Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02330 and BWI76_RS02335 are separated by 25 nucleotides BWI76_RS02335 and BWI76_RS02340 are separated by 154 nucleotides BWI76_RS02340 and BWI76_RS02345 are separated by 15 nucleotides
BWI76_RS02330: BWI76_RS02330 - aspartate ammonia-lyase, at 479,414 to 480,850
_RS02330
BWI76_RS02335: BWI76_RS02335 - hypothetical protein, at 480,876 to 481,034
_RS02335
BWI76_RS02340: BWI76_RS02340 - membrane protein FxsA, at 481,189 to 481,665
_RS02340
BWI76_RS02345: BWI76_RS02345 - L-methionine/branched-chain amino acid transporter, at 481,681 to 482,940
_RS02345
Position (kb) 481
482 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 480.325 kb on + strand, within BWI76_RS02330 at 480.370 kb on + strand, within BWI76_RS02330 at 480.436 kb on - strand, within BWI76_RS02330 at 480.471 kb on + strand, within BWI76_RS02330 at 480.498 kb on - strand, within BWI76_RS02330 at 480.498 kb on - strand, within BWI76_RS02330 at 480.537 kb on + strand, within BWI76_RS02330 at 480.564 kb on - strand, within BWI76_RS02330 at 480.567 kb on + strand, within BWI76_RS02330 at 480.604 kb on - strand, within BWI76_RS02330 at 480.689 kb on - strand, within BWI76_RS02330 at 480.689 kb on - strand, within BWI76_RS02330 at 480.752 kb on + strand at 480.799 kb on + strand at 480.870 kb on - strand at 480.947 kb on + strand, within BWI76_RS02335 at 480.959 kb on + strand, within BWI76_RS02335 at 481.137 kb on + strand at 481.172 kb on + strand at 481.258 kb on + strand, within BWI76_RS02340 at 481.259 kb on + strand, within BWI76_RS02340 at 481.259 kb on + strand, within BWI76_RS02340 at 481.259 kb on + strand, within BWI76_RS02340 at 481.267 kb on - strand, within BWI76_RS02340 at 481.279 kb on - strand, within BWI76_RS02340 at 481.317 kb on - strand, within BWI76_RS02340 at 481.365 kb on + strand, within BWI76_RS02340 at 481.611 kb on + strand, within BWI76_RS02340 at 481.694 kb on + strand at 481.737 kb on + strand at 481.818 kb on - strand, within BWI76_RS02345 at 481.867 kb on + strand, within BWI76_RS02345 at 481.967 kb on - strand, within BWI76_RS02345 at 481.969 kb on + strand, within BWI76_RS02345 at 481.977 kb on - strand, within BWI76_RS02345 at 481.977 kb on - strand, within BWI76_RS02345 at 482.036 kb on + strand, within BWI76_RS02345 at 482.036 kb on + strand, within BWI76_RS02345 at 482.041 kb on + strand, within BWI76_RS02345 at 482.054 kb on + strand, within BWI76_RS02345 at 482.057 kb on - strand, within BWI76_RS02345 at 482.062 kb on - strand, within BWI76_RS02345 at 482.082 kb on - strand, within BWI76_RS02345 at 482.098 kb on + strand, within BWI76_RS02345 at 482.150 kb on + strand, within BWI76_RS02345 at 482.237 kb on - strand, within BWI76_RS02345 at 482.427 kb on + strand, within BWI76_RS02345 at 482.512 kb on + strand, within BWI76_RS02345 at 482.610 kb on - strand, within BWI76_RS02345 at 482.626 kb on + strand, within BWI76_RS02345 at 482.634 kb on - strand, within BWI76_RS02345 at 482.634 kb on - strand, within BWI76_RS02345
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 480,325 + BWI76_RS02330 0.63 +0.6 480,370 + BWI76_RS02330 0.67 -0.9 480,436 - BWI76_RS02330 0.71 -0.1 480,471 + BWI76_RS02330 0.74 -2.6 480,498 - BWI76_RS02330 0.75 +0.2 480,498 - BWI76_RS02330 0.75 +0.4 480,537 + BWI76_RS02330 0.78 -0.6 480,564 - BWI76_RS02330 0.80 +0.3 480,567 + BWI76_RS02330 0.80 +0.1 480,604 - BWI76_RS02330 0.83 -0.1 480,689 - BWI76_RS02330 0.89 +0.1 480,689 - BWI76_RS02330 0.89 +0.2 480,752 + +1.1 480,799 + -0.4 480,870 - -0.2 480,947 + BWI76_RS02335 0.45 -0.4 480,959 + BWI76_RS02335 0.52 -0.2 481,137 + -0.8 481,172 + +0.3 481,258 + BWI76_RS02340 0.14 -0.8 481,259 + BWI76_RS02340 0.15 -1.3 481,259 + BWI76_RS02340 0.15 +1.1 481,259 + BWI76_RS02340 0.15 -2.3 481,267 - BWI76_RS02340 0.16 -0.9 481,279 - BWI76_RS02340 0.19 -1.8 481,317 - BWI76_RS02340 0.27 -0.6 481,365 + BWI76_RS02340 0.37 -0.7 481,611 + BWI76_RS02340 0.88 -0.8 481,694 + +0.3 481,737 + +0.1 481,818 - BWI76_RS02345 0.11 +0.1 481,867 + BWI76_RS02345 0.15 +2.4 481,967 - BWI76_RS02345 0.23 +0.3 481,969 + BWI76_RS02345 0.23 +0.3 481,977 - BWI76_RS02345 0.23 -0.2 481,977 - BWI76_RS02345 0.23 +0.0 482,036 + BWI76_RS02345 0.28 -5.6 482,036 + BWI76_RS02345 0.28 +0.1 482,041 + BWI76_RS02345 0.29 -0.0 482,054 + BWI76_RS02345 0.30 +0.3 482,057 - BWI76_RS02345 0.30 -0.1 482,062 - BWI76_RS02345 0.30 -0.7 482,082 - BWI76_RS02345 0.32 -0.3 482,098 + BWI76_RS02345 0.33 +0.5 482,150 + BWI76_RS02345 0.37 +0.4 482,237 - BWI76_RS02345 0.44 +1.6 482,427 + BWI76_RS02345 0.59 -0.1 482,512 + BWI76_RS02345 0.66 -0.5 482,610 - BWI76_RS02345 0.74 +0.4 482,626 + BWI76_RS02345 0.75 +1.3 482,634 - BWI76_RS02345 0.76 +1.6 482,634 - BWI76_RS02345 0.76 +0.2
Or see this region's nucleotide sequence