Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10315 and BWI76_RS10320 are separated by 79 nucleotides BWI76_RS10320 and BWI76_RS10325 are separated by 199 nucleotides
BWI76_RS10315: BWI76_RS10315 - MFS transporter, at 2,183,574 to 2,184,866
_RS10315
BWI76_RS10320: BWI76_RS10320 - alpha-glucosidase/alpha-galactosidase, at 2,184,946 to 2,186,253
_RS10320
BWI76_RS10325: BWI76_RS10325 - AraC family transcriptional regulator, at 2,186,453 to 2,187,292
_RS10325
Position (kb)
2184
2185
2186
2187 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2184.087 kb on + strand, within BWI76_RS10315 at 2184.097 kb on + strand, within BWI76_RS10315 at 2184.325 kb on + strand, within BWI76_RS10315 at 2184.325 kb on + strand, within BWI76_RS10315 at 2184.332 kb on - strand, within BWI76_RS10315 at 2184.535 kb on - strand, within BWI76_RS10315 at 2184.717 kb on + strand, within BWI76_RS10315 at 2184.752 kb on + strand at 2184.760 kb on - strand at 2184.934 kb on + strand at 2185.055 kb on + strand at 2185.058 kb on - strand at 2185.224 kb on - strand, within BWI76_RS10320 at 2185.402 kb on - strand at 2185.475 kb on + strand, within BWI76_RS10320 at 2185.547 kb on + strand, within BWI76_RS10320 at 2185.569 kb on + strand, within BWI76_RS10320 at 2185.710 kb on + strand, within BWI76_RS10320 at 2185.730 kb on - strand, within BWI76_RS10320 at 2185.796 kb on + strand, within BWI76_RS10320 at 2185.868 kb on - strand, within BWI76_RS10320 at 2186.156 kb on + strand at 2186.159 kb on - strand at 2186.183 kb on + strand at 2186.403 kb on - strand at 2186.469 kb on + strand at 2186.647 kb on - strand, within BWI76_RS10325 at 2186.723 kb on - strand, within BWI76_RS10325 at 2186.939 kb on - strand, within BWI76_RS10325 at 2186.960 kb on - strand, within BWI76_RS10325 at 2187.013 kb on - strand, within BWI76_RS10325 at 2187.013 kb on - strand, within BWI76_RS10325 at 2187.042 kb on - strand, within BWI76_RS10325 at 2187.067 kb on + strand, within BWI76_RS10325 at 2187.067 kb on + strand, within BWI76_RS10325 at 2187.075 kb on + strand, within BWI76_RS10325 at 2187.090 kb on - strand, within BWI76_RS10325 at 2187.131 kb on - strand, within BWI76_RS10325 at 2187.187 kb on - strand, within BWI76_RS10325 at 2187.187 kb on - strand, within BWI76_RS10325 at 2187.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,184,087 + BWI76_RS10315 0.40 -0.6 2,184,097 + BWI76_RS10315 0.40 +1.3 2,184,325 + BWI76_RS10315 0.58 +0.3 2,184,325 + BWI76_RS10315 0.58 +2.2 2,184,332 - BWI76_RS10315 0.59 -0.7 2,184,535 - BWI76_RS10315 0.74 +0.2 2,184,717 + BWI76_RS10315 0.88 -0.2 2,184,752 + -0.5 2,184,760 - -0.2 2,184,934 + -0.0 2,185,055 + -0.0 2,185,058 - +1.4 2,185,224 - BWI76_RS10320 0.21 +0.4 2,185,402 - -0.8 2,185,475 + BWI76_RS10320 0.40 +0.9 2,185,547 + BWI76_RS10320 0.46 +1.4 2,185,569 + BWI76_RS10320 0.48 +0.4 2,185,710 + BWI76_RS10320 0.58 +0.4 2,185,730 - BWI76_RS10320 0.60 +0.6 2,185,796 + BWI76_RS10320 0.65 -1.2 2,185,868 - BWI76_RS10320 0.70 +1.1 2,186,156 + -0.7 2,186,159 - +0.8 2,186,183 + +0.2 2,186,403 - +0.2 2,186,469 + -0.5 2,186,647 - BWI76_RS10325 0.23 +0.4 2,186,723 - BWI76_RS10325 0.32 +0.6 2,186,939 - BWI76_RS10325 0.58 +2.4 2,186,960 - BWI76_RS10325 0.60 +1.0 2,187,013 - BWI76_RS10325 0.67 -1.0 2,187,013 - BWI76_RS10325 0.67 +0.3 2,187,042 - BWI76_RS10325 0.70 -0.1 2,187,067 + BWI76_RS10325 0.73 +0.1 2,187,067 + BWI76_RS10325 0.73 -0.6 2,187,075 + BWI76_RS10325 0.74 -3.6 2,187,090 - BWI76_RS10325 0.76 +0.2 2,187,131 - BWI76_RS10325 0.81 -0.1 2,187,187 - BWI76_RS10325 0.87 -0.2 2,187,187 - BWI76_RS10325 0.87 -2.5 2,187,242 - +0.8
Or see this region's nucleotide sequence