372 + -4.3 2,319,431 + -0.3 2,319,761 + BWI76_RS10995 0.47 +0.4 2,319,769 - BWI76_RS10995 0.48 +2.9 2,319,838 - BWI76_RS10995 0.60 +0.0 2,319,947 - BWI76_RS10995 0.80 +0.2 2,319,989 + BWI76_RS10995 0.87 -0.0 2,320,007 + -1.9 2,320,007 - +1.5 2,320,020 - -0.2 2,320,176 + -0.1 2,320,363 - -0.3 2,320,363 - BWI76_RS11000 0.26 +0.7
Or see this region's nucleotide sequence