Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01005 and BWI76_RS01010 are separated by 24 nucleotides BWI76_RS01010 and BWI76_RS01015 overlap by 1 nucleotides BWI76_RS01015 and BWI76_RS01020 are separated by 102 nucleotides
BWI76_RS01005: BWI76_RS01005 - CarD family transcriptional regulator, at 205,190 to 205,894
_RS01005
BWI76_RS01010: BWI76_RS01010 - uridine phosphorylase, at 205,919 to 206,668
_RS01010
BWI76_RS01015: BWI76_RS01015 - nicotinamide riboside transporter PnuC, at 206,668 to 207,390
_RS01015
BWI76_RS01020: BWI76_RS01020 - DNA helicase Rep, at 207,493 to 209,511
_RS01020
Position (kb) 206
207
208 Strain fitness (log2 ratio) -1
0
1
2 at 205.812 kb on - strand, within BWI76_RS01005 at 205.812 kb on - strand, within BWI76_RS01005 at 205.938 kb on - strand at 206.028 kb on + strand, within BWI76_RS01010 at 206.077 kb on + strand, within BWI76_RS01010 at 206.081 kb on - strand, within BWI76_RS01010 at 206.201 kb on + strand, within BWI76_RS01010 at 206.456 kb on + strand, within BWI76_RS01010 at 206.456 kb on + strand, within BWI76_RS01010 at 206.464 kb on - strand, within BWI76_RS01010 at 206.464 kb on - strand, within BWI76_RS01010 at 206.464 kb on - strand, within BWI76_RS01010 at 206.993 kb on + strand, within BWI76_RS01015 at 207.049 kb on + strand, within BWI76_RS01015 at 207.203 kb on - strand, within BWI76_RS01015 at 207.373 kb on - strand at 207.399 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 205,812 - BWI76_RS01005 0.88 +0.8 205,812 - BWI76_RS01005 0.88 +0.6 205,938 - +2.7 206,028 + BWI76_RS01010 0.15 -0.0 206,077 + BWI76_RS01010 0.21 +0.4 206,081 - BWI76_RS01010 0.22 +0.9 206,201 + BWI76_RS01010 0.38 +2.6 206,456 + BWI76_RS01010 0.72 -0.9 206,456 + BWI76_RS01010 0.72 -0.5 206,464 - BWI76_RS01010 0.73 +0.6 206,464 - BWI76_RS01010 0.73 +0.4 206,464 - BWI76_RS01010 0.73 -0.5 206,993 + BWI76_RS01015 0.45 +0.1 207,049 + BWI76_RS01015 0.53 +1.1 207,203 - BWI76_RS01015 0.74 +0.9 207,373 - -0.9 207,399 + +0.0
Or see this region's nucleotide sequence