Experiment: MoYLS4 with Chlorite 100uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cobB-2 and cobH are separated by 8 nucleotides cobH and deaD are separated by 108 nucleotides
DVU3086: cobB-2 - cobyrinic acid a,c-diamide synthase (TIGR), at 3,227,312 to 3,229,135
cobB-2
DVU3087: cobH - precorrin-8X methylmutase (TIGR), at 3,229,144 to 3,229,785
cobH
DVU3088: deaD - ATP-dependent RNA helicase, DEAD/DEAH box family (TIGR), at 3,229,894 to 3,231,627
deaD
Position (kb)
3229
3230 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3228.215 kb on - strand, within cobB-2 at 3228.238 kb on + strand, within cobB-2 at 3228.246 kb on - strand, within cobB-2 at 3228.246 kb on - strand, within cobB-2 at 3228.283 kb on - strand, within cobB-2 at 3228.285 kb on + strand, within cobB-2 at 3228.300 kb on - strand, within cobB-2 at 3228.300 kb on - strand, within cobB-2 at 3228.486 kb on - strand, within cobB-2 at 3228.523 kb on - strand, within cobB-2 at 3228.563 kb on + strand, within cobB-2 at 3228.571 kb on - strand, within cobB-2 at 3228.580 kb on + strand, within cobB-2 at 3228.618 kb on + strand, within cobB-2 at 3228.634 kb on + strand, within cobB-2 at 3228.650 kb on - strand, within cobB-2 at 3228.706 kb on + strand, within cobB-2 at 3228.804 kb on + strand, within cobB-2 at 3228.876 kb on - strand, within cobB-2 at 3228.910 kb on - strand, within cobB-2 at 3228.910 kb on - strand, within cobB-2 at 3228.910 kb on - strand, within cobB-2 at 3228.910 kb on - strand, within cobB-2 at 3228.918 kb on + strand, within cobB-2 at 3228.921 kb on + strand, within cobB-2 at 3228.983 kb on + strand at 3228.991 kb on - strand at 3229.020 kb on + strand at 3229.020 kb on + strand at 3229.020 kb on + strand at 3229.104 kb on - strand at 3229.118 kb on + strand at 3229.128 kb on + strand at 3229.153 kb on + strand at 3229.276 kb on - strand, within cobH at 3229.281 kb on - strand, within cobH at 3229.281 kb on - strand, within cobH at 3229.281 kb on - strand, within cobH at 3229.312 kb on + strand, within cobH at 3229.312 kb on + strand, within cobH at 3229.408 kb on - strand, within cobH at 3229.423 kb on + strand, within cobH at 3229.489 kb on + strand, within cobH at 3229.579 kb on - strand, within cobH at 3229.604 kb on - strand, within cobH at 3229.610 kb on + strand, within cobH at 3229.613 kb on - strand, within cobH at 3229.618 kb on - strand, within cobH at 3229.647 kb on - strand, within cobH at 3229.691 kb on + strand, within cobH at 3229.691 kb on + strand, within cobH at 3229.694 kb on - strand, within cobH at 3229.852 kb on + strand at 3229.859 kb on - strand at 3229.898 kb on + strand at 3229.966 kb on + strand at 3230.011 kb on - strand at 3230.044 kb on + strand at 3230.059 kb on + strand at 3230.092 kb on + strand, within deaD at 3230.093 kb on - strand, within deaD at 3230.097 kb on + strand, within deaD at 3230.100 kb on - strand, within deaD at 3230.136 kb on - strand, within deaD at 3230.175 kb on - strand, within deaD at 3230.178 kb on + strand, within deaD at 3230.197 kb on + strand, within deaD at 3230.308 kb on + strand, within deaD at 3230.337 kb on + strand, within deaD at 3230.349 kb on + strand, within deaD at 3230.406 kb on + strand, within deaD at 3230.421 kb on + strand, within deaD at 3230.448 kb on + strand, within deaD at 3230.462 kb on + strand, within deaD at 3230.566 kb on + strand, within deaD at 3230.567 kb on + strand, within deaD at 3230.682 kb on + strand, within deaD at 3230.689 kb on + strand, within deaD at 3230.747 kb on - strand, within deaD
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Chlorite 100uM remove 3,228,215 - cobB-2 DVU3086 0.50 +1.3 3,228,238 + cobB-2 DVU3086 0.51 -0.0 3,228,246 - cobB-2 DVU3086 0.51 +0.0 3,228,246 - cobB-2 DVU3086 0.51 +1.6 3,228,283 - cobB-2 DVU3086 0.53 -1.2 3,228,285 + cobB-2 DVU3086 0.53 -1.0 3,228,300 - cobB-2 DVU3086 0.54 -1.8 3,228,300 - cobB-2 DVU3086 0.54 -0.8 3,228,486 - cobB-2 DVU3086 0.64 -0.3 3,228,523 - cobB-2 DVU3086 0.66 -0.3 3,228,563 + cobB-2 DVU3086 0.69 -0.5 3,228,571 - cobB-2 DVU3086 0.69 -2.0 3,228,580 + cobB-2 DVU3086 0.70 -2.8 3,228,618 + cobB-2 DVU3086 0.72 -1.4 3,228,634 + cobB-2 DVU3086 0.72 -0.9 3,228,650 - cobB-2 DVU3086 0.73 +0.2 3,228,706 + cobB-2 DVU3086 0.76 -0.0 3,228,804 + cobB-2 DVU3086 0.82 -1.3 3,228,876 - cobB-2 DVU3086 0.86 -1.9 3,228,910 - cobB-2 DVU3086 0.88 +0.9 3,228,910 - cobB-2 DVU3086 0.88 -1.1 3,228,910 - cobB-2 DVU3086 0.88 -0.0 3,228,910 - cobB-2 DVU3086 0.88 +2.3 3,228,918 + cobB-2 DVU3086 0.88 -0.8 3,228,921 + cobB-2 DVU3086 0.88 -1.3 3,228,983 + -0.9 3,228,991 - +0.5 3,229,020 + +0.4 3,229,020 + -1.0 3,229,020 + -0.9 3,229,104 - -0.3 3,229,118 + +1.0 3,229,128 + -0.2 3,229,153 + -0.2 3,229,276 - cobH DVU3087 0.21 -1.7 3,229,281 - cobH DVU3087 0.21 -1.6 3,229,281 - cobH DVU3087 0.21 +0.5 3,229,281 - cobH DVU3087 0.21 -0.1 3,229,312 + cobH DVU3087 0.26 -1.1 3,229,312 + cobH DVU3087 0.26 +0.4 3,229,408 - cobH DVU3087 0.41 -1.1 3,229,423 + cobH DVU3087 0.43 -1.1 3,229,489 + cobH DVU3087 0.54 +1.3 3,229,579 - cobH DVU3087 0.68 -0.9 3,229,604 - cobH DVU3087 0.72 -1.0 3,229,610 + cobH DVU3087 0.73 -0.1 3,229,613 - cobH DVU3087 0.73 -0.9 3,229,618 - cobH DVU3087 0.74 -1.0 3,229,647 - cobH DVU3087 0.78 -1.0 3,229,691 + cobH DVU3087 0.85 -1.4 3,229,691 + cobH DVU3087 0.85 -0.3 3,229,694 - cobH DVU3087 0.86 -1.1 3,229,852 + +2.3 3,229,859 - -0.2 3,229,898 + +0.1 3,229,966 + -0.3 3,230,011 - -1.3 3,230,044 + -0.3 3,230,059 + +0.3 3,230,092 + deaD DVU3088 0.11 -0.1 3,230,093 - deaD DVU3088 0.11 +0.1 3,230,097 + deaD DVU3088 0.12 +0.0 3,230,100 - deaD DVU3088 0.12 +0.6 3,230,136 - deaD DVU3088 0.14 +0.9 3,230,175 - deaD DVU3088 0.16 -1.2 3,230,178 + deaD DVU3088 0.16 -0.5 3,230,197 + deaD DVU3088 0.17 -1.4 3,230,308 + deaD DVU3088 0.24 -1.0 3,230,337 + deaD DVU3088 0.26 -1.3 3,230,349 + deaD DVU3088 0.26 +1.3 3,230,406 + deaD DVU3088 0.30 +0.1 3,230,421 + deaD DVU3088 0.30 +0.3 3,230,448 + deaD DVU3088 0.32 -0.2 3,230,462 + deaD DVU3088 0.33 -0.1 3,230,566 + deaD DVU3088 0.39 -1.9 3,230,567 + deaD DVU3088 0.39 -0.0 3,230,682 + deaD DVU3088 0.45 -0.6 3,230,689 + deaD DVU3088 0.46 -1.0 3,230,747 - deaD DVU3088 0.49 -0.9
Or see this region's nucleotide sequence