Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10460 and BWI76_RS10465 are separated by 271 nucleotides BWI76_RS10465 and BWI76_RS10470 are separated by 261 nucleotides
BWI76_RS10460: BWI76_RS10460 - transcriptional regulator LeuO, at 2,209,750 to 2,210,697
_RS10460
BWI76_RS10465: BWI76_RS10465 - hypothetical protein, at 2,210,969 to 2,211,493
_RS10465
BWI76_RS10470: BWI76_RS10470 - iron ABC transporter substrate-binding protein, at 2,211,755 to 2,212,771
_RS10470
Position (kb)
2210
2211
2212 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2209.997 kb on + strand, within BWI76_RS10460 at 2210.088 kb on + strand, within BWI76_RS10460 at 2210.096 kb on - strand, within BWI76_RS10460 at 2210.171 kb on + strand, within BWI76_RS10460 at 2210.179 kb on - strand, within BWI76_RS10460 at 2210.226 kb on - strand, within BWI76_RS10460 at 2210.410 kb on + strand, within BWI76_RS10460 at 2210.425 kb on + strand, within BWI76_RS10460 at 2210.425 kb on + strand, within BWI76_RS10460 at 2210.435 kb on + strand, within BWI76_RS10460 at 2210.435 kb on + strand, within BWI76_RS10460 at 2210.443 kb on - strand, within BWI76_RS10460 at 2210.550 kb on - strand, within BWI76_RS10460 at 2210.567 kb on + strand, within BWI76_RS10460 at 2210.580 kb on + strand, within BWI76_RS10460 at 2210.612 kb on + strand at 2210.633 kb on - strand at 2210.638 kb on + strand at 2210.638 kb on + strand at 2210.646 kb on - strand at 2210.646 kb on - strand at 2210.646 kb on - strand at 2210.646 kb on - strand at 2210.655 kb on + strand at 2210.655 kb on + strand at 2210.663 kb on + strand at 2210.691 kb on + strand at 2210.696 kb on + strand at 2210.754 kb on - strand at 2210.910 kb on - strand at 2210.924 kb on - strand at 2210.939 kb on + strand at 2210.940 kb on + strand at 2210.945 kb on + strand at 2210.953 kb on - strand at 2210.958 kb on - strand at 2210.963 kb on + strand at 2210.963 kb on + strand at 2210.966 kb on - strand at 2210.971 kb on - strand at 2210.971 kb on - strand at 2210.971 kb on - strand at 2210.988 kb on + strand at 2210.988 kb on + strand at 2211.011 kb on + strand at 2211.119 kb on + strand, within BWI76_RS10465 at 2211.192 kb on - strand, within BWI76_RS10465 at 2211.288 kb on + strand, within BWI76_RS10465 at 2211.322 kb on + strand, within BWI76_RS10465 at 2211.330 kb on - strand, within BWI76_RS10465 at 2211.383 kb on - strand, within BWI76_RS10465 at 2211.467 kb on - strand at 2211.488 kb on + strand at 2211.491 kb on - strand at 2211.500 kb on + strand at 2211.501 kb on + strand at 2211.501 kb on + strand at 2211.501 kb on + strand at 2211.508 kb on - strand at 2211.508 kb on - strand at 2211.508 kb on - strand at 2211.509 kb on - strand at 2211.509 kb on - strand at 2211.509 kb on - strand at 2211.601 kb on + strand at 2211.612 kb on + strand at 2211.612 kb on + strand at 2211.612 kb on + strand at 2211.616 kb on + strand at 2211.620 kb on - strand at 2211.682 kb on - strand at 2211.693 kb on + strand at 2211.701 kb on - strand at 2211.718 kb on + strand at 2211.718 kb on + strand at 2211.718 kb on + strand at 2211.718 kb on + strand at 2211.724 kb on + strand at 2211.726 kb on - strand at 2211.726 kb on - strand at 2211.728 kb on - strand at 2211.738 kb on + strand at 2211.738 kb on + strand at 2211.738 kb on + strand at 2211.745 kb on + strand at 2211.746 kb on - strand at 2211.753 kb on - strand at 2211.929 kb on - strand, within BWI76_RS10470 at 2211.937 kb on + strand, within BWI76_RS10470 at 2211.945 kb on - strand, within BWI76_RS10470 at 2212.080 kb on + strand, within BWI76_RS10470
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,209,997 + BWI76_RS10460 0.26 +2.4 2,210,088 + BWI76_RS10460 0.36 +0.1 2,210,096 - BWI76_RS10460 0.36 -0.9 2,210,171 + BWI76_RS10460 0.44 -2.8 2,210,179 - BWI76_RS10460 0.45 -0.9 2,210,226 - BWI76_RS10460 0.50 +1.8 2,210,410 + BWI76_RS10460 0.70 +0.6 2,210,425 + BWI76_RS10460 0.71 -0.2 2,210,425 + BWI76_RS10460 0.71 +0.1 2,210,435 + BWI76_RS10460 0.72 +0.3 2,210,435 + BWI76_RS10460 0.72 +0.7 2,210,443 - BWI76_RS10460 0.73 -0.0 2,210,550 - BWI76_RS10460 0.84 +0.8 2,210,567 + BWI76_RS10460 0.86 -0.7 2,210,580 + BWI76_RS10460 0.88 +0.4 2,210,612 + -0.1 2,210,633 - +0.5 2,210,638 + -0.0 2,210,638 + -2.3 2,210,646 - -0.2 2,210,646 - +0.0 2,210,646 - -0.9 2,210,646 - -0.8 2,210,655 + +0.3 2,210,655 + -0.2 2,210,663 + +1.2 2,210,691 + +0.5 2,210,696 + +0.8 2,210,754 - +0.1 2,210,910 - +0.1 2,210,924 - +2.4 2,210,939 + +0.4 2,210,940 + +0.6 2,210,945 + -0.0 2,210,953 - +4.1 2,210,958 - -2.3 2,210,963 + +0.6 2,210,963 + -0.8 2,210,966 - +1.4 2,210,971 - -3.3 2,210,971 - -1.2 2,210,971 - -0.1 2,210,988 + -0.8 2,210,988 + -1.8 2,211,011 + -0.2 2,211,119 + BWI76_RS10465 0.29 -0.8 2,211,192 - BWI76_RS10465 0.42 -0.2 2,211,288 + BWI76_RS10465 0.61 -1.3 2,211,322 + BWI76_RS10465 0.67 -0.8 2,211,330 - BWI76_RS10465 0.69 -1.8 2,211,383 - BWI76_RS10465 0.79 -0.5 2,211,467 - -1.6 2,211,488 + -0.9 2,211,491 - -2.0 2,211,500 + +0.8 2,211,501 + -0.2 2,211,501 + +0.8 2,211,501 + +1.0 2,211,508 - +0.2 2,211,508 - -1.4 2,211,508 - -0.2 2,211,509 - -0.8 2,211,509 - -1.3 2,211,509 - -0.1 2,211,601 + +0.6 2,211,612 + -0.0 2,211,612 + -0.5 2,211,612 + -0.8 2,211,616 + +0.1 2,211,620 - +0.5 2,211,682 - -1.0 2,211,693 + +0.8 2,211,701 - -1.5 2,211,718 + -1.8 2,211,718 + +1.2 2,211,718 + +0.6 2,211,718 + +0.6 2,211,724 + -0.2 2,211,726 - -1.5 2,211,726 - -2.0 2,211,728 - -0.5 2,211,738 + +0.0 2,211,738 + +1.0 2,211,738 + -0.9 2,211,745 + -0.2 2,211,746 - -0.8 2,211,753 - +0.1 2,211,929 - BWI76_RS10470 0.17 +0.2 2,211,937 + BWI76_RS10470 0.18 -0.2 2,211,945 - BWI76_RS10470 0.19 +0.5 2,212,080 + BWI76_RS10470 0.32 +0.4
Or see this region's nucleotide sequence