Experiment: MoYLS4 with Chlorite 100uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b2488 and DVU2290 overlap by 4 nucleotides DVU2290 and DVU2291 overlap by 4 nucleotides DVU2291 and hypA are separated by 8 nucleotides hypA and cooF overlap by 4 nucleotides
DVU2289: b2488 - hydrogenase, CooX subunit, putative (TIGR), at 2,382,468 to 2,383,103
b2488
DVU2290: DVU2290 - hydrogenase, CooU subunit, putative (TIGR), at 2,383,100 to 2,383,645
DVU2290
DVU2291: DVU2291 - carbon monoxide-induced hydrogenase CooH, putative (TIGR), at 2,383,642 to 2,384,742
DVU2291
DVU2292: hypA - hydrogenase nickel insertion protein HypA (TIGR), at 2,384,751 to 2,385,113
hypA
DVU2293: cooF - iron-sulfur protein CooF (TIGR), at 2,385,110 to 2,385,631
cooF
Position (kb)
2383
2384
2385 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2382.750 kb on - strand, within b2488 at 2382.926 kb on + strand, within b2488 at 2383.041 kb on + strand at 2383.041 kb on + strand at 2383.041 kb on + strand at 2383.116 kb on + strand at 2383.243 kb on + strand, within DVU2290 at 2383.365 kb on + strand, within DVU2290 at 2383.639 kb on + strand at 2383.775 kb on + strand, within DVU2291 at 2383.819 kb on - strand, within DVU2291 at 2384.062 kb on + strand, within DVU2291 at 2384.276 kb on - strand, within DVU2291 at 2384.352 kb on + strand, within DVU2291 at 2384.556 kb on + strand, within DVU2291 at 2384.716 kb on - strand at 2384.850 kb on + strand, within hypA at 2384.863 kb on - strand, within hypA at 2384.876 kb on + strand, within hypA at 2384.949 kb on + strand, within hypA at 2385.084 kb on - strand at 2385.304 kb on + strand, within cooF at 2385.519 kb on + strand, within cooF at 2385.568 kb on + strand, within cooF at 2385.592 kb on - strand at 2385.639 kb on - strand at 2385.702 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Chlorite 100uM remove 2,382,750 - b2488 DVU2289 0.44 -0.4 2,382,926 + b2488 DVU2289 0.72 -1.0 2,383,041 + -1.2 2,383,041 + +0.3 2,383,041 + -0.6 2,383,116 + -2.0 2,383,243 + DVU2290 0.26 +0.6 2,383,365 + DVU2290 0.49 +0.4 2,383,639 + -0.2 2,383,775 + DVU2291 0.12 -1.0 2,383,819 - DVU2291 0.16 -0.9 2,384,062 + DVU2291 0.38 -0.8 2,384,276 - DVU2291 0.58 +0.6 2,384,352 + DVU2291 0.64 +0.4 2,384,556 + DVU2291 0.83 -0.9 2,384,716 - -0.2 2,384,850 + hypA DVU2292 0.27 -1.7 2,384,863 - hypA DVU2292 0.31 +0.3 2,384,876 + hypA DVU2292 0.34 -2.0 2,384,949 + hypA DVU2292 0.55 -1.0 2,385,084 - +0.1 2,385,304 + cooF DVU2293 0.37 -3.0 2,385,519 + cooF DVU2293 0.78 -2.1 2,385,568 + cooF DVU2293 0.88 -1.9 2,385,592 - -0.8 2,385,639 - -0.6 2,385,702 + -0.3
Or see this region's nucleotide sequence